b29
Catalog Fields
Clone ID/Product Name: b29
Available to For-Profits: Yes
Alternate Antibody Name: beta29; mAb 29
Gene Symbol: ATP1B1
Ab Isotype: MIgG1, kappa light chain
Gene Name:
Entrez Gene ID: 396529
Clonality: Monoclonal
Immunogen: purified adult chicken kidney (Na+, K+)-ATPase beta-subunit.
Clone:
Immunogen Sequence: Total protein
Myeloma Strain: SP2/0 Ag8
Epitope Mapped: No
Antigen Name: ATPase, (Na(+) K(+)) beta-1 subunit (avian)
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 12/1/1998
Antigen Molecular Weight: Predicted: 35 kDa; Apparent: broad band at 45-60 kDa
Depositor: Fambrough, D.M.
Antigen Sequence:
Depositor Institution: Johns Hopkins School of Medicine
Antigen Species: Chicken
Depositor Notes: The epitope appears to be linear. Does not bind native beta subunits on cells, but does bind to some C-terminally truncated forms. This antibody preferentially recognizes partially denatured beta subunits.. This antibody is excellent for Immunoblotting but does not work for Immunoprecipitation or Immunohistochemistry. Cros-reactivity with mammalian is unlikely. Weaker than mAb 24 for immunoprecipitation of native beta subunit, but better than mAb 24 for truncated subunit [PMID 7901216].
Host Species: Mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Avian
Additional Information:
Recommended Applications: Immunofluorescence, Immunoprecipitation, Western Blot