Antigen: GP dipeptide repeat proteins, poly(GP)
Hybridoma Cells Available: Yes
Antigen Species: Human
Depositor: Target ALS Foundation
Host Species: mouse
Depositors Institution: Target ALS Foundation
Positive Tested Species Reactivity: Human
Depositors Notes: This antibody recognizes poly(GP) dipeptide repeat proteins produced from an expanded hexanucleotide (GGGGCC) repeat in the first intron of C9ORF72, the most common known genetic cause of amyotrophic lateral sclerosis (ALS) and frontotemporal dementia (FTD). The expanded GGGGCC repeat undergoes an atypical form of translation - repeat-associated non-ATG (RAN) translation - forming various dipeptide repeat proteins, one of which is poly(GP). The hybridoma was commercially generated with funds from the Target ALS Foundation.
Antigen Molecular Weight: The molecular weight will depend on the number of GP dipeptide repeats making up the protein.
Predicted Species Reactivity:
Immunogen: H2N-C(DPEG4) GPGPGPGPGPGPGPGP
Alternate Gene Names:
Alternate Antibody Name:
Alternate Antigen Name:
Epitope Mapped: No
Epitope Location or Sequence:
Uniprot ID: Q96LT7
Immunogen Sequence: GPGPGPGPGPGPGPGP
Entrez Gene ID: 203228
Recommended Applications: Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
poly(GP) TALS 828.179 was deposited to the DSHB by Target ALS Foundation (DSHB Hybridoma Product poly(GP) TALS 828.179)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.