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SKU: b29

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DSHB Data Sheet

Catalog Fields

Product Name/ID: b29
Available to For-Profits: Yes
Alternate Antibody Name: beta29; mAb 29
Gene Name: ATP1B1
Ab Isotype: MIgG1, kappa light chain
Gene Symbol:
Antibody Registry ID: AB_528096 
Uniprot ID: P08251 
Entrez Gene ID: 396529 
Clonality: Monoclonal
Immunogen: purified adult chicken kidney (Na+, K+)-ATPase beta-subunit.
Immunogen Sequence: Total protein
Myeloma Strain: SP2/0 Ag8
Epitope Mapped: No
Antigen Name: ATPase, (Na(+) K(+)) beta-1 subunit (avian)
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 12/1/1998
Antigen Molecular Weight: Predicted: 35 kDa; Apparent: broad band at 45-60 kDa
Depositor: Fambrough, D.M.
Antigen Sequence:
Depositor Institution: Johns Hopkins School of Medicine
Antigen Species: Chicken
Depositor Notes: The epitope appears to be linear. Does not bind native beta subunits on cells, but does bind to some C-terminally truncated forms. This antibody preferentially recognizes partially denatured beta subunits.. This antibody is excellent for Immunoblotting but does not work for Immunoprecipitation or Immunohistochemistry. Cros-reactivity with mammalian is unlikely. Weaker than mAb 24 for immunoprecipitation of native beta subunit, but better than mAb 24 for truncated subunit [PMID 7901216].
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Avian
Additional Information:
Predicted Species Reactivity:  
Human Protein Atlas:  
Additional Characterization:  
Recommended Applications: Immunofluorescence, Immunoprecipitation, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
b29 was deposited to the DSHB by Fambrough, D.M. (DSHB Hybridoma Product b29)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

4 References

  • Initial Publication
  • IF References
  • WB References
  • IP References
  • All References
  • Initial Publication
    IF References
    WB References

    Cytoplasmic and transmembrane domain deletions of Na,K-ATPase beta-subunit. Effects on subunit assembly and intracellular transport.
    Fambrough DM
    The Journal of biological chemistry 266.30 (1991 Oct 25): 20491-7.

    Expression of the avian Na,K-ATPase subunits in Dictyostelium discoideum.
    Fambrough DM
    The Journal of membrane biology 167.1 (1999 Jan 1): 19-24.

    IP References
    All References

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