mAb 5A6
Catalog Fields
Product Name/ID: mAb 5A6
Available to For-Profits: No
Alternate Antibody Name: 5A6
Gene Name: K-CAM
Ab Isotype: MIgG1
Gene Symbol:
Entrez Gene ID: 414845
Clonality: Monoclonal
Immunogen: L929 mouse cells expressing recombinant chick B-cadherin
Clone:
Immunogen Sequence: Full length protein
Myeloma Strain: SP2/0
Epitope Mapped: Yes
Antigen Name: B-cadherin (cadherin-3)
Epitope Location or Sequence: extracellular domain (see depositor notes)
Alternate Antigen Name:
Deposit Date: 4/20/1999
Antigen Molecular Weight: Predicted: 80 kDa; Apparent: 120 kDa
Depositor: Reichardt, L.
Antigen Sequence:
Depositor Institution: University of California, San Francisco
Antigen Species: chicken
Depositor Notes: mAb 5A6 doesn't work on SDS-denatured B-cadherin. It recognizes the extracellular domain.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Chicken
Additional Information: Not available to for-profit organizations. For further information please contact the Office of Innovation, Technology, and Alliances at the University of California. Refer to case number 95-082. Email : ita@ucsf.edu Telephone: 415 502-1603.
B-cadherin is also known as cadherin 3.
B-cadherin is also known as cadherin 3.
Recommended Applications: ELISA, Function Blocking, Immunofluorescence, Immunoprecipitation
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
mAb 5A6 was deposited to the DSHB by Reichardt, L. (DSHB Hybridoma Product mAb 5A6)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
6 References
Initial Publication
The expression of B-cadherin during embryonic chick development.
Reichardt LF
Developmental biology 161.1 (1994 Jan): 107-25.