neuroglian 3B11

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$40.00
SKU: neuroglian 3B11
View product citations for antibody neuroglian 3B11 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: neuroglian (Manduca sexta)
Hybridoma Cells Available: Yes
Antigen Species: Manduca sexta
Depositor: Nardi, J.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: Department of Entomology, University of Illinois
Positive Tested Species Reactivity: Manduca sexta
Depositors Notes:
Antigen Molecular Weight: Predicted: 140 kDa; Apparent: 156 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: Nrg
Immunogen: Developing adult (four days after pupation) wing epithelia membrane preparartion.
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: Sp2/0
Epitope Location or Sequence: aa 18-605 (extracellular domain encompassing the six Ig domains)
Uniprot ID: P91767 
Immunogen Sequence: Total protein
Entrez Gene ID:  
Additional Characterization:  
Antibody Registry ID: AB_10804674 
Additional Information: Recognizes the extracellular region (encompassing the six Ig domains) of neuroglian. Works on western blots under nonreducing conditions. The initial publication for this antibody is: Nardi, J.B. (1993). Modulated expression of a surface epitope on migrating germ cells of Manduca sexta embryos. Development 118, 967-975.
Recommended Applications: Function Blocking, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
neuroglian 3B11 was deposited to the DSHB by Nardi, J. (DSHB Hybridoma Product neuroglian 3B11)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

10 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • IP References
  • FB References
  • Epitope Map References
  • All References
  • Initial Publication
    IF References
    WB References
    IHC References
    IP References
    FB References
    Epitope Map References
    All References

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