antiTau_pT217 [E55b4]
Catalog Fields
Clone ID/Product Name: antiTau_pT217 [E55b4]
Available to For-Profits: No
Alternate Antibody Name: MEAH7m_E55b4
Gene Symbol: MAPT
Ab Isotype: MIgG2b, kappa light chain
Gene Name:
Antibody Registry ID: AB_3714763
Uniprot ID: P10636
Entrez Gene ID: 4137
Clonality: Monoclonal
Immunogen: Tau pT217 Tryptic Peptide
Clone:
Immunogen Sequence: TPSLPpTPPTREPK
Myeloma Strain:
Epitope Mapped: No
Antigen Name: TPSLPpTPPTREPK
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 9/9/2025
Antigen Molecular Weight: 1500.58
Depositor: Hoofnagle, A.
Antigen Sequence:
Depositor Institution: University of Washington
Antigen Species: Human
Depositor Notes: Specific to phosphorylated site
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Human
Additional Information:
Predicted Species Reactivity:
Human Protein Atlas: https://www.poteinatlas.org/ENSG00000186868-MAPT
Recommended Applications: ELISA, Immuno-MRM, Immuno-MS, Immunohistochemistry, Immunoprecipitation
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
antiTau_pT217 [E55b4] was deposited to the DSHB by Hoofnagle, A. (DSHB Hybridoma Product antiTau_pT217 [E55b4])
Storage and Handling Recommendations
Although many cell products are maintained at 4?C for years without loss of activity, shelf-life at 4?C is highly variable. For immediate use, short term storage at 4?C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 µl for freezing at -20?C or -80?C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 µg/ml. For western blots, the recommended concentration range is 0.2-0.5 µg/ml.
1 Reference
Initial Publication
Performance of novel tau antibodies across multiple modalities for Alzheimer's disease assessment.
Latimer CS
Alzheimer's & dementia : the journal of the Alzheimer's Association 21.7 (2025 Jul): e70481.