neuroglian 3B11
Catalog Fields
Clone ID/Product Name: neuroglian 3B11
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Symbol: Nrg
Ab Isotype: MIgG1
Gene Name:
Antibody Registry ID: AB_10804674
Uniprot ID: P91767
Clonality: Monoclonal
Immunogen: Developing adult (four days after pupation) wing epithelia membrane preparartion.
Clone:
Immunogen Sequence: Total protein
Myeloma Strain: Sp2/0
Epitope Mapped: Yes
Antigen Name: neuroglian (Manduca sexta)
Epitope Location or Sequence: aa 18-605 (extracellular domain encompassing the six Ig domains)
Alternate Antigen Name:
Deposit Date: 1/3/2011
Antigen Molecular Weight: Predicted: 140 kDa; Apparent: 156 kDa
Depositor: Nardi, J.
Antigen Sequence:
Depositor Institution: University of Illinois
Antigen Species: Manduca sexta
Depositor Notes:
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Manduca sexta
Additional Information: Recognizes the extracellular region (encompassing the six Ig domains) of neuroglian. Works on western blots under nonreducing conditions. The initial publication for this antibody is: Nardi, J.B. (1993). Modulated expression of a surface epitope on migrating germ cells of Manduca sexta embryos. Development 118, 967-975.
Recommended Applications: Function Blocking, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
neuroglian 3B11 was deposited to the DSHB by Nardi, J. (DSHB Hybridoma Product neuroglian 3B11)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
10 References
Initial Publication
Rearrangement of epithelial cell types in an insect wing monolayer is accompanied by differential expression of a cell surface protein.
Nardi JB
Developmental dynamics : an official publication of the American Association of Anatomists 199.4 (1994 Apr): 315-25.