opa 4B12

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$40.00
SKU: opa 4B12
View product citations for antibody opa 4B12 on CiteAb

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Available: 10

DSHB Data Sheet

Catalog Fields

Antigen: Opa outer membrane protein/ opacity protein
Hybridoma Cells Available: Yes
Antigen Species: Neisseria gonorrhoeae
Depositor: Criss, A.
Isotype: MIgG2a
Antigen Sequence:
Host Species: mouse
Depositors Institution: University of Virginia
Positive Tested Species Reactivity:  
Depositors Notes:
Antigen Molecular Weight: 26.5 kD
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: opa A
Immunogen: Purified P.II protein from N. gonorrhoea R10
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: P3-X63-Ag 8 .6 .5 .3
Epitope Location or Sequence: a.a. 7-57
Uniprot ID:  
Immunogen Sequence: Full length sequence
Entrez Gene ID:  
Additional Characterization:  
Antibody Registry ID:  
Additional Information:
Recommended Applications: ELISA
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
opa 4B12 was deposited to the DSHB by Criss, A. (DSHB Hybridoma Product opa 4B12)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

19 References

  • Initial Publication
  • IF References
  • WB References
  • ELISA References
  • FACS References
  • Epitope Map References
  • All References
  • Initial Publication
    IF References
    WB References

    Purification and characterization of eight class 5 outer membrane protein variants from a clone of Neisseria meningitidis serogroup A.
    Moll A
    The Journal of experimental medicine 168.2 (1988 Aug 1): 507-25.

    The opacity proteins of Neisseria gonorrhoeae strain MS11 are encoded by a family of 11 complete genes.
    Swanson J
    Molecular microbiology 5.8 (1991 Aug): 1889-901.

    A comparison of the variable antigens expressed by clone IV-1 and subgroup III of Neisseria meningitidis serogroup A.
    Zollinger W
    The Journal of infectious diseases 165.1 (1992 Jan): 53-68.

    Variation in phenotypic expression of the Opa outer membrane protein and lipooligosaccharide of Neisseria meningitidis serogroup C causing periorbital cellulitis and bacteremia.
    Baker CJ
    Clinical infectious diseases : an official publication of the Infectious Diseases Society of America 16.4 (1993 Apr): 523-7.

    Heparin protects Opa+ Neisseria gonorrhoeae from the bactericidal action of normal human serum.
    Belland RJ
    Infection and immunity 63.5 (1995 May): 1790-5.

    Human experimentation with Neisseria gonorrhoeae: rationale, methods, and implications for the biology of infection and vaccine development.
    Whicker LG
    The Journal of infectious diseases 169.3 (1994 Mar): 532-7.

    Antigenic and epidemiologic properties of the ET-37 complex of Neisseria meningitidis.
    Achtman M
    The Journal of infectious diseases 167.6 (1993 Jun): 1320-9.

    Binding of syndecan-like cell surface proteoglycan receptors is required for Neisseria gonorrhoeae entry into human mucosal cells.
    Paul SM
    The EMBO journal 14.10 (1995 May 15): 2144-54.

    Adherence of pilus- Opa+ gonococci to epithelial cells in vitro involves heparan sulfate.
    Swanson J
    The Journal of experimental medicine 182.2 (1995 Aug 1): 511-7.

    Neisserial Opa Protein-CEACAM Interactions: Competition for Receptors as a Means of Bacterial Invasion and Pathogenesis.
    Columbus L
    Biochemistry 55.31 (2016 Aug 9): 4286-94.

    Sterilizing immunity elicited by Neisseria meningitidis carriage shows broader protection than predicted by serum antibody cross-reactivity in CEACAM1-humanized mice.
    Gray-Owen SD
    Infection and immunity 83.1 (2015 Jan): 354-63.

    Role of lipooligosaccharide in Opa-independent invasion of Neisseria gonorrhoeae into human epithelial cells.
    Stein DC
    The Journal of experimental medicine 191.6 (2000 Mar 20): 949-60.

    Carcinoembryonic antigen family receptor specificity of Neisseria meningitidis Opa variants influences adherence to and invasion of proinflammatory cytokine-activated endothelial cells.
    Gray-Owen SD
    Infection and immunity 68.6 (2000 Jun): 3601-7.

    Constitutively Opa-expressing and Opa-deficient neisseria gonorrhoeae strains differentially stimulate and survive exposure to human neutrophils.
    Criss AK
    Journal of bacteriology 195.13 (2013 Jul): 2982-90.

    Construction and characterization of a derivative of Neisseria gonorrhoeae strain MS11 devoid of all opa genes.
    Stein DC
    Journal of bacteriology 194.23 (2012 Dec): 6468-78.

    Mannose-binding lectin binds to two major outer membrane proteins, opacity protein and porin, of Neisseria meningitidis.
    Jarvis GA
    Journal of immunology (Baltimore, Md. : 1950) 172.6 (2004 Mar 15): 3784-92.

    Lipooligosaccharide-deficient Neisseria meningitidis shows altered pilus-associated characteristics.
    Jonsson AB
    Infection and immunity 71.1 (2003 Jan): 155-62.

    ELISA References
    FACS References
    Epitope Map References
    All References

    Construction and characterization of a derivative of Neisseria gonorrhoeae strain MS11 devoid of all opa genes.
    Stein DC
    Journal of bacteriology 194.23 (2012 Dec): 6468-78.

    Purification and characterization of eight class 5 outer membrane protein variants from a clone of Neisseria meningitidis serogroup A.
    Moll A
    The Journal of experimental medicine 168.2 (1988 Aug 1): 507-25.

    The opacity proteins of Neisseria gonorrhoeae strain MS11 are encoded by a family of 11 complete genes.
    Swanson J
    Molecular microbiology 5.8 (1991 Aug): 1889-901.

    A comparison of the variable antigens expressed by clone IV-1 and subgroup III of Neisseria meningitidis serogroup A.
    Zollinger W
    The Journal of infectious diseases 165.1 (1992 Jan): 53-68.

    Variation in phenotypic expression of the Opa outer membrane protein and lipooligosaccharide of Neisseria meningitidis serogroup C causing periorbital cellulitis and bacteremia.
    Baker CJ
    Clinical infectious diseases : an official publication of the Infectious Diseases Society of America 16.4 (1993 Apr): 523-7.

    Heparin protects Opa+ Neisseria gonorrhoeae from the bactericidal action of normal human serum.
    Belland RJ
    Infection and immunity 63.5 (1995 May): 1790-5.

    Human experimentation with Neisseria gonorrhoeae: rationale, methods, and implications for the biology of infection and vaccine development.
    Whicker LG
    The Journal of infectious diseases 169.3 (1994 Mar): 532-7.

    Antigenic and epidemiologic properties of the ET-37 complex of Neisseria meningitidis.
    Achtman M
    The Journal of infectious diseases 167.6 (1993 Jun): 1320-9.

    Binding of syndecan-like cell surface proteoglycan receptors is required for Neisseria gonorrhoeae entry into human mucosal cells.
    Paul SM
    The EMBO journal 14.10 (1995 May 15): 2144-54.

    Adherence of pilus- Opa+ gonococci to epithelial cells in vitro involves heparan sulfate.
    Swanson J
    The Journal of experimental medicine 182.2 (1995 Aug 1): 511-7.

    Neisserial Opa Protein-CEACAM Interactions: Competition for Receptors as a Means of Bacterial Invasion and Pathogenesis.
    Columbus L
    Biochemistry 55.31 (2016 Aug 9): 4286-94.

    Sterilizing immunity elicited by Neisseria meningitidis carriage shows broader protection than predicted by serum antibody cross-reactivity in CEACAM1-humanized mice.
    Gray-Owen SD
    Infection and immunity 83.1 (2015 Jan): 354-63.

    Role of lipooligosaccharide in Opa-independent invasion of Neisseria gonorrhoeae into human epithelial cells.
    Stein DC
    The Journal of experimental medicine 191.6 (2000 Mar 20): 949-60.

    Carcinoembryonic antigen family receptor specificity of Neisseria meningitidis Opa variants influences adherence to and invasion of proinflammatory cytokine-activated endothelial cells.
    Gray-Owen SD
    Infection and immunity 68.6 (2000 Jun): 3601-7.

    Constitutively Opa-expressing and Opa-deficient neisseria gonorrhoeae strains differentially stimulate and survive exposure to human neutrophils.
    Criss AK
    Journal of bacteriology 195.13 (2013 Jul): 2982-90.

    Mannose-binding lectin binds to two major outer membrane proteins, opacity protein and porin, of Neisseria meningitidis.
    Jarvis GA
    Journal of immunology (Baltimore, Md. : 1950) 172.6 (2004 Mar 15): 3784-92.

    Lipooligosaccharide-deficient Neisseria meningitidis shows altered pilus-associated characteristics.
    Jonsson AB
    Infection and immunity 71.1 (2003 Jan): 155-62.

    Gonococcal opacity: lectin-like interactions between Opa proteins and lipooligosaccharide.
    Mandrell RE
    Infection and immunity 63.4 (1995 Apr): 1434-9.

    Expression and phase variation of gonococcal P.II genes in Escherichia coli involves ribosomal frameshifting and slipped-strand mispairing.
    Swanson J
    Molecular microbiology 3.6 (1989 Jun): 777-86.

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