13G3B7 Fibronectin III-15
Catalog Fields
Antigen: fibronectin, final type III repeat
Hybridoma Cells Available: Yes
Antigen Species: human
Depositor: Burton-Wurster, N.
Isotype: MIgG1
Depositors Institution: Baker Institute for Animal Health, College of Veterinary Medicine, Cornell University
Host Species: mouse
Depositors Notes: May work on rat and horse
Positive Tested Species Reactivity: Canine, Human
Gene: FN1
Antigen Molecular Weight: Predicted: multiple isoforms between 218 and 256 kDa and one or two isoforms at 69-71 kDa, plus shorter cleavage products.
Alternate Gene Names: FN, CIG, FNZ, MSF, ED-B, FINC, GFND, LETS, GFND2
Immunogen: Purified fibronectin from human synovial fluid
Clonality: Monoclonal
Alternate Antibody Name: 13G3B7
Epitope Mapped: Yes
Myeloma Strain: Sp2/0
Epitope Location or Sequence: Final type III repeat of canine fibronectin
Uniprot ID: P02751
Immunogen Sequence: Full length protein
Entrez Gene ID: 2335
Antibody Registry ID: AB_2105842
Recommended Applications: ELISA, Immunofluorescence, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
13G3B7 Fibronectin III-15 was deposited to the DSHB by Burton-Wurster, N. (DSHB Hybridoma Product 13G3B7 Fibronectin III-15)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.
2 References
Initial Publication
Molecular and immunologic differences in canine fibronectins from articular cartilage and plasma.
Lust G
Archives of biochemistry and biophysics 269.1 (1989 Feb 15): 32-45.