tor23

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SKU: tor23
View product citations for antibody tor23 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: Acetylcholinesterase, presynaptic
Hybridoma Cells Available: Yes
Antigen Species: Torpedo
Depositor: Harland, Richard
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: University of California, Berkeley
Positive Tested Species Reactivity: Human, Rat, Torpedo, Xenopus
Depositors Notes: This antibody stains spinal cord, cerebral cortex and pyramidal cells in the hippocampal CA2 region. The antigen recognized by Tor23 is also expressed in hNTERA-2 teratocarcinoma.
Antigen Molecular Weight: 180 kDa, 66.7 kDa
Human Protein Atlas:
Predicted Species Reactivity:  
Gene: ache
Immunogen: Purified synaptosomes from Torpedo electric organ
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped:
Myeloma Strain: NS1
Epitope Location or Sequence:
Uniprot ID: P07692 
Immunogen Sequence:
Entrez Gene ID:
Additional Characterization:
Antibody Registry ID: AB_2722484 
Additional Information: This antibody was generated by Pinky Kushner in 1984 as part of a library of monoclonal antibodies to Torpedo cholinergic synaptosomes. RRID:AB_2722484
Recommended Applications: ELISA, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
tor23 was deposited to the DSHB by Harland, Richard (DSHB Hybridoma Product tor23)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

8 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • IP References
  • ELISA References
  • All References
  • Initial Publication

    A library of monoclonal antibodies to Torpedo cholinergic synaptosomes.
    Kushner PD
    Journal of neurochemistry 43.3 (1984 Sep): 775-86.

    Monoclonal antibody Tor 23 recognizes a determinant of a presynaptic acetylcholinesterase.
    Weber R
    Journal of neurochemistry 48.6 (1987 Jun): 1942-53.

    IF References

    Torpedo monoclonal antibodies react with components of the human peripheral nervous system.
    Kushner PD
    Muscle and nerve 11.1 (1988 Jan): 10-20.

    Monoclonal antibody Tor 23 recognizes a determinant of a presynaptic acetylcholinesterase.
    Weber R
    Journal of neurochemistry 48.6 (1987 Jun): 1942-53.

    A human teratocarcinoma which expresses a rare neuronal cell surface antigen.
    Woloshin PI
    Brain research 388.3 (1987 Sep): 271-5.

    An atlas of a rare neuronal surface antigen in the rat central nervous system.
    Kushner PD
    The Journal of neuroscience : the official journal of the Society for Neuroscience 8.8 (1988 Aug): 3035-56.

    Monoclonal antibody Tor 23 binds a subset of neural cells in the human cortex and displays an altered binding distribution in Alzheimer's disease.
    Greco CM
    Journal of neuropathology and experimental neurology 48.6 (1989 Nov): 692-708.

    Family of human neuronal external surface epitopes defined by Torpedo monoclonal antibodies.
    Kushner PD
    Journal of neuroscience research 25.4 (1990 Apr): 486-502.

    WB References

    A synaptic vesicle antigen is restricted to the junctional region of the presynaptic plasma membrane.
    Kelly RB
    Proceedings of the National Academy of Sciences of the United States of America 80.23 (1983 Dec): 7342-6.

    Monoclonal antibody Tor 23 recognizes a determinant of a presynaptic acetylcholinesterase.
    Weber R
    Journal of neurochemistry 48.6 (1987 Jun): 1942-53.

    Monoclonal antibody Tor 23 binds a subset of neural cells in the human cortex and displays an altered binding distribution in Alzheimer's disease.
    Greco CM
    Journal of neuropathology and experimental neurology 48.6 (1989 Nov): 692-708.

    Family of human neuronal external surface epitopes defined by Torpedo monoclonal antibodies.
    Kushner PD
    Journal of neuroscience research 25.4 (1990 Apr): 486-502.

    IHC References

    Family of human neuronal external surface epitopes defined by Torpedo monoclonal antibodies.
    Kushner PD
    Journal of neuroscience research 25.4 (1990 Apr): 486-502.

    Monoclonal antibody Tor 23 recognizes a determinant of a presynaptic acetylcholinesterase.
    Weber R
    Journal of neurochemistry 48.6 (1987 Jun): 1942-53.

    A synaptic vesicle antigen is restricted to the junctional region of the presynaptic plasma membrane.
    Kelly RB
    Proceedings of the National Academy of Sciences of the United States of America 80.23 (1983 Dec): 7342-6.

    An atlas of a rare neuronal surface antigen in the rat central nervous system.
    Kushner PD
    The Journal of neuroscience : the official journal of the Society for Neuroscience 8.8 (1988 Aug): 3035-56.

    IP References

    A synaptic vesicle antigen is restricted to the junctional region of the presynaptic plasma membrane.
    Kelly RB
    Proceedings of the National Academy of Sciences of the United States of America 80.23 (1983 Dec): 7342-6.

    Monoclonal antibody Tor 23 recognizes a determinant of a presynaptic acetylcholinesterase.
    Weber R
    Journal of neurochemistry 48.6 (1987 Jun): 1942-53.

    ELISA References
    All References

    Family of human neuronal external surface epitopes defined by Torpedo monoclonal antibodies.
    Kushner PD
    Journal of neuroscience research 25.4 (1990 Apr): 486-502.

    Monoclonal antibody Tor 23 recognizes a determinant of a presynaptic acetylcholinesterase.
    Weber R
    Journal of neurochemistry 48.6 (1987 Jun): 1942-53.

    A synaptic vesicle antigen is restricted to the junctional region of the presynaptic plasma membrane.
    Kelly RB
    Proceedings of the National Academy of Sciences of the United States of America 80.23 (1983 Dec): 7342-6.

    An atlas of a rare neuronal surface antigen in the rat central nervous system.
    Kushner PD
    The Journal of neuroscience : the official journal of the Society for Neuroscience 8.8 (1988 Aug): 3035-56.

    Torpedo monoclonal antibodies react with components of the human peripheral nervous system.
    Kushner PD
    Muscle and nerve 11.1 (1988 Jan): 10-20.

    A human teratocarcinoma which expresses a rare neuronal cell surface antigen.
    Woloshin PI
    Brain research 388.3 (1987 Sep): 271-5.

    Monoclonal antibody Tor 23 binds a subset of neural cells in the human cortex and displays an altered binding distribution in Alzheimer's disease.
    Greco CM
    Journal of neuropathology and experimental neurology 48.6 (1989 Nov): 692-708.

    A library of monoclonal antibodies to Torpedo cholinergic synaptosomes.
    Kushner PD
    Journal of neurochemistry 43.3 (1984 Sep): 775-86.

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