sm 1.2

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SKU: sm 1.2-s
View product citations for antibody sm 1.2 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: somatomedin-C; Sm-C/IGF-1
Hybridoma Cells Available: No
Antigen Species: human
Depositor: Van Wyk, J.J. / Underwood, L.E.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: University of North Carolina Chapel Hill
Positive Tested Species Reactivity: Chick, Human, Mouse, Rat
Depositors Notes: Species specificity note: cross-reacts with human, rat, mouse, and possibly chick; occasional difficulty neutralizing rat somatomedin due to binding protein interference. Cell binding note: demonstrated in Sertoli cells, testicular peritubular cells, brain cells, liver cells and many other cell types. Antibody competition: cross-reacts with IGF-II but not other peptide hormones. Blotting note: single band in Western blot with polyclonal Ab. Purification note: single band on PAGE with silver stain. Functional effects: promotes cell replication and differentiation in wide variety of in vitro systems. IHC note: in situ hybridization histochemistry reveals origin in mesenchymally derived tissues (Han et al., Science (1987). 236, 193-196.), but immunofluorescence shows more diffuse localization (Han et al., Pediatr. Res. (1987).
Antigen Molecular Weight: 7.5 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: P3X63Ag8.653
Epitope Location or Sequence:
Uniprot ID:  
Immunogen Sequence:
Entrez Gene ID:  
Additional Characterization:  
Antibody Registry ID: AB_2233416 
Additional Information:
Recommended Applications: Immunofluorescence, Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
sm 1.2 was deposited to the DSHB by Van Wyk, J.J. / Underwood, L.E. (DSHB Hybridoma Product sm 1.2)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
All cell products contain the antimicrobial ProClin. Click here for additional information.

3 References

  • Initial Publication
  • IF References
  • IHC References
  • All References
  • Initial Publication
    IF References
    IHC References
    All References

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