HFN 7.1
Catalog Fields
Antigen: fibronectin, human, type III repeats 9 and 10, function blocking
Available to For-Profits: Yes
Antigen Species: Human
Hybridoma Cells Available (Non-Profit): Yes
Isotype: MIgG1
Depositor: Klebe, R.J.
Host Species: mouse
Antigen Sequence:
Positive Tested Species Reactivity: Human
Depositors Institution: University of Texas Health Science Center at San Antonio
Antigen Molecular Weight: Predicted: multiple isoforms between 218 and 256 kDa and one or two isoforms at 69-71 kDa, plus shorter cleavage products.
Depositors Notes: Specific to human fibronectin. HFN 7.1 interferes with the attachment of fibronectin to its receptor on the cell surface and inhibits fibronectin mediated cell adhesion.
Immunogen: Human fibronectin purified from serum
Gene: FN1
Alternate Antibody Name:
Alternate Gene Names: FN, CIG, FNZ, MSF, ED-B, FINC, GFND, LETS, GFND2
Alternate Antigen Name:
Clonality: Monoclonal
Myeloma Strain: P3-X63-Ag8
Epitope Mapped: Yes
Uniprot ID: P02751
Epitope Location or Sequence: Between the PHSRN synergy and RGD sites (spans the connection between type III repeats 9 and 10)
Entrez Gene ID: 2335
Immunogen Sequence: Full length protein
Antibody Registry ID: AB_528244
Recommended Applications: ELISA, Function Blocking, Immunofluorescence, Western Blot
Additional Information:
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
HFN 7.1 was deposited to the DSHB by Klebe, R.J. (DSHB Hybridoma Product HFN 7.1)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
13 References
Initial Publication
Monoclonal antibody against human fibronectin which inhibits cell attachment.
Klebe RJ
Hybridoma 1.2 (1982): 99-108.