mAb 20

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$40.00
SKU: mAb 20
View product citations for antibody mAb 20 on CiteAb

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Available: 14

DSHB Data Sheet

Catalog Fields

Antigen: zeugmatin
Hybridoma Cells Available: Yes
Antigen Species: chicken
Depositor: Singer, S.J. / Maher, P.
Isotype: MIgG2a
Antigen Sequence:
Host Species: mouse
Depositors Institution: University of California, San Diego
Positive Tested Species Reactivity: Chicken
Depositors Notes: Species specificity note: only chicken by immunofluorescence; weak reaction with mammals by immunoblotting. IHC note: antibody stains Z line in embryonic & adult skeletal & cardiac muscle; stains fascia adherens in cardiac muscle.
Antigen Molecular Weight: around 600 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene:
Immunogen: fascia adherens; chicken cardiac muscle
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: NS1
Epitope Location or Sequence:
Uniprot ID:  
Immunogen Sequence:
Entrez Gene ID:  
Additional Characterization:  
Antibody Registry ID: AB_531808 
Additional Information:
Recommended Applications: Immunofluorescence, Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
mAb 20 was deposited to the DSHB by Singer, S.J. / Maher, P. (DSHB Hybridoma Product mAb 20)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
All cell products contain the antimicrobial ProClin. Click here for additional information.

2 References

  • Initial Publication
  • IF References
  • All References
  • Initial Publication
    IF References

    Cell-type-specific, multicolor labeling of endogenous proteins with split fluorescent protein tags in <i>Drosophila</i>.
    Kamiyama D
    Proceedings of the National Academy of Sciences of the United States of America 118.23 (2021 Jun 8): .

    All References

    Cell-type-specific, multicolor labeling of endogenous proteins with split fluorescent protein tags in <i>Drosophila</i>.
    Kamiyama D
    Proceedings of the National Academy of Sciences of the United States of America 118.23 (2021 Jun 8): .

    Zeugmatin: a new high molecular weight protein associated with Z lines in adult and early embryonic striated muscle.
    Singer SJ
    The Journal of cell biology 101.5 Pt 1 (1985 Nov): 1871-83.

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