neuroglian 3B11

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$40.00
SKU: neuroglian 3B11
View product citations for antibody neuroglian 3B11 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: neuroglian (Manduca sexta)
Hybridoma Cells Available: Yes
Antigen Species: Manduca sexta
Depositor: Nardi, J.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: Department of Entomology, University of Illinois
Positive Tested Species Reactivity: Manduca sexta
Depositors Notes:
Antigen Molecular Weight: Predicted: 140 kDa; Apparent: 156 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: Nrg
Immunogen: Developing adult (four days after pupation) wing epithelia membrane preparartion.
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: Sp2/0
Epitope Location or Sequence: aa 18-605 (extracellular domain encompassing the six Ig domains)
Uniprot ID: P91767 
Immunogen Sequence: Total protein
Entrez Gene ID:  
Additional Characterization:  
Antibody Registry ID: AB_10804674 
Additional Information: Recognizes the extracellular region (encompassing the six Ig domains) of neuroglian. Works on western blots under nonreducing conditions. The initial publication for this antibody is: Nardi, J.B. (1993). Modulated expression of a surface epitope on migrating germ cells of Manduca sexta embryos. Development 118, 967-975.
Recommended Applications: Function Blocking, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
neuroglian 3B11 was deposited to the DSHB by Nardi, J. (DSHB Hybridoma Product neuroglian 3B11)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

10 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • IP References
  • FB References
  • Epitope Map References
  • All References
  • Initial Publication

    Rearrangement of epithelial cell types in an insect wing monolayer is accompanied by differential expression of a cell surface protein.
    Nardi JB
    Developmental dynamics : an official publication of the American Association of Anatomists 199.4 (1994 Apr): 315-25.

    IF References
    WB References
    IHC References

    Rearrangement of epithelial cell types in an insect wing monolayer is accompanied by differential expression of a cell surface protein.
    Nardi JB
    Developmental dynamics : an official publication of the American Association of Anatomists 199.4 (1994 Apr): 315-25.

    Neuroglian is expressed on cells destined to form the prothoracic glands of Manduca embryos as they segregate from surrounding cells and rearrange during morphogenesis.
    Nardi JB
    Developmental biology 181.1 (1997 Jan 1): 1-13.

    Hematopoietic organs of Manduca sexta and hemocyte lineages.
    Kanost MR
    Development genes and evolution 213.10 (2003 Oct): 477-91.

    IP References

    Rearrangement of epithelial cell types in an insect wing monolayer is accompanied by differential expression of a cell surface protein.
    Nardi JB
    Developmental dynamics : an official publication of the American Association of Anatomists 199.4 (1994 Apr): 315-25.

    FB References
    Epitope Map References
    All References

    Rearrangement of epithelial cell types in an insect wing monolayer is accompanied by differential expression of a cell surface protein.
    Nardi JB
    Developmental dynamics : an official publication of the American Association of Anatomists 199.4 (1994 Apr): 315-25.

    Neuroglian is expressed on cells destined to form the prothoracic glands of Manduca embryos as they segregate from surrounding cells and rearrange during morphogenesis.
    Nardi JB
    Developmental biology 181.1 (1997 Jan 1): 1-13.

    Hematopoietic organs of Manduca sexta and hemocyte lineages.
    Kanost MR
    Development genes and evolution 213.10 (2003 Oct): 477-91.

    Neuroglian-positive plasmatocytes of Manduca sexta and the initiation of hemocyte attachment to foreign surfaces.
    Kanost MR
    Developmental and comparative immunology 30.5 (2006): 447-62.

    Activation of epidermal growth factor receptor mediates receptor axon sorting and extension in the developing olfactory system of the moth Manduca sexta.
    Tolbert LP
    The Journal of comparative neurology 495.5 (2006 Apr 10): 554-72.

    Roles of specific membrane lipid domains in EGF receptor activation and cell adhesion molecule stabilization in a developing olfactory system.
    Oland LA
    PloS one 4.9 (2009 Sep 29): e7222.

    Clustering of adhesion receptors following exposure of insect blood cells to foreign surfaces.
    Kanost MR
    Journal of insect physiology 51.5 (2005 May): 555-64.

    Reverse signaling by glycosylphosphatidylinositol-linked Manduca ephrin requires a SRC family kinase to restrict neuronal migration in vivo.
    Copenhaver PF
    The Journal of neuroscience : the official journal of the Society for Neuroscience 29.11 (2009 Mar 18): 3404-18.

    Neuroglian on hemocyte surfaces is involved in homophilic and heterophilic interactions of the innate immune system of Manduca sexta.
    Kanost MR
    Developmental and comparative immunology 31.11 (2007): 1159-67.

    Multiple alpha subunits of integrin are involved in cell-mediated responses of the Manduca immune system.
    Kanost MR
    Developmental and comparative immunology 32.4 (2008): 365-79.

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