mAb 5-8

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$40.00
SKU: mAb 5-8
View product citations for antibody mAb 5-8 on CiteAb

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Available: 40

DSHB Data Sheet

Catalog Fields

Antigen: Unc-54; Myosin heavy chain-4 (B)
Hybridoma Cells Available: Yes
Antigen Species: C. elegans
Depositor: Miller, David, M., III
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: Vanderbilt University
Positive Tested Species Reactivity: C. elegans
Depositors Notes: This antigen is also known as myosin heavy chain B (MHC B) or as Uncoordinated protein 54.
Antigen Molecular Weight: 210 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: unc-54
Immunogen: Purified nematode myosin
Alternate Gene Names: myo-4,CELE_F11C3.3
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: S194/5.XXO.Bu.1
Epitope Location or Sequence: C-terminal domain
Uniprot ID: P02566 
Immunogen Sequence:
Entrez Gene ID: 259839 
Additional Characterization:  
Antibody Registry ID: AB_2801351 
Additional Information: WormBase:WBGene00006789
Recommended Applications: ELISA, Immunofluorescence, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
mAb 5-8 was deposited to the DSHB by Miller, David, M., III (DSHB Hybridoma Product mAb 5-8)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

6 References

  • Initial Publication
  • IF References
  • WB References
  • ELISA References
  • Epitope Map References
  • All References
  • Initial Publication
    IF References
    WB References
    ELISA References
    Epitope Map References

    Immunological identification of the genes encoding the four myosin heavy chain isoforms of Caenorhabditis elegans.
    Karn J
    Proceedings of the National Academy of Sciences of the United States of America 83.8 (1986 Apr): 2305-9.

    All References

    Unc-45 mutations in Caenorhabditis elegans implicate a CRO1/She4p-like domain in myosin assembly.
    Epstein HF
    The Journal of cell biology 143.5 (1998 Nov 30): 1215-25.

    Case history: cancer of the breast.
    Brus H
    The Lamp 36.11 (1979 Dec): 26-9.

    Myosin and paramyosin of Caenorhabditis elegans embryos assemble into nascent structures distinct from thick filaments and multi-filament assemblages.
    Ortiz I
    The Journal of cell biology 122.4 (1993 Aug): 845-58.

    Differential localization of two myosins within nematode thick filaments.
    Epstein HF
    Cell 34.2 (1983 Sep): 477-90.

    The UNC-45 chaperone mediates sarcomere assembly through myosin degradation in Caenorhabditis elegans.
    Epstein HF
    The Journal of cell biology 177.2 (2007 Apr 23): 205-10.

    Immunological identification of the genes encoding the four myosin heavy chain isoforms of Caenorhabditis elegans.
    Karn J
    Proceedings of the National Academy of Sciences of the United States of America 83.8 (1986 Apr): 2305-9.

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