mAB 1B7

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SKU: mAB 1B7

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DSHB Data Sheet

Catalog Fields

Product Name/ID: mAB 1B7
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Name:
Ab Isotype: MIgG1
Gene Symbol:
Antibody Registry ID: AB_2722115 
Uniprot ID: P18614 
RRID:  
Entrez Gene ID: 25118 
Clonality: Monoclonal
Immunogen: PC12 cells
Clone:
Immunogen Sequence:
Myeloma Strain: NS-1
Epitope Mapped: No
Antigen Name: Control for mAb 3A3
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 9/29/2015
Antigen Molecular Weight: This antibody reacts with multiple proteins bands.
Depositor: Carbonetto, Salvatore / Turner, D.C.
Antigen Sequence:
Depositor Institution: McGill University
Antigen Species: Rat
Depositor Notes: mAB 1B1 is a control antibody for MAB 3A3. This antibody recognizes multiple cell surface antigens but does not block function in PC12 cells. mAB 1B1 promotes passive detachment from WGA or polylysine.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Rat
Additional Information: RRID:AB_2722115
Predicted Species Reactivity:  
Human Protein Atlas:  
Additional Characterization:  
Recommended Applications: Immunofluorescence, Immunohistochemistry, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
mAB 1B7 was deposited to the DSHB by Carbonetto, Salvatore / Turner, D.C. (DSHB Hybridoma Product mAB 1B7)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

1 Reference

  • Initial Publication
  • IF References
  • WB References
  • All References
  • Initial Publication

    Identification of a cell-surface protein involved in PC12 cell-substratum adhesion and neurite outgrowth on laminin and collagen.
    Carbonetto S
    The Journal of neuroscience : the official journal of the Society for Neuroscience 9.9 (1989 Sep): 3287-96.

    IF References

    Identification of a cell-surface protein involved in PC12 cell-substratum adhesion and neurite outgrowth on laminin and collagen.
    Carbonetto S
    The Journal of neuroscience : the official journal of the Society for Neuroscience 9.9 (1989 Sep): 3287-96.

    WB References

    Identification of a cell-surface protein involved in PC12 cell-substratum adhesion and neurite outgrowth on laminin and collagen.
    Carbonetto S
    The Journal of neuroscience : the official journal of the Society for Neuroscience 9.9 (1989 Sep): 3287-96.

    All References

    Identification of a cell-surface protein involved in PC12 cell-substratum adhesion and neurite outgrowth on laminin and collagen.
    Carbonetto S
    The Journal of neuroscience : the official journal of the Society for Neuroscience 9.9 (1989 Sep): 3287-96.

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