mAB 3A3
Catalog Fields
Clone ID/Product Name: mAB 3A3
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Symbol: Itga1
Ab Isotype: MIgG1
Gene Name:
Antibody Registry ID: AB_2722115
Uniprot ID: P18614
Entrez Gene ID: 25118
Clonality: Monoclonal
Immunogen: PC12 cells
Clone:
Immunogen Sequence:
Myeloma Strain: NS-1
Epitope Mapped: Yes
Antigen Name: Integrin, alpha 1/ CD49a /VLA-1
Epitope Location or Sequence: Ser-Gly-Pro-Val-Glu-Asp-Met-Phe-Gly-Tyr-Thr-Val.
Alternate Antigen Name:
Deposit Date: 9/29/2015
Antigen Molecular Weight: 185 kDa /125 kDa
Depositor: Carbonetto, Salvatore / Turner, D.C.
Antigen Sequence:
Depositor Institution: McGill University
Antigen Species: Rat
Depositor Notes: mAB 3A3 promotes neurite retraction in PC12 cells and actively inhibits binding of several rat cell types to laminin and collagen. This antibody also labels focal contacts in rat astrocytes and fibroblasts.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Chick, Rat
Additional Information: mAB 3A3 inhibits interaction with the E1 laminin fragment (PMID: 2380249). RRID:AB_2722115
Recommended Applications: Function Blocking, Immunofluorescence, Immunohistochemistry, Immunoprecipitation
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
mAB 3A3 was deposited to the DSHB by Carbonetto, Salvatore / Turner, D.C. (DSHB Hybridoma Product mAB 3A3)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
12 References
Initial Publication
Identification of a cell-surface protein involved in PC12 cell-substratum adhesion and neurite outgrowth on laminin and collagen.
Carbonetto S
The Journal of neuroscience : the official journal of the Society for Neuroscience 9.9 (1989 Sep): 3287-96.