mAb 5A6

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$40.00
SKU: mAb 5A6
View product citations for antibody mAb 5A6 on CiteAb

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Available: 40

DSHB Data Sheet

Catalog Fields

Antigen: B-cadherin (cadherin-3)
Hybridoma Cells Available: Yes
Antigen Species: chicken
Depositor: Reichardt, L.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: Department of Physiology, University of California
Positive Tested Species Reactivity: Chicken
Depositors Notes: mAb 5A6 doesn't work on SDS-denatured B-cadherin. It recognizes the extracellular domain.
Antigen Molecular Weight: Predicted: 80 kDa; Apparent: 120 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: K-CAM
Immunogen: L929 mouse cells expressing recombinant chick B-cadherin
Alternate Gene Names: CDH3
Alternate Antibody Name: 5A6
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: SP2/0
Epitope Location or Sequence: extracellular domain (see depositor notes)
Uniprot ID: P33145 
Immunogen Sequence: Full length protein
Entrez Gene ID: 414845 
Additional Characterization:  
Antibody Registry ID: AB_528112 
Additional Information: B-cadherin is also known as cadherin 3.
Recommended Applications: ELISA, Function Blocking, Immunofluorescence, Immunoprecipitation
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
mAb 5A6 was deposited to the DSHB by Reichardt, L. (DSHB Hybridoma Product mAb 5A6)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

6 References

  • Initial Publication
  • IF References
  • IP References
  • ELISA References
  • FB References
  • All References
  • Initial Publication

    The expression of B-cadherin during embryonic chick development.
    Reichardt LF
    Developmental biology 161.1 (1994 Jan): 107-25.

    IF References

    The expression of B-cadherin during embryonic chick development.
    Reichardt LF
    Developmental biology 161.1 (1994 Jan): 107-25.

    The cadherin-binding specificities of B-cadherin and LCAM.
    Reichardt LF
    The Journal of cell biology 129.5 (1995 Jun): 1379-90.

    Cadherin-mediated cell adhesion and tissue segregation: qualitative and quantitative determinants.
    Steinberg MS
    Developmental biology 253.2 (2003 Jan 15): 309-23.

    Role of apical and basolateral membranes in replication of human cytomegalovirus in polarized retinal pigment epithelial cells.
    Pereira L
    The Journal of general virology 77 ( Pt 1). (1996 Jan): 61-74.

    IP References

    The expression of B-cadherin during embryonic chick development.
    Reichardt LF
    Developmental biology 161.1 (1994 Jan): 107-25.

    ELISA References

    The expression of B-cadherin during embryonic chick development.
    Reichardt LF
    Developmental biology 161.1 (1994 Jan): 107-25.

    FB References

    The cadherin-binding specificities of B-cadherin and LCAM.
    Reichardt LF
    The Journal of cell biology 129.5 (1995 Jun): 1379-90.

    Cadherin-mediated cell adhesion and tissue segregation: qualitative and quantitative determinants.
    Steinberg MS
    Developmental biology 253.2 (2003 Jan 15): 309-23.

    All References

    The expression of B-cadherin during embryonic chick development.
    Reichardt LF
    Developmental biology 161.1 (1994 Jan): 107-25.

    The cadherin-binding specificities of B-cadherin and LCAM.
    Reichardt LF
    The Journal of cell biology 129.5 (1995 Jun): 1379-90.

    Cadherin-mediated cell adhesion and tissue segregation: qualitative and quantitative determinants.
    Steinberg MS
    Developmental biology 253.2 (2003 Jan 15): 309-23.

    Role of apical and basolateral membranes in replication of human cytomegalovirus in polarized retinal pigment epithelial cells.
    Pereira L
    The Journal of general virology 77 ( Pt 1). (1996 Jan): 61-74.

    The brain chondroitin sulfate proteoglycan brevican associates with astrocytes ensheathing cerebellar glomeruli and inhibits neurite outgrowth from granule neurons.
    Yamaguchi Y
    The Journal of neuroscience : the official journal of the Society for Neuroscience 17.20 (1997 Oct 15): 7784-95.

    Similarities in structure and expression between mouse P-cadherin, chicken B-cadherin and frog XB/U-cadherin.
    Müller HA
    Cell adhesion and communication 2.6 (1994 Dec): 511-20.

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