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SKU: 1F6
View product citations for antibody 1F6 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: neurocan (N-terminal epitope)
Hybridoma Cells Available: Yes
Antigen Species: rat
Depositor: Margolis, R.K. / Margolis, R.U.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: Department of Pharmacology State University of New York
Positive Tested Species Reactivity: Rat
Depositors Notes: For immunocytochemistry have used ascites at dilutions of 1:100-1:500. Hybridoma supernatant is most economical for use with immunoblots.
Antigen Molecular Weight:
Human Protein Atlas:  
Predicted Species Reactivity:  
Immunogen: PBS-soluble chondroitin sulfate proteoglycans
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: Designation:PA1
Epitope Location or Sequence:
Uniprot ID:  
Immunogen Sequence:
Entrez Gene ID:  
Additional Characterization:  
Antibody Registry ID: AB_2149837 
Additional Information: See Georgadaki and Zagris (2014) Research Journal of Developmental Biology Vol. 1 Article 3. Chmical properties can be found See Rauch et al., 1991, 1992; Meyer-Puttlitz et al., 1995, 1996; Margolis et al., 1996; Margolis and Margolis, 1997. RRID:AB_2149837
Recommended Applications: FFPE, Function Blocking, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
1F6 was deposited to the DSHB by Margolis, R.K. / Margolis, R.U. (DSHB Hybridoma Product 1F6)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

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