mAB 197

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$40.00
SKU: mAB 197
View product citations for antibody mAB 197 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: High affinity IgG Fc receptor I/ CD64
Hybridoma Cells Available: Yes
Antigen Species: Human
Depositor: Guyre, Paul M.
Isotype: MIgG2a
Antigen Sequence:
Host Species: mouse
Depositors Institution: Geisel School of Medicine at Dartmouth
Positive Tested Species Reactivity: Human
Depositors Notes: mAb 197 triggers a oxidative burst response
Antigen Molecular Weight: 42.6 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: FCGR1A
Immunogen: Mononuclear leukocytes
Alternate Gene Names: CD64, CD64A, FCG1, FCGR1, IGFR1
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: NS-1
Epitope Location or Sequence: mAB 197 binds to FcyRl by both its FC and F(ab) domains; trivalent
Uniprot ID: P12314 
Immunogen Sequence: Full length protein
Entrez Gene ID: 2209 
Additional Characterization:  
Antibody Registry ID: AB_2801367 
Additional Information: Patent 4,954,617. Monoclonal antibodies to FC receptors for immunoglobulin G on human mononuclear phagocytes
Recommended Applications: ELISA, FACS, Function Blocking, Immunofluorescence, Immunoprecipitation
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
mAB 197 was deposited to the DSHB by Guyre, Paul M. (DSHB Hybridoma Product mAB 197)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

12 References

  • Initial Publication
  • IF References
  • IP References
  • ELISA References
  • FB References
  • FACS References
  • Epitope Map References
  • All References
  • Initial Publication
    IF References
    IP References

    The cytoplasmic domain of human FcgammaRIa alters the functional properties of the FcgammaRI.gamma-chain receptor complex.
    Kimberly RP
    The Journal of biological chemistry 274.42 (1999 Oct 15): 30328-33.

    Physical and functional association of the high affinity immunoglobulin G receptor (Fc gamma RI) with the kinases Hck and Lyn.
    Geha RS
    The Journal of experimental medicine 180.3 (1994 Sep 1): 1165-70.

    Association of the high-affinity receptor for IgG (Fc gamma RI) with the gamma subunit of the IgE receptor.
    Anderson CL
    Proceedings of the National Academy of Sciences of the United States of America 90.13 (1993 Jul 1): 6023-7.

    ELISA References
    FB References
    FACS References
    Epitope Map References

    Monoclonal antibodies that bind to distinct epitopes on Fc gamma RI are able to trigger receptor function.
    Fanger MW
    Journal of immunology (Baltimore, Md. : 1950) 143.5 (1989 Sep 1): 1650-5.

    All References

    Monoclonal antibody 197 (anti-Fc gamma RI) infusion in a patient with immune thrombocytopenia purpura (ITP) results in down-modulation of Fc gamma RI on circulating monocytes.
    Ely P
    British journal of haematology 92.3 (1996 Mar): 718-24.

    Neutrophil activation through high-affinity Fc gamma receptor using a monomeric antibody with unique properties.
    Davis BH
    Blood 77.3 (1991 Feb 1): 607-15.

    The cytoplasmic domain of human FcgammaRIa alters the functional properties of the FcgammaRI.gamma-chain receptor complex.
    Kimberly RP
    The Journal of biological chemistry 274.42 (1999 Oct 15): 30328-33.

    Physical and functional association of the high affinity immunoglobulin G receptor (Fc gamma RI) with the kinases Hck and Lyn.
    Geha RS
    The Journal of experimental medicine 180.3 (1994 Sep 1): 1165-70.

    Association of the high-affinity receptor for IgG (Fc gamma RI) with the gamma subunit of the IgE receptor.
    Anderson CL
    Proceedings of the National Academy of Sciences of the United States of America 90.13 (1993 Jul 1): 6023-7.

    Efficient carcinoma cell killing by activated polymorphonuclear neutrophils targeted with an Ep-CAMxCD64 (HEA125x197) bispecific antibody.
    Moldenhauer G
    Cancer immunology, immunotherapy : CII 51.11-12 (2002 Dec): 621-9.

    Monoclonal antibodies that bind to distinct epitopes on Fc gamma RI are able to trigger receptor function.
    Fanger MW
    Journal of immunology (Baltimore, Md. : 1950) 143.5 (1989 Sep 1): 1650-5.

    Cross-linking of the high affinity Fc receptor for human immunoglobulin G1 triggers transient activation of NADPH oxidase activity. Continuous oxidase activation requires continuous de novo receptor cross-linking.
    Fanger MW
    The Journal of biological chemistry 264.24 (1989 Aug 25): 14112-20.

    Enhanced antigen presentation using human Fc gamma receptor (monocyte/macrophage)-specific immunogens.
    Guyre PM
    Journal of immunology (Baltimore, Md. : 1950) 149.11 (1992 Dec 1): 3477-81.

    Involvement of the high-affinity receptor for IgG (Fc gamma RI; CD64) in enhanced tumor cell cytotoxicity of neutrophils during granulocyte colony-stimulating factor therapy.
    van de Winkel JG
    Blood 82.3 (1993 Aug 1): 931-9.

    Receptor modulation by Fc gamma RI-specific fusion proteins is dependent on receptor number and modified by IgG.
    Fanger MW
    Journal of immunology (Baltimore, Md. : 1950) 167.11 (2001 Dec 1): 6303-11.

    Single step screening of monoclonal antibodies against interferon-gamma-induced surface molecules on human monocytes.
    Guyre PM
    Journal of immunological methods 118.2 (1989 Mar 31): 287-96.

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