mAB 3A3

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$40.00
SKU: mAB 3A3
View product citations for antibody mAB 3A3 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: Integrin, alpha 1/ CD49a /VLA-1
Hybridoma Cells Available: Yes
Antigen Species: Rat
Depositor: Carbonetto, Salvatore / Turner, D.C.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: McGill University
Positive Tested Species Reactivity: Chick, Rat
Depositors Notes: mAB 3A3 promotes neurite retraction in PC12 cells and actively inhibits binding of several rat cell types to laminin and collagen. This antibody also labels focal contacts in rat astrocytes and fibroblasts.
Antigen Molecular Weight: 185 kDa /125 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: Itga1
Immunogen: PC12 cells
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: NS-1
Epitope Location or Sequence: Ser-Gly-Pro-Val-Glu-Asp-Met-Phe-Gly-Tyr-Thr-Val.
Uniprot ID: P18614 
Immunogen Sequence:
Entrez Gene ID: 25118 
Additional Characterization:  
Antibody Registry ID: AB_2722115 
Additional Information: mAB 3A3 inhibits interaction with the E1 laminin fragment (PMID: 2380249). RRID:AB_2722115
Recommended Applications: Function Blocking, Immunofluorescence, Immunohistochemistry, Immunoprecipitation
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
mAB 3A3 was deposited to the DSHB by Carbonetto, Salvatore / Turner, D.C. (DSHB Hybridoma Product mAB 3A3)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

12 References

  • Initial Publication
  • IF References
  • IHC References
  • IP References
  • FB References
  • Epitope Map References
  • All References
  • Initial Publication

    Identification of a cell-surface protein involved in PC12 cell-substratum adhesion and neurite outgrowth on laminin and collagen.
    Carbonetto S
    The Journal of neuroscience : the official journal of the Society for Neuroscience 9.9 (1989 Sep): 3287-96.

    IF References

    Alpha 1 beta 1 integrin heterodimer functions as a dual laminin/collagen receptor in neural cells.
    Carbonetto S
    Biochemistry 29.27 (1990 Jul 10): 6540-4.

    Integrins in point contacts mediate cell spreading: factors that regulate integrin accumulation in point contacts vs. focal contacts.
    Carbonetto S
    The Journal of cell biology 120.1 (1993 Jan): 261-71.

    A dual laminin/collagen receptor acts in peripheral nerve regeneration.
    David S
    Proceedings of the National Academy of Sciences of the United States of America 87.4 (1990 Feb): 1319-22.

    Cell-collagen adhesion is inhibited by monoclonal antibody 33.4 against the rat alpha 1-integrin subunit.
    Reutter W
    Experimental cell research 212.1 (1994 May): 155-60.

    Anti-beta 1 integrin antibody inhibits Schwann cell myelination.
    Bunge MB
    Journal of neurobiology 25.10 (1994 Oct): 1207-26.

    IHC References
    IP References

    Integrins in point contacts mediate cell spreading: factors that regulate integrin accumulation in point contacts vs. focal contacts.
    Carbonetto S
    The Journal of cell biology 120.1 (1993 Jan): 261-71.

    Identification of a cell-surface protein involved in PC12 cell-substratum adhesion and neurite outgrowth on laminin and collagen.
    Carbonetto S
    The Journal of neuroscience : the official journal of the Society for Neuroscience 9.9 (1989 Sep): 3287-96.

    Molecular cloning of the rat integrin alpha 1-subunit: a receptor for laminin and collagen.
    Reichardt LF
    The Journal of cell biology 111.2 (1990 Aug): 709-20.

    Expression of beta 1 integrins in sensory neurons of the dorsal root ganglion and their functions in neurite outgrowth on two laminin isoforms.
    Reichardt LF
    The Journal of neuroscience : the official journal of the Society for Neuroscience 13.11 (1993 Nov): 4880-8.

    An alpha 1/beta 1-like integrin receptor on rat aortic smooth muscle cells mediates adhesion to laminin and collagen types I and IV.
    Kramer RH
    Arteriosclerosis (Dallas, Tex.) 10.3 (1990 May-Jun): 402-9.

    FB References

    The extracellular matrix modulates the response of PC12 cells to nerve growth factor: cell aggregation versus neurite outgrowth on 3-dimensional laminin substrata.
    Carbonetto S
    Journal of neurocytology 18.4 (1989 Aug): 491-504.

    Identification of a cell-surface protein involved in PC12 cell-substratum adhesion and neurite outgrowth on laminin and collagen.
    Carbonetto S
    The Journal of neuroscience : the official journal of the Society for Neuroscience 9.9 (1989 Sep): 3287-96.

    A dual laminin/collagen receptor acts in peripheral nerve regeneration.
    David S
    Proceedings of the National Academy of Sciences of the United States of America 87.4 (1990 Feb): 1319-22.

    Alpha 1 beta 1 integrin heterodimer functions as a dual laminin/collagen receptor in neural cells.
    Carbonetto S
    Biochemistry 29.27 (1990 Jul 10): 6540-4.

    Integrins in point contacts mediate cell spreading: factors that regulate integrin accumulation in point contacts vs. focal contacts.
    Carbonetto S
    The Journal of cell biology 120.1 (1993 Jan): 261-71.

    Integrins and dystroglycan regulate astrocyte wound healing: the integrin beta1 subunit is necessary for process extension and orienting the microtubular network.
    Carbonetto S
    Developmental neurobiology 68.5 (2008 Apr): 559-74.

    Anti-beta 1 integrin antibody inhibits Schwann cell myelination.
    Bunge MB
    Journal of neurobiology 25.10 (1994 Oct): 1207-26.

    The alpha1beta1 integrin is expressed during neointima formation in rat arteries and mediates collagen matrix reorganization.
    Koteliansky VE
    The Journal of clinical investigation 97.11 (1996 Jun 1): 2469-77.

    An alpha 1/beta 1-like integrin receptor on rat aortic smooth muscle cells mediates adhesion to laminin and collagen types I and IV.
    Kramer RH
    Arteriosclerosis (Dallas, Tex.) 10.3 (1990 May-Jun): 402-9.

    Epitope Map References
    All References

    The alpha1beta1 integrin is expressed during neointima formation in rat arteries and mediates collagen matrix reorganization.
    Koteliansky VE
    The Journal of clinical investigation 97.11 (1996 Jun 1): 2469-77.

    Alpha 1 beta 1 integrin heterodimer functions as a dual laminin/collagen receptor in neural cells.
    Carbonetto S
    Biochemistry 29.27 (1990 Jul 10): 6540-4.

    Integrins in point contacts mediate cell spreading: factors that regulate integrin accumulation in point contacts vs. focal contacts.
    Carbonetto S
    The Journal of cell biology 120.1 (1993 Jan): 261-71.

    A dual laminin/collagen receptor acts in peripheral nerve regeneration.
    David S
    Proceedings of the National Academy of Sciences of the United States of America 87.4 (1990 Feb): 1319-22.

    Cell-collagen adhesion is inhibited by monoclonal antibody 33.4 against the rat alpha 1-integrin subunit.
    Reutter W
    Experimental cell research 212.1 (1994 May): 155-60.

    Anti-beta 1 integrin antibody inhibits Schwann cell myelination.
    Bunge MB
    Journal of neurobiology 25.10 (1994 Oct): 1207-26.

    Identification of a cell-surface protein involved in PC12 cell-substratum adhesion and neurite outgrowth on laminin and collagen.
    Carbonetto S
    The Journal of neuroscience : the official journal of the Society for Neuroscience 9.9 (1989 Sep): 3287-96.

    Molecular cloning of the rat integrin alpha 1-subunit: a receptor for laminin and collagen.
    Reichardt LF
    The Journal of cell biology 111.2 (1990 Aug): 709-20.

    Expression of beta 1 integrins in sensory neurons of the dorsal root ganglion and their functions in neurite outgrowth on two laminin isoforms.
    Reichardt LF
    The Journal of neuroscience : the official journal of the Society for Neuroscience 13.11 (1993 Nov): 4880-8.

    An alpha 1/beta 1-like integrin receptor on rat aortic smooth muscle cells mediates adhesion to laminin and collagen types I and IV.
    Kramer RH
    Arteriosclerosis (Dallas, Tex.) 10.3 (1990 May-Jun): 402-9.

    The extracellular matrix modulates the response of PC12 cells to nerve growth factor: cell aggregation versus neurite outgrowth on 3-dimensional laminin substrata.
    Carbonetto S
    Journal of neurocytology 18.4 (1989 Aug): 491-504.

    Integrins and dystroglycan regulate astrocyte wound healing: the integrin beta1 subunit is necessary for process extension and orienting the microtubular network.
    Carbonetto S
    Developmental neurobiology 68.5 (2008 Apr): 559-74.

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