b29
Catalog Fields
Antigen: ATPase, (Na(+) K(+)) beta-1 subunit (avian)
Hybridoma Cells Available: Yes
Antigen Species: Chicken
Depositor: Fambrough, D.M.
Isotype: MIgG1, kappa light chain
Antigen Sequence:
Host Species: mouse
Depositors Institution: Johns Hopkins School of Medicine
Positive Tested Species Reactivity: Avian
Depositors Notes: The epitope appears to be linear. Does not bind native beta subunits on cells, but does bind to some C-terminally truncated forms. This antibody preferentially recognizes partially denatured beta subunits.. This antibody is excellent for Immunoblotting but does not work for Immunoprecipitation or Immunohistochemistry. Cros-reactivity with mammalian is unlikely. Weaker than mAb 24 for immunoprecipitation of native beta subunit, but better than mAb 24 for truncated subunit [PMID 7901216].
Antigen Molecular Weight: Predicted: 35 kDa; Apparent: broad band at 45-60 kDa
Human Protein Atlas:
Predicted Species Reactivity:
Gene: ATP1B1
Immunogen: purified adult chicken kidney (Na+, K+)-ATPase beta-subunit.
Alternate Gene Names:
Alternate Antibody Name: beta29; mAb 29
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped:
Myeloma Strain: SP2/0 Ag8
Epitope Location or Sequence:
Uniprot ID: P08251
Immunogen Sequence: Total protein
Entrez Gene ID: 396529
Additional Characterization:
Antibody Registry ID: AB_528096
Additional Information:
Recommended Applications: Immunofluorescence, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
b29 was deposited to the DSHB by Fambrough, D.M. (DSHB Hybridoma Product b29)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.
4 References
Initial Publication
Cytoplasmic and transmembrane domain deletions of Na,K-ATPase beta-subunit. Effects on subunit assembly and intracellular transport.
Fambrough DM
The Journal of biological chemistry 266.30 (1991 Oct 25): 20491-7.
Assembly of the extracellular domain of the Na,K-ATPase beta subunit with the alpha subunit. Analysis of beta subunit chimeras and carboxyl-terminal deletions.
Fambrough DM
The Journal of biological chemistry 268.32 (1993 Nov 15): 24367-73.