opa 4B12
Catalog Fields
Antigen: Opa outer membrane protein/ opacity protein
Hybridoma Cells Available: Yes
Antigen Species: Neisseria gonorrhoeae
Depositor: Criss, A.
Isotype: MIgG2a
Antigen Sequence:
Host Species: mouse
Depositors Institution: University of Virginia
Positive Tested Species Reactivity:
Depositors Notes:
Predicted Species Reactivity:
Gene: opa A
Immunogen: Purified P.II protein from N. gonorrhoea R10
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: P3-X63-Ag 8 .6 .5 .3
Epitope Location or Sequence: a.a. 7-57
Antibody Registry ID: AB_2889811
Additional Information: RRID: AB_2889811
Recommended Applications: ELISA
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
opa 4B12 was deposited to the DSHB by Criss, A. (DSHB Hybridoma Product opa 4B12)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
All cell products contain the antimicrobial ProClin. Click here for additional information.
19 References
Initial Publication
Purification and characterization of eight class 5 outer membrane protein variants from a clone of Neisseria meningitidis serogroup A.
Moll A
The Journal of experimental medicine 168.2 (1988 Aug 1): 507-25.