CT3-s

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DSHB Data Sheet
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Catalog Fields

Clone ID/Product Name: CT3-s
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Symbol: TNNT2
Ab Isotype: MIgG2a
Gene Name:
Antibody Registry ID: AB_528495 
Uniprot ID: P13789 
RRID:  
Entrez Gene ID: 286816 
Clonality: Monoclonal
Immunogen: Purified cardiac troponin T
Clone:
Immunogen Sequence: Full length protein
Myeloma Strain: NS1
Epitope Mapped: Yes
Antigen Name: troponin T (cardiac/slow)
Epitope Location or Sequence: aa 94-178
Alternate Antigen Name:
Deposit Date: 1/2/1996
Antigen Molecular Weight: Predicted (cTnT): 34 kDa; Apparent (cTnT): 43kDa
Depositor: Lin, J.J.-C.
Antigen Sequence:
Depositor Institution: University of Iowa
Antigen Species: Bovine
Depositor Notes: CT3 was initially published in: Jin, J., Lin, J.-C., and Lin, J.J.-C. (1990). Troponin T isoform switching during heart development. Ann. NY Acad. Sci. 588, 393-396.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Broad species, Chicken, Fish, Human, Mouse, Rabbit, Rat, Xenopus
Additional Information: CT3 recognizes the embryonic and adult cardiac isforms [PMID: 2358124]. CT3 cross-react with slow skeletal muscle TnT but doesn't recognize fast skeletal muscle TnT [PMID: 12732643].
Predicted Species Reactivity:  
Human Protein Atlas:  
Additional Characterization:  
Recommended Applications: FFPE, Immunofluorescence, Immunohistochemistry, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
CT3-s was deposited to the DSHB by Lin, J.J.-C. (DSHB Hybridoma Product CT3-s)
Storage and Handling Recommendations
Usage Recommendations

16 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • FACS References
  • Epitope Map References
  • All References
    • Initial Publication

    Precocious appearance of cardiac troponin T pre-mRNAs during early avian embryonic skeletal muscle development in ovo.
    Solursh M
    Developmental biology 140.1 (1990 Jul): 73-82.

    IF References

    Precocious appearance of cardiac troponin T pre-mRNAs during early avian embryonic skeletal muscle development in ovo.
    Solursh M
    Developmental biology 140.1 (1990 Jul): 73-82.

    Forced expression and assembly of rat cardiac troponin T isoforms in cultured muscle and nonmuscle cells.
    Lin JJ
    Journal of muscle research and cell motility 14.6 (1993 Dec): 619-32.

    Confocal imaging of early heart development in Xenopus laevis.
    Weeks DL
    Developmental biology 218.1 (2000 Feb 1): 64-73.

    Pitx2c attenuation results in cardiac defects and abnormalities of intestinal orientation in developing Xenopus laevis.
    Weeks DL
    Developmental biology 262.2 (2003 Oct 15): 268-81.

    Compensatory growth of healthy cardiac cells in the presence of diseased cells restores tissue homeostasis during heart development.
    Cox TC
    Developmental cell 15.4 (2008 Oct): 521-33.

    Requirement of a novel gene, Xin, in cardiac morphogenesis.
    Lin JJ
    Development (Cambridge, England) 126.6 (1999 Mar): 1281-94.

    Insulin-like growth factor (IGF) binding protein-5 blocks skeletal muscle differentiation by inhibiting IGF actions.
    Rotwein P
    Molecular endocrinology (Baltimore, Md.) 22.1 (2008 Jan): 206-15.

    Heart regeneration in adult Xenopus tropicalis after apical resection.
    Cai D
    Cell & bioscience 7. (2017): 70.

    WB References

    Forced expression and assembly of rat cardiac troponin T isoforms in cultured muscle and nonmuscle cells.
    Lin JJ
    Journal of muscle research and cell motility 14.6 (1993 Dec): 619-32.

    Muscle specific fragile X related protein 1 isoforms are sequestered in the nucleus of undifferentiated myoblast.
    Khandjian EW
    BMC genetics 1. (2000): 4.

    Alternative RNA splicing-generated cardiac troponin T isoform switching: a non-heart-restricted genetic programming synchronized in developing cardiac and skeletal muscles.
    Jin JP
    Biochemical and biophysical research communications 225.3 (1996 Aug 23): 883-9.

    Truncation by Glu180 nonsense mutation results in complete loss of slow skeletal muscle troponin T in a lethal nemaline myopathy.
    Crawford TO
    The Journal of biological chemistry 278.28 (2003 Jul 11): 26159-65.

    Conformational modulation of slow skeletal muscle troponin T by an NH(2)-terminal metal-binding extension.
    Huang QQ
    American journal of physiology. Cell physiology 279.4 (2000 Oct): C1067-77.

    Insulin-like growth factor (IGF) binding protein-5 blocks skeletal muscle differentiation by inhibiting IGF actions.
    Rotwein P
    Molecular endocrinology (Baltimore, Md.) 22.1 (2008 Jan): 206-15.

    Localization of the two tropomyosin-binding sites of troponin T.
    Chong SM
    Archives of biochemistry and biophysics 500.2 (2010 Aug 15): 144-50.

    IHC References

    Regulation of vertebrate embryogenesis by the exon junction complex core component Eif4a3.
    Weinstein DC
    Developmental dynamics : an official publication of the American Association of Anatomists 239.7 (2010 Jul): 1977-87.

    Truncation by Glu180 nonsense mutation results in complete loss of slow skeletal muscle troponin T in a lethal nemaline myopathy.
    Crawford TO
    The Journal of biological chemistry 278.28 (2003 Jul 11): 26159-65.

    CUG-BP, Elav-like family member 1 (CELF1) is required for normal myofibrillogenesis, morphogenesis, and contractile function in the embryonic heart.
    Ladd AN
    Developmental dynamics : an official publication of the American Association of Anatomists 245.8 (2016 Aug): 854-73.

    CUG-BP, Elav-like family member 1 (CELF1) is required for normal myofibrillogenesis, morphogenesis, and contractile function in the embryonic heart.
    Ladd AN
    Developmental dynamics : an official publication of the American Association of Anatomists 245.8 (2016 Aug): 854-73.

    FACS References

    Heterogeneity of Mesp1+ mesoderm revealed by single-cell RNA-seq.
    Kyba M
    Biochemical and biophysical research communications 474.3 (2016 Jun 3): 469-475.

    Epitope Map References

    Localization of the two tropomyosin-binding sites of troponin T.
    Chong SM
    Archives of biochemistry and biophysics 500.2 (2010 Aug 15): 144-50.

    All References

    Regulation of vertebrate embryogenesis by the exon junction complex core component Eif4a3.
    Weinstein DC
    Developmental dynamics : an official publication of the American Association of Anatomists 239.7 (2010 Jul): 1977-87.

    Truncation by Glu180 nonsense mutation results in complete loss of slow skeletal muscle troponin T in a lethal nemaline myopathy.
    Crawford TO
    The Journal of biological chemistry 278.28 (2003 Jul 11): 26159-65.

    CUG-BP, Elav-like family member 1 (CELF1) is required for normal myofibrillogenesis, morphogenesis, and contractile function in the embryonic heart.
    Ladd AN
    Developmental dynamics : an official publication of the American Association of Anatomists 245.8 (2016 Aug): 854-73.

    Precocious appearance of cardiac troponin T pre-mRNAs during early avian embryonic skeletal muscle development in ovo.
    Solursh M
    Developmental biology 140.1 (1990 Jul): 73-82.

    Forced expression and assembly of rat cardiac troponin T isoforms in cultured muscle and nonmuscle cells.
    Lin JJ
    Journal of muscle research and cell motility 14.6 (1993 Dec): 619-32.

    Confocal imaging of early heart development in Xenopus laevis.
    Weeks DL
    Developmental biology 218.1 (2000 Feb 1): 64-73.

    Pitx2c attenuation results in cardiac defects and abnormalities of intestinal orientation in developing Xenopus laevis.
    Weeks DL
    Developmental biology 262.2 (2003 Oct 15): 268-81.

    Compensatory growth of healthy cardiac cells in the presence of diseased cells restores tissue homeostasis during heart development.
    Cox TC
    Developmental cell 15.4 (2008 Oct): 521-33.

    Requirement of a novel gene, Xin, in cardiac morphogenesis.
    Lin JJ
    Development (Cambridge, England) 126.6 (1999 Mar): 1281-94.

    Insulin-like growth factor (IGF) binding protein-5 blocks skeletal muscle differentiation by inhibiting IGF actions.
    Rotwein P
    Molecular endocrinology (Baltimore, Md.) 22.1 (2008 Jan): 206-15.

    Heart regeneration in adult Xenopus tropicalis after apical resection.
    Cai D
    Cell & bioscience 7. (2017): 70.

    Muscle specific fragile X related protein 1 isoforms are sequestered in the nucleus of undifferentiated myoblast.
    Khandjian EW
    BMC genetics 1. (2000): 4.

    Alternative RNA splicing-generated cardiac troponin T isoform switching: a non-heart-restricted genetic programming synchronized in developing cardiac and skeletal muscles.
    Jin JP
    Biochemical and biophysical research communications 225.3 (1996 Aug 23): 883-9.

    Conformational modulation of slow skeletal muscle troponin T by an NH(2)-terminal metal-binding extension.
    Huang QQ
    American journal of physiology. Cell physiology 279.4 (2000 Oct): C1067-77.

    Localization of the two tropomyosin-binding sites of troponin T.
    Chong SM
    Archives of biochemistry and biophysics 500.2 (2010 Aug 15): 144-50.

    Heterogeneity of Mesp1+ mesoderm revealed by single-cell RNA-seq.
    Kyba M
    Biochemical and biophysical research communications 474.3 (2016 Jun 3): 469-475.

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