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13G3B7 Fibronectin III-15

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$45.00 to $425.00
SKU: 13G3B7 Fibronectin III-15
View product citations for antibody 13G3B7 Fibronectin III-15 on CiteAb

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Available: 34

DSHB Data Sheet

Catalog Fields

Antigen: fibronectin, final type III repeat
Available to For-Profits: Yes
Antigen Species: human
Hybridoma Cells Available (Non-Profit): Yes
Isotype: MIgG1
Depositor: Burton-Wurster, N.
Host Species: mouse
Antigen Sequence:
Positive Tested Species Reactivity: Canine, Human
Depositors Institution: Cornell University, Baker Institute for Animal Health, College of Veterinary Medicine
Antigen Molecular Weight: Predicted: multiple isoforms between 218 and 256 kDa and one or two isoforms at 69-71 kDa, plus shorter cleavage products.
Depositors Notes: May work on rat and horse
Predicted Species Reactivity:  
Human Protein Atlas:  
Immunogen: Purified fibronectin from human synovial fluid
Gene: FN1
Alternate Antibody Name: 13G3B7
Alternate Gene Names: FN, CIG, FNZ, MSF, ED-B, FINC, GFND, LETS, GFND2
Alternate Antigen Name:
Clonality: Monoclonal
Myeloma Strain: Sp2/0
Epitope Mapped: Yes
Uniprot ID: P02751 
Epitope Location or Sequence: Final type III repeat of canine fibronectin
Entrez Gene ID: 2335 
Immunogen Sequence: Full length protein
Antibody Registry ID: AB_2105842 
Additional Characterization:  
Recommended Applications: ELISA, Immunofluorescence, Western Blot
Additional Information:
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
13G3B7 Fibronectin III-15 was deposited to the DSHB by Burton-Wurster, N. (DSHB Hybridoma Product 13G3B7 Fibronectin III-15)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

2 References

  • Initial Publication
  • WB References
  • ELISA References
  • Epitope Map References
  • All References
  • Initial Publication

    Molecular and immunologic differences in canine fibronectins from articular cartilage and plasma.
    Lust G
    Archives of biochemistry and biophysics 269.1 (1989 Feb 15): 32-45.

    WB References

    Molecular and immunologic differences in canine fibronectins from articular cartilage and plasma.
    Lust G
    Archives of biochemistry and biophysics 269.1 (1989 Feb 15): 32-45.

    The cartilage-specific (V+C)- fibronectin isoform exists primarily in homodimeric and monomeric configurations.
    MacLeod JN
    The Biochemical journal 341 ( Pt 3).Pt 3 (1999 Aug 1): 555-61.

    ELISA References

    Molecular and immunologic differences in canine fibronectins from articular cartilage and plasma.
    Lust G
    Archives of biochemistry and biophysics 269.1 (1989 Feb 15): 32-45.

    Epitope Map References

    Molecular and immunologic differences in canine fibronectins from articular cartilage and plasma.
    Lust G
    Archives of biochemistry and biophysics 269.1 (1989 Feb 15): 32-45.

    All References

    Molecular and immunologic differences in canine fibronectins from articular cartilage and plasma.
    Lust G
    Archives of biochemistry and biophysics 269.1 (1989 Feb 15): 32-45.

    The cartilage-specific (V+C)- fibronectin isoform exists primarily in homodimeric and monomeric configurations.
    MacLeod JN
    The Biochemical journal 341 ( Pt 3).Pt 3 (1999 Aug 1): 555-61.

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