tor70/Notochord Ab1

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$40.00
SKU: tor70/Notochord Ab1
View product citations for antibody tor70/Notochord Ab1 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: Synaptic vesicle proteoglycan/Notochord marker
Hybridoma Cells Available: Yes
Antigen Species: Torpedo
Depositor: Harland, Richard
Isotype: MIgM
Antigen Sequence:
Host Species: mouse
Depositors Institution: University of California, Berkeley
Positive Tested Species Reactivity: Axolotl, Human, Rana Pipiens, Rat, Torpedo, Xenopus, Zebrafish
Depositors Notes: This antibody was generated by Pinky Kushner in 1984. This antigen is also known as SV1 and labels a proteoglycan on the luminal face of synaptic vesicles.
Antigen Molecular Weight: 100-200 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene:
Immunogen: Purified cholinergic synaptosomes from Torpedo electric organ
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: NS1
Epitope Location or Sequence:
Uniprot ID:  
Immunogen Sequence:
Antibody Registry ID: AB_2722485 
Additional Information: This antibody was generated by Pinky Kushner in 1984 as part of a library of monoclonal antibodies to Torpedo cholinergic synaptosomes. RRID:AB_2722485
Recommended Applications: FFPE, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
tor70/Notochord Ab1 was deposited to the DSHB by Harland, Richard (DSHB Hybridoma Product tor70/Notochord Ab1)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

25 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • IP References
  • FFPE References
  • All References
  • Initial Publication

    A library of monoclonal antibodies to Torpedo cholinergic synaptosomes.
    Kushner PD
    Journal of neurochemistry 43.3 (1984 Sep): 775-86.

    Ventral ectoderm of Xenopus forms neural tissue, including hindbrain, in response to activin.
    Harland RM
    Development (Cambridge, England) 115.3 (1992 Jul): 681-8.

    IF References

    Torpedo monoclonal antibodies react with components of the human peripheral nervous system.
    Kushner PD
    Muscle & nerve 11.1 (1988 Jan): 10-20.

    Temporal and spatial patterning of axial myotome fibers in Xenopus laevis.
    Domingo CR
    Developmental dynamics : an official publication of the American Association of Anatomists 239.4 (2010 Apr): 1162-77.

    Embryonic cells depleted of beta-catenin remain competent to differentiate into dorsal mesodermal derivatives.
    Domingo CR
    Developmental dynamics : an official publication of the American Association of Anatomists 236.11 (2007 Nov): 3007-19.

    An adhesome comprising laminin, dystroglycan and myosin IIA is required during notochord development in Xenopus laevis.
    Bello V
    Development (Cambridge, England) 141.23 (2014 Dec): 4569-79.

    Temporal and spatial patterning of axial myotome fibers in Xenopus laevis.
    Domingo CR
    Developmental dynamics : an official publication of the American Association of Anatomists 239.4 (2010 Apr): 1162-77.

    Patterning and tissue movements in a novel explant preparation of the marginal zone of Xenopus laevis.
    DeSimone D
    Gene expression patterns : GEP 4.4 (2004 Jul): 457-66.

    Integrin-ECM interactions regulate cadherin-dependent cell adhesion and are required for convergent extension in Xenopus.
    DeSimone DW
    Current biology : CB 13.14 (2003 Jul 15): 1182-91.

    WB References

    A synaptic vesicle antigen is restricted to the junctional region of the presynaptic plasma membrane.
    Kelly RB
    Proceedings of the National Academy of Sciences of the United States of America 80.23 (1983 Dec): 7342-6.

    A highly antigenic proteoglycan-like component of cholinergic synaptic vesicles.
    Kelly RB
    The Journal of biological chemistry 258.18 (1983 Sep 25): 11082-91.

    IHC References

    Notch signaling modulates the nuclear localization of carboxy-terminal-phosphorylated smad2 and controls the competence of ectodermal cells for activin A.
    Asashima M
    Mechanisms of development 122.5 (2005 May): 671-80.

    Muscle specification in the Xenopus laevis gastrula-stage embryo.
    Domingo CR
    Developmental dynamics : an official publication of the American Association of Anatomists 233.4 (2005 Aug): 1348-58.

    Characterizing the zebrafish organizer: microsurgical analysis at the early-shield stage.
    Fraser SE
    Development (Cambridge, England) 122.4 (1996 Apr): 1313-22.

    Novel gene ashwin functions in Xenopus cell survival and anteroposterior patterning.
    Sater AK
    Developmental dynamics : an official publication of the American Association of Anatomists 235.7 (2006 Jul): 1895-907.

    Dapper, a Dishevelled-associated antagonist of beta-catenin and JNK signaling, is required for notochord formation.
    Moon RT
    Developmental cell 2.4 (2002 Apr): 449-61.

    A novel mesoderm inducer, Madr2, functions in the activin signal transduction pathway.
    Harland RM
    Genes & development 10.15 (1996 Aug 1): 1880-9.

    Embryonic expression and functional analysis of a Xenopus activin receptor.
    Melton DA
    Developmental dynamics : an official publication of the American Association of Anatomists 194.1 (1992 May): 1-11.

    A revised model of Xenopus dorsal midline development: differential and separable requirements for Notch and Shh signaling.
    Harland RM
    Developmental biology 352.2 (2011 Apr 15): 254-66.

    Vertical versus planar neural induction in Rana pipiens embryos.
    Dawid IB
    Proceedings of the National Academy of Sciences of the United States of America 91.8 (1994 Apr 12): 3049-53.

    Expression of the LIM class homeobox gene Xlim-1 in pronephros and CNS cell lineages of Xenopus embryos is affected by retinoic acid and exogastrulation.
    Dawid IB
    Development (Cambridge, England) 120.6 (1994 Jun): 1525-36.

    Microtubule disruption reveals that Spemann's organizer is subdivided into two domains by the vegetal alignment zone.
    Keller R
    Development (Cambridge, England) 124.4 (1997 Feb): 895-906.

    Xenopus fibrillin regulates directed convergence and extension.
    Keller R
    Developmental biology 301.2 (2007 Jan 15): 404-16.

    Serotonin signaling is a very early step in patterning of the left-right axis in chick and frog embryos.
    Levin M
    Current biology : CB 15.9 (2005 May 10): 794-803.

    Zygotic VegT is required for Xenopus paraxial mesoderm formation and is regulated by Nodal signaling and Eomesodermin.
    Asashima M
    The International journal of developmental biology 54.1 (2010): 81-92.

    Ventral ectoderm of Xenopus forms neural tissue, including hindbrain, in response to activin.
    Harland RM
    Development (Cambridge, England) 115.3 (1992 Jul): 681-8.

    The midline (notochord and notoplate) patterns the cell motility underlying convergence and extension of the Xenopus neural plate.
    Keller R
    Developmental biology 256.1 (2003 Apr 1): 100-14.

    IP References

    A synaptic vesicle antigen is restricted to the junctional region of the presynaptic plasma membrane.
    Kelly RB
    Proceedings of the National Academy of Sciences of the United States of America 80.23 (1983 Dec): 7342-6.

    A highly antigenic proteoglycan-like component of cholinergic synaptic vesicles.
    Kelly RB
    The Journal of biological chemistry 258.18 (1983 Sep 25): 11082-91.

    FFPE References
    All References

    Notch signaling modulates the nuclear localization of carboxy-terminal-phosphorylated smad2 and controls the competence of ectodermal cells for activin A.
    Asashima M
    Mechanisms of development 122.5 (2005 May): 671-80.

    Muscle specification in the Xenopus laevis gastrula-stage embryo.
    Domingo CR
    Developmental dynamics : an official publication of the American Association of Anatomists 233.4 (2005 Aug): 1348-58.

    Characterizing the zebrafish organizer: microsurgical analysis at the early-shield stage.
    Fraser SE
    Development (Cambridge, England) 122.4 (1996 Apr): 1313-22.

    Novel gene ashwin functions in Xenopus cell survival and anteroposterior patterning.
    Sater AK
    Developmental dynamics : an official publication of the American Association of Anatomists 235.7 (2006 Jul): 1895-907.

    Dapper, a Dishevelled-associated antagonist of beta-catenin and JNK signaling, is required for notochord formation.
    Moon RT
    Developmental cell 2.4 (2002 Apr): 449-61.

    A novel mesoderm inducer, Madr2, functions in the activin signal transduction pathway.
    Harland RM
    Genes & development 10.15 (1996 Aug 1): 1880-9.

    Embryonic expression and functional analysis of a Xenopus activin receptor.
    Melton DA
    Developmental dynamics : an official publication of the American Association of Anatomists 194.1 (1992 May): 1-11.

    A revised model of Xenopus dorsal midline development: differential and separable requirements for Notch and Shh signaling.
    Harland RM
    Developmental biology 352.2 (2011 Apr 15): 254-66.

    Vertical versus planar neural induction in Rana pipiens embryos.
    Dawid IB
    Proceedings of the National Academy of Sciences of the United States of America 91.8 (1994 Apr 12): 3049-53.

    Expression of the LIM class homeobox gene Xlim-1 in pronephros and CNS cell lineages of Xenopus embryos is affected by retinoic acid and exogastrulation.
    Dawid IB
    Development (Cambridge, England) 120.6 (1994 Jun): 1525-36.

    Microtubule disruption reveals that Spemann's organizer is subdivided into two domains by the vegetal alignment zone.
    Keller R
    Development (Cambridge, England) 124.4 (1997 Feb): 895-906.

    Xenopus fibrillin regulates directed convergence and extension.
    Keller R
    Developmental biology 301.2 (2007 Jan 15): 404-16.

    Serotonin signaling is a very early step in patterning of the left-right axis in chick and frog embryos.
    Levin M
    Current biology : CB 15.9 (2005 May 10): 794-803.

    Zygotic VegT is required for Xenopus paraxial mesoderm formation and is regulated by Nodal signaling and Eomesodermin.
    Asashima M
    The International journal of developmental biology 54.1 (2010): 81-92.

    Ventral ectoderm of Xenopus forms neural tissue, including hindbrain, in response to activin.
    Harland RM
    Development (Cambridge, England) 115.3 (1992 Jul): 681-8.

    The midline (notochord and notoplate) patterns the cell motility underlying convergence and extension of the Xenopus neural plate.
    Keller R
    Developmental biology 256.1 (2003 Apr 1): 100-14.

    Torpedo monoclonal antibodies react with components of the human peripheral nervous system.
    Kushner PD
    Muscle & nerve 11.1 (1988 Jan): 10-20.

    Temporal and spatial patterning of axial myotome fibers in Xenopus laevis.
    Domingo CR
    Developmental dynamics : an official publication of the American Association of Anatomists 239.4 (2010 Apr): 1162-77.

    Embryonic cells depleted of beta-catenin remain competent to differentiate into dorsal mesodermal derivatives.
    Domingo CR
    Developmental dynamics : an official publication of the American Association of Anatomists 236.11 (2007 Nov): 3007-19.

    An adhesome comprising laminin, dystroglycan and myosin IIA is required during notochord development in Xenopus laevis.
    Bello V
    Development (Cambridge, England) 141.23 (2014 Dec): 4569-79.

    Patterning and tissue movements in a novel explant preparation of the marginal zone of Xenopus laevis.
    DeSimone D
    Gene expression patterns : GEP 4.4 (2004 Jul): 457-66.

    Integrin-ECM interactions regulate cadherin-dependent cell adhesion and are required for convergent extension in Xenopus.
    DeSimone DW
    Current biology : CB 13.14 (2003 Jul 15): 1182-91.

    A synaptic vesicle antigen is restricted to the junctional region of the presynaptic plasma membrane.
    Kelly RB
    Proceedings of the National Academy of Sciences of the United States of America 80.23 (1983 Dec): 7342-6.

    A highly antigenic proteoglycan-like component of cholinergic synaptic vesicles.
    Kelly RB
    The Journal of biological chemistry 258.18 (1983 Sep 25): 11082-91.

    A library of monoclonal antibodies to Torpedo cholinergic synaptosomes.
    Kushner PD
    Journal of neurochemistry 43.3 (1984 Sep): 775-86.

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