d1C4

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$40.00
SKU: d1C4
View product citations for antibody d1C4 on CiteAb

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Available: 40

DSHB Data Sheet

Catalog Fields

Antigen: chondrotin-6-sulfate (CS-C)
Hybridoma Cells Available: Yes
Antigen Species: Chicken
Depositor: Capehart, A.A.
Isotype: MIgM
Antigen Sequence:
Host Species: mouse
Depositors Institution: East Carolina University
Positive Tested Species Reactivity: Chicken
Depositors Notes: Dent's fixative (80% methanol-20% DMSO) followed by paraffin sections is recommended.
Antigen Molecular Weight:
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene:
Immunogen: Mesenchymal micromass culture
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: SP2/0
Epitope Location or Sequence: CS-C
Uniprot ID:  
Immunogen Sequence:
Entrez Gene ID:  
Additional Characterization:  
Antibody Registry ID: AB_2801368 
Additional Information:
Recommended Applications: ELISA, Immunofluorescence
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
d1C4 was deposited to the DSHB by Capehart, A.A. (DSHB Hybridoma Product d1C4)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

1 Reference

  • Initial Publication
  • IF References
  • ELISA References
  • Epitope Map References
  • All References
  • Initial Publication

    Production of a monoclonal antibody by in vitro immunization that recognizes a native chondroitin sulfate epitope in the embryonic chick limb and heart.
    Krug EL
    The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 45.11 (1997 Nov): 1567-81.

    IF References

    Production of a monoclonal antibody by in vitro immunization that recognizes a native chondroitin sulfate epitope in the embryonic chick limb and heart.
    Krug EL
    The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 45.11 (1997 Nov): 1567-81.

    ELISA References

    Production of a monoclonal antibody by in vitro immunization that recognizes a native chondroitin sulfate epitope in the embryonic chick limb and heart.
    Krug EL
    The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 45.11 (1997 Nov): 1567-81.

    Epitope Map References

    Production of a monoclonal antibody by in vitro immunization that recognizes a native chondroitin sulfate epitope in the embryonic chick limb and heart.
    Krug EL
    The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 45.11 (1997 Nov): 1567-81.

    All References

    Production of a monoclonal antibody by in vitro immunization that recognizes a native chondroitin sulfate epitope in the embryonic chick limb and heart.
    Krug EL
    The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 45.11 (1997 Nov): 1567-81.

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