HFN 7.1

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$40.00
SKU: HFN 7.1
View product citations for antibody HFN 7.1 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: fibronectin, human, type III repeats 9 and 10, function blocking
Hybridoma Cells Available: Yes
Antigen Species: Human
Depositor: Klebe, R.J.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: The University of Texas Health Science Center at San Antonio
Positive Tested Species Reactivity: Human
Depositors Notes: Specific to human fibronectin. HFN 7.1 interferes with the attachment of fibronectin to its receptor on the cell surface and inhibits fibronectin mediated cell adhesion.
Antigen Molecular Weight: Predicted: multiple isoforms between 218 and 256 kDa and one or two isoforms at 69-71 kDa, plus shorter cleavage products.
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: FN1
Immunogen: Human fibronectin purified from serum
Alternate Gene Names: FN, CIG, FNZ, MSF, ED-B, FINC, GFND, LETS, GFND2
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: P3-X63-Ag8
Epitope Location or Sequence: Between the PHSRN synergy and RGD sites (spans the connection between type III repeats 9 and 10)
Uniprot ID: P02751 
Immunogen Sequence: Full length protein
Entrez Gene ID: 2335 
Additional Characterization:  
Antibody Registry ID: AB_528244 
Additional Information:
Recommended Applications: ELISA, Function Blocking, Immunofluorescence, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
HFN 7.1 was deposited to the DSHB by Klebe, R.J. (DSHB Hybridoma Product HFN 7.1)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

13 References

  • Initial Publication
  • IF References
  • WB References
  • ELISA References
  • FB References
  • Epitope Map References
  • All References
  • Initial Publication
    IF References
    WB References
    ELISA References

    Monoclonal antibody against human fibronectin which inhibits cell attachment.
    Klebe RJ
    Hybridoma 1.2 (1982): 99-108.

    Assignment of a fibronection gene to human chromosome 2 using monoclonal antibodies.
    Shows TB
    Experimental cell research 141.2 (1982 Oct): 293-302.

    Surface chemistry gradients on silicone elastomers for high-throughput modulation of cell-adhesive interfaces.
    Gallant ND
    Journal of biomedical materials research. Part A 103.6 (2015 Jun): 2066-76.

    FB References
    Epitope Map References

    Integrin alpha IIb beta 3 (platelet GPIIb-IIIa) recognizes multiple sites in fibronectin.
    Ginsberg MH
    The Journal of biological chemistry 266.34 (1991 Dec 5): 23323-8.

    All References

    Monoclonal antibody against human fibronectin which inhibits cell attachment.
    Klebe RJ
    Hybridoma 1.2 (1982): 99-108.

    Assignment of a fibronection gene to human chromosome 2 using monoclonal antibodies.
    Shows TB
    Experimental cell research 141.2 (1982 Oct): 293-302.

    Expression patterns of extracellular matrix components in native and cultured normal human thyroid tissue and in human toxic adenoma tissue.
    Bürgi U
    Thyroid : official journal of the American Thyroid Association 7.3 (1997 Jun): 347-56.

    Stretching-induced nanostructures on shape memory polyurethane films and their regulation to osteoblasts morphology.
    Luo Y
    Colloids and surfaces. B, Biointerfaces 146. (2016 Oct 1): 431-41.

    Characterisation of human gingival neural crest-derived stem cells in monolayer and neurosphere cultures.
    Häkkinen L
    European cells & materials 31. (2016 Jan 5): 40-58.

    Fibronectin enhances Campylobacter fetus interaction with extracellular matrix components and INT 407 cells.
    Woodman RL
    Canadian journal of microbiology 54.1 (2008 Jan): 37-47.

    Mammary epithelial cell interactions with fibronectin stimulate epithelial-mesenchymal transition.
    Schwarzbauer JE
    Oncogene 33.13 (2014 Mar 27): 1649-57.

    Proteomic network analysis of human uterine smooth muscle in pregnancy, labor, and preterm labor.
    Buxton IL
    Integrative molecular medicine 2.4 (2015 Aug): 261-269.

    Increased Expression of Beige/Brown Adipose Markers from Host and Breast Cancer Cells Influence Xenograft Formation in Mice.
    Pervin S
    Molecular cancer research : MCR 14.1 (2016 Jan): 78-92.

    Laminin receptor 37/67LR regulates adhesion and proliferation of normal human intestinal epithelial cells.
    Beaulieu JF
    PloS one 8.8 (2013): e74337.

    Surface chemistry gradients on silicone elastomers for high-throughput modulation of cell-adhesive interfaces.
    Gallant ND
    Journal of biomedical materials research. Part A 103.6 (2015 Jun): 2066-76.

    Integrin alpha IIb beta 3 (platelet GPIIb-IIIa) recognizes multiple sites in fibronectin.
    Ginsberg MH
    The Journal of biological chemistry 266.34 (1991 Dec 5): 23323-8.

    Microinjection of antifibronectin antibodies in the chicken blastoderm: inhibition of mesoblast cell migration but not of cell ingression at the primitive streak.
    Foidart JM
    The Anatomical record 236.4 (1993 Aug): 685-96.

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