fasciclin II 2F5

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$40.00
SKU: fasciclin II 2F5
View product citations for antibody fasciclin II 2F5 on CiteAb

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Available: 6

DSHB Data Sheet

Catalog Fields

Antigen: fasciclin II (Manduca sexta)
Hybridoma Cells Available: Yes
Antigen Species: Manduca sexta
Depositor: Nardi, J.
Isotype: MIgG1, kappa light chain
Antigen Sequence:
Host Species: mouse
Depositors Institution: Department of Entomology, University of Illinois
Positive Tested Species Reactivity: Manduca sexta
Depositors Notes:
Antigen Molecular Weight: Apparent: 91 kDa and 94 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: Fas2
Immunogen: Manduca sexta adult transverse nerve
Alternate Gene Names: FasII
Alternate Antibody Name: TN-1
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: Sp2/0
Epitope Location or Sequence:
Uniprot ID:  
Immunogen Sequence: Total protein
Entrez Gene ID:  
Additional Characterization:  
Antibody Registry ID: AB_10805878 
Additional Information: Works on western blots under nonreducing conditions.
Recommended Applications: Function Blocking, Immunofluorescence, Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
fasciclin II 2F5 was deposited to the DSHB by Nardi, J. (DSHB Hybridoma Product fasciclin II 2F5)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

5 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • FB References
  • All References
  • Initial Publication
    IF References
    WB References
    IHC References
    FB References
    All References

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