mAb 5A6

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SKU: mAb 5A6

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DSHB Data Sheet

Catalog Fields

Clone ID/Product Name: mAb 5A6
Available to For-Profits: No
Alternate Antibody Name: 5A6
Gene Symbol: K-CAM
Ab Isotype: MIgG1
Gene Name:
Antibody Registry ID: AB_528112 
Uniprot ID: P33145 
RRID:  
Entrez Gene ID: 414845 
Clonality: Monoclonal
Immunogen: L929 mouse cells expressing recombinant chick B-cadherin
Clone:
Immunogen Sequence: Full length protein
Myeloma Strain: SP2/0
Epitope Mapped: Yes
Antigen Name: B-cadherin (cadherin-3)
Epitope Location or Sequence: extracellular domain (see depositor notes)
Alternate Antigen Name:
Deposit Date: 4/20/1999
Antigen Molecular Weight: Predicted: 80 kDa; Apparent: 120 kDa
Depositor: Reichardt, L.
Antigen Sequence:
Depositor Institution: University of California, San Francisco
Antigen Species: chicken
Depositor Notes: mAb 5A6 doesn't work on SDS-denatured B-cadherin. It recognizes the extracellular domain.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Chicken
Additional Information: Not available to for-profit organizations. For further information please contact the Office of Technology Management and Advancement at the University of California. Refer to case number 95-082. Email : innovation@ucsf.edu

B-cadherin is also known as cadherin 3.
Predicted Species Reactivity:  
Human Protein Atlas:  
Additional Characterization:  
Recommended Applications: ELISA, Function Blocking, Immunofluorescence, Immunoprecipitation
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
mAb 5A6 was deposited to the DSHB by Reichardt, L. (DSHB Hybridoma Product mAb 5A6)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

6 References

  • Initial Publication
  • IF References
  • IP References
  • ELISA References
  • FB References
  • All References
  • Initial Publication

    The expression of B-cadherin during embryonic chick development.
    Reichardt LF
    Developmental biology 161.1 (1994 Jan): 107-25.

    IF References

    The expression of B-cadherin during embryonic chick development.
    Reichardt LF
    Developmental biology 161.1 (1994 Jan): 107-25.

    The cadherin-binding specificities of B-cadherin and LCAM.
    Reichardt LF
    The Journal of cell biology 129.5 (1995 Jun): 1379-90.

    Cadherin-mediated cell adhesion and tissue segregation: qualitative and quantitative determinants.
    Steinberg MS
    Developmental biology 253.2 (2003 Jan 15): 309-23.

    Role of apical and basolateral membranes in replication of human cytomegalovirus in polarized retinal pigment epithelial cells.
    Pereira L
    The Journal of general virology 77 ( Pt 1). (1996 Jan): 61-74.

    IP References

    The expression of B-cadherin during embryonic chick development.
    Reichardt LF
    Developmental biology 161.1 (1994 Jan): 107-25.

    ELISA References

    The expression of B-cadherin during embryonic chick development.
    Reichardt LF
    Developmental biology 161.1 (1994 Jan): 107-25.

    FB References

    The cadherin-binding specificities of B-cadherin and LCAM.
    Reichardt LF
    The Journal of cell biology 129.5 (1995 Jun): 1379-90.

    Cadherin-mediated cell adhesion and tissue segregation: qualitative and quantitative determinants.
    Steinberg MS
    Developmental biology 253.2 (2003 Jan 15): 309-23.

    All References

    The expression of B-cadherin during embryonic chick development.
    Reichardt LF
    Developmental biology 161.1 (1994 Jan): 107-25.

    The cadherin-binding specificities of B-cadherin and LCAM.
    Reichardt LF
    The Journal of cell biology 129.5 (1995 Jun): 1379-90.

    Cadherin-mediated cell adhesion and tissue segregation: qualitative and quantitative determinants.
    Steinberg MS
    Developmental biology 253.2 (2003 Jan 15): 309-23.

    Role of apical and basolateral membranes in replication of human cytomegalovirus in polarized retinal pigment epithelial cells.
    Pereira L
    The Journal of general virology 77 ( Pt 1). (1996 Jan): 61-74.

    The brain chondroitin sulfate proteoglycan brevican associates with astrocytes ensheathing cerebellar glomeruli and inhibits neurite outgrowth from granule neurons.
    Yamaguchi Y
    The Journal of neuroscience : the official journal of the Society for Neuroscience 17.20 (1997 Oct 15): 7784-95.

    Similarities in structure and expression between mouse P-cadherin, chicken B-cadherin and frog XB/U-cadherin.
    Müller HA
    Cell adhesion and communication 2.6 (1994 Dec): 511-20.

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