Antibody Supernatant (-s)

    • 10% IMDM.
    • 88% ADCF MAb (a serum-free medium).   The DSHB has switched from and older serum free medium called HB101.  Older lots of supernatant (prior to 2015) may contain HB101 instead of ADCF MAb.
    • 2.0% Fetal Bovine Serum.
    • 50ug/ml  Gentamicin
    • 30mM Tris-HCL
    • 0.0015% ProClin 150

    Antibody Concentrate (-c)

    • 1X DPBS (20 mM phosphate buffer containing 150mM NaCl)
    • 0.0015% ProClin 150
    • Antibody concentrate storage buffer contains other proteins greater than 50kDa (the size cutoff for the filter used to make the concentrate) that were present in the supernatant from which the concentrate was made.  This includes BSA from the FBS in the growth media as well as other proteins resulting from cell death and lysis.

    Antibody Bioreactor Supernatant (-b)

    • IMDM.
    • 20% Fetal Bovine Serum ultra-low IgG
    • 50ug/ml Gentamicin
    • 30mM Tris-HCL
    • 0.0015% ProClin 150

    Frozen Hybridoma Cells (-f)

    • ADCF MAb (a serum-free medium).
    • 50ug/ml Gentamicin
    • 6% DMSO

    Growing Hybridoma Cells (-g)

    • IMDM.
    • 20% Fetal Bovine Serum
    • 50ug/ml Gentamicin

    BSA concentration in DSHB antibody products

    • Supernatant:  <0.03%
    • Concentrate:  <0.3%
    • Bioreactor Supe:  <0.3%

    ProClin 150 concentration in DSHB antibody products

    • 15ppm or 0.0015%

    FBS concentration in all DSHB products

    • Supernatant:  2.0%
    • Concentrate:  <0.1%
    • Bioreactor Supe:  20%
    • Frozen cells: serum-free.
    • Growing cells: 20%