Catalog Fields
Antigen: ATPase, (Na(+) K(+)) beta-1 subunit (avian)
Hybridoma Cells Available: Yes
Antigen Species: Chicken
Depositor: Fambrough, D.M.
Isotype: MIgG1, kappa light chain
Antigen Sequence:
Host Species: mouse
Depositors Institution: Johns Hopkins School of Medicine
Positive Tested Species Reactivity: Avian
Depositors Notes: The epitope appears to be linear. Does not bind native beta subunits on cells, but does bind to some C-terminally truncated forms. This antibody preferentially recognizes partially denatured beta subunits.. This antibody is excellent for Immunoblotting but does not work for Immunoprecipitation or Immunohistochemistry. Cros-reactivity with mammalian is unlikely. Weaker than mAb 24 for immunoprecipitation of native beta subunit, but better than mAb 24 for truncated subunit [PMID 7901216].
Predicted Species Reactivity:
Gene: ATP1B1
Immunogen: purified adult chicken kidney (Na+, K+)-ATPase beta-subunit.
Alternate Gene Names:
Alternate Antibody Name: beta29; mAb 29
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: SP2/0 Ag8
Epitope Location or Sequence:
Uniprot ID: P08251
Immunogen Sequence: Total protein
Entrez Gene ID: 396529
Antibody Registry ID: AB_528096
Additional Information:
Recommended Applications: Immunofluorescence, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
b29 was deposited to the DSHB by Fambrough, D.M. (DSHB Hybridoma Product b29)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
All cell products contain the antimicrobial ProClin. Click here for additional information.
4 References
Initial Publication
Cytoplasmic and transmembrane domain deletions of Na,K-ATPase beta-subunit. Effects on subunit assembly and intracellular transport.
Fambrough DM
The Journal of biological chemistry 266.30 (1991 Oct 25): 20491-7.
Assembly of the extracellular domain of the Na,K-ATPase beta subunit with the alpha subunit. Analysis of beta subunit chimeras and carboxyl-terminal deletions.
Fambrough DM
The Journal of biological chemistry 268.32 (1993 Nov 15): 24367-73.