1D1

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$40.00
SKU: 1D1
View product citations for antibody 1D1 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: neurocan (C-terminal epitope)
Hybridoma Cells Available: Yes
Antigen Species: rat
Depositor: Margolis, R.U. / Margolis, R.K.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: Department of Pharmacology Department of Pharmacology New York University Medical Center
Positive Tested Species Reactivity: Rat
Depositors Notes: For chemical properties of the antigen see Rauch et al, 1991, 1992; Margolis et al., 1996; Margolis and Margolis, 1997.
Antigen Molecular Weight:
Human Protein Atlas:
Predicted Species Reactivity:  
Gene:
Immunogen: PBS-soluble chondroitin sulfate proteoglycans
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped:
Myeloma Strain: Designation:PA1
Epitope Location or Sequence:
Uniprot ID:
Immunogen Sequence:
Entrez Gene ID:
Additional Characterization:
Antibody Registry ID: AB_528397 
Additional Information: The origin of immunization is rat brain. During protocol 100 micrograms were used and complete freunds aduvant was used. RRID:AB_528397
Recommended Applications: Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
1D1 was deposited to the DSHB by Margolis, R.U. / Margolis, R.K. (DSHB Hybridoma Product 1D1)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

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