5D3-s

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SKU: 5D3-s

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DSHB Data Sheet
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Catalog Fields

Clone ID/Product Name: 5D3-s
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Symbol: Cdh1
Ab Isotype: MIgG2b
Gene Name:
Antibody Registry ID: AB_528116 
Uniprot ID: P30944 
RRID:  
Entrez Gene ID: 100337618 
Clonality: Monoclonal
Immunogen: Purified E-cadherin extracellular domain (100 kDa trypsin fragment ectodomain) from Xenopus A6 kidney cell line.
Clone:
Immunogen Sequence: partial protein
Myeloma Strain: sp-2/0
Epitope Mapped: Yes
Antigen Name: Cadherin, E-
Epitope Location or Sequence: Extracellular domain
Alternate Antigen Name:
Deposit Date: 5/7/1993
Antigen Molecular Weight:
Depositor: Gumbiner, B.
Antigen Sequence:
Depositor Institution: University of Virginia
Antigen Species: Xenopus
Depositor Notes: Blocks epithelial cell-cell adhesion and cell junction formation. Stains ectoderm, all epithelia and regions of cell-cell contact. E-cadherin-specific by immunoblotting and immunoprecipitation.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Drosophila, Xenopus
Additional Information: 5D3 binds both the - and + (Ca++) conformations of E-cadherin
Predicted Species Reactivity:  
Human Protein Atlas:  
Additional Characterization:  
Recommended Applications: FFPE, Function Blocking, Immunofluorescence, Immunoprecipitation, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
5D3-s was deposited to the DSHB by Gumbiner, B. (DSHB Hybridoma Product 5D3-s)
Storage and Handling Recommendations
Usage Recommendations

13 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • IP References
  • FFPE References
  • FB References
  • Epitope Map References
  • All References
    • Initial Publication

    Expression of cell adhesion molecule E-cadherin in Xenopus embryos begins at gastrulation and predominates in the ectoderm.
    Gumbiner B
    The Journal of cell biology 108.6 (1989 Jun): 2449-58.

    IF References

    Radial intercalation of ciliated cells during Xenopus skin development.
    Kintner C
    Development (Cambridge, England) 133.13 (2006 Jul): 2507-15.

    Specializations of intercellular junctions are associated with the presence and absence of hair cell regeneration in ears from six vertebrate classes.
    Corwin JT
    The Journal of comparative neurology 521.6 (2013 Apr 15): 1430-48.

    Specification of ion transport cells in the Xenopus larval skin.
    Kintner C
    Development (Cambridge, England) 138.4 (2011 Feb): 705-14.

    Expression of cell adhesion molecule E-cadherin in Xenopus embryos begins at gastrulation and predominates in the ectoderm.
    Gumbiner B
    The Journal of cell biology 108.6 (1989 Jun): 2449-58.

    The distribution of E-cadherin during Xenopus laevis development.
    Thiery JP
    Development (Cambridge, England) 111.1 (1991 Jan): 159-69.

    Temporal regulation of global gene expression and cellular morphology in Xenopus kidney cells in response to clinorotation.
    Asashima M
    Advances in space research : the official journal of the Committee on Space Research (COSPAR) 35.9 (2005): 1654-61.

    Chase-and-run between adjacent cell populations promotes directional collective migration.
    Mayor R
    Nature cell biology 15.7 (2013 Jul): 763-72.

    Cadherin Switch during EMT in Neural Crest Cells Leads to Contact Inhibition of Locomotion via Repolarization of Forces.
    Mayor R
    Developmental cell 34.4 (2015 Aug 24): 421-34.

    Visualization of adult stem cells within their niches using the Drosophila germline as a model system.
    Shcherbata HR
    Methods in molecular biology (Clifton, N.J.) 1035. (2013): 25-33.

    WB References

    Expression of cell adhesion molecule E-cadherin in Xenopus embryos begins at gastrulation and predominates in the ectoderm.
    Gumbiner B
    The Journal of cell biology 108.6 (1989 Jun): 2449-58.

    Foregut mesenchyme contributes cells to pancreatic acini during embryonic development in a chick-quail chimera model.
    Johnson PR
    Pediatric surgery international 21.3 (2005 Mar): 138-42.

    Selective disruption of E-cadherin function in early Xenopus embryos by a dominant negative mutant.
    Gumbiner BM
    Development (Cambridge, England) 120.4 (1994 Apr): 901-9.

    IHC References

    Tyrosine kinase inhibitors protect the salivary gland from radiation damage by increasing DNA double-strand break repair.
    Reyland ME
    The Journal of biological chemistry 296. (2021 Jan-Jun): 100401.

    IP References

    Expression of cell adhesion molecule E-cadherin in Xenopus embryos begins at gastrulation and predominates in the ectoderm.
    Gumbiner B
    The Journal of cell biology 108.6 (1989 Jun): 2449-58.

    Foregut mesenchyme contributes cells to pancreatic acini during embryonic development in a chick-quail chimera model.
    Johnson PR
    Pediatric surgery international 21.3 (2005 Mar): 138-42.

    FFPE References

    The cell sorting process of Xenopus gastrula cells involves the acto-myosin system and TGF-β signaling.
    Ihara S
    In vitro cellular & developmental biology. Animal 49.3 (2013 Mar): 220-9.

    FB References

    Expression of cell adhesion molecule E-cadherin in Xenopus embryos begins at gastrulation and predominates in the ectoderm.
    Gumbiner B
    The Journal of cell biology 108.6 (1989 Jun): 2449-58.

    Cadherin Switch during EMT in Neural Crest Cells Leads to Contact Inhibition of Locomotion via Repolarization of Forces.
    Mayor R
    Developmental cell 34.4 (2015 Aug 24): 421-34.

    Epitope Map References

    Expression of cell adhesion molecule E-cadherin in Xenopus embryos begins at gastrulation and predominates in the ectoderm.
    Gumbiner B
    The Journal of cell biology 108.6 (1989 Jun): 2449-58.

    All References

    Tyrosine kinase inhibitors protect the salivary gland from radiation damage by increasing DNA double-strand break repair.
    Reyland ME
    The Journal of biological chemistry 296. (2021 Jan-Jun): 100401.

    Radial intercalation of ciliated cells during Xenopus skin development.
    Kintner C
    Development (Cambridge, England) 133.13 (2006 Jul): 2507-15.

    Specializations of intercellular junctions are associated with the presence and absence of hair cell regeneration in ears from six vertebrate classes.
    Corwin JT
    The Journal of comparative neurology 521.6 (2013 Apr 15): 1430-48.

    Specification of ion transport cells in the Xenopus larval skin.
    Kintner C
    Development (Cambridge, England) 138.4 (2011 Feb): 705-14.

    Expression of cell adhesion molecule E-cadherin in Xenopus embryos begins at gastrulation and predominates in the ectoderm.
    Gumbiner B
    The Journal of cell biology 108.6 (1989 Jun): 2449-58.

    The distribution of E-cadherin during Xenopus laevis development.
    Thiery JP
    Development (Cambridge, England) 111.1 (1991 Jan): 159-69.

    Temporal regulation of global gene expression and cellular morphology in Xenopus kidney cells in response to clinorotation.
    Asashima M
    Advances in space research : the official journal of the Committee on Space Research (COSPAR) 35.9 (2005): 1654-61.

    Chase-and-run between adjacent cell populations promotes directional collective migration.
    Mayor R
    Nature cell biology 15.7 (2013 Jul): 763-72.

    Cadherin Switch during EMT in Neural Crest Cells Leads to Contact Inhibition of Locomotion via Repolarization of Forces.
    Mayor R
    Developmental cell 34.4 (2015 Aug 24): 421-34.

    Visualization of adult stem cells within their niches using the Drosophila germline as a model system.
    Shcherbata HR
    Methods in molecular biology (Clifton, N.J.) 1035. (2013): 25-33.

    Foregut mesenchyme contributes cells to pancreatic acini during embryonic development in a chick-quail chimera model.
    Johnson PR
    Pediatric surgery international 21.3 (2005 Mar): 138-42.

    Selective disruption of E-cadherin function in early Xenopus embryos by a dominant negative mutant.
    Gumbiner BM
    Development (Cambridge, England) 120.4 (1994 Apr): 901-9.

    The cell sorting process of Xenopus gastrula cells involves the acto-myosin system and TGF-β signaling.
    Ihara S
    In vitro cellular & developmental biology. Animal 49.3 (2013 Mar): 220-9.

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