poly(GP) TALS 828.179

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SKU: poly(GP) TALS 828.179
View product citations for antibody poly(GP) TALS 828.179 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: GP dipeptide repeat proteins, poly(GP)
Available to For-Profits: Yes
Antigen Species: Human
Hybridoma Cells Available (Non-Profit): Yes
Isotype: MIgG1
Depositor: Target ALS Foundation
Host Species: mouse
Antigen Sequence:
Positive Tested Species Reactivity: Human
Depositors Institution: Target ALS Foundation
Antigen Molecular Weight: The molecular weight will depend on the number of GP dipeptide repeats making up the protein.
Depositors Notes: This antibody recognizes poly(GP) dipeptide repeat proteins produced from an expanded hexanucleotide (GGGGCC) repeat in the first intron of C9ORF72, the most common known genetic cause of amyotrophic lateral sclerosis (ALS) and frontotemporal dementia (FTD). The expanded GGGGCC repeat undergoes an atypical form of translation - repeat-associated non-ATG (RAN) translation - forming various dipeptide repeat proteins, one of which is poly(GP). The hybridoma was commercially generated with funds from the Target ALS Foundation.
Predicted Species Reactivity:  
Human Protein Atlas:  
Immunogen: H2N-C(DPEG4) GPGPGPGPGPGPGPGP
Gene: C9ORF72
Alternate Antibody Name:
Alternate Gene Names:
Alternate Antigen Name:
Clonality: Monoclonal
Myeloma Strain:
Epitope Mapped: No
Uniprot ID: Q96LT7 
Epitope Location or Sequence:
Entrez Gene ID: 203228 
Immunogen Sequence: GPGPGPGPGPGPGPGP
Antibody Registry ID: AB_2753317 
Additional Characterization:  
Recommended Applications: Immunohistochemistry, Western Blot
Additional Information:
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
poly(GP) TALS 828.179 was deposited to the DSHB by Target ALS Foundation (DSHB Hybridoma Product poly(GP) TALS 828.179)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

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