anti-Ncd N-terminal tail
Catalog Fields
Clone ID/Product Name: anti-Ncd N-terminal tail
Available to For-Profits: No
Alternate Antibody Name:
Gene Symbol: KIF14
Ab Isotype: Rabbit IgG
Gene Name:
Antibody Registry ID: AB_3722704
Clonality: Polyclonal
Immunogen:
Clone:
Immunogen Sequence:
Myeloma Strain:
Epitope Mapped: No
Antigen Name: Kinesin-14 Ncd 1-204 aa
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 1/13/2026
Antigen Molecular Weight:
Depositor: Endow, S.
Antigen Sequence:
Depositor Institution: Duke University
Antigen Species:
Depositor Notes: After protein A purification, Ab at ~0.1 mg/ml was diluted 1:200 for Western blots and was used at higher concentration (1:3 or 1:5 dilution) for oocyte or embryo staining. Note that the above dilutions are for purified Ab and were approximated from notes; users would have to determine dilutions for sera or if they purified Ab from the sera. However, both anti-Ncd Ab cross-react with Ncd on Western blots and in fixed spindles.
Host Species: rabbit
Hybridoma Cells Available (Non-Profit): No
Confirmed Species Reactivity:
Additional Information:
Recommended Applications: Immunofluorescence, Immunohistochemistry, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
anti-Ncd N-terminal tail was deposited to the DSHB by Endow, S. (DSHB Hybridoma Product anti-Ncd N-terminal tail)
Storage and Handling Recommendations
Although many cell products are maintained at 4?C for years without loss of activity, shelf-life at 4?C is highly variable. For immediate use, short term storage at 4?C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 µl for freezing at -20?C or -80?C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 µg/ml. For western blots, the recommended concentration range is 0.2-0.5 µg/ml.
1 Reference
Initial Publication
The Drosophila ncd microtubule motor protein is spindle-associated in meiotic and mitotic cells.
Endow SA
Journal of cell science 103 ( Pt 4). (1992 Dec): 1013-20.