(0)No Reviews yet
$45.00 to $425.00
SKU: 1F6
View product citations for antibody 1F6 on CiteAb

In Stock

Available: 173

DSHB Data Sheet

Catalog Fields

Product Name/ID: 1F6
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Name:
Ab Isotype: MIgG1
Gene Symbol:
Antibody Registry ID: AB_2149837 
Uniprot ID:  
Entrez Gene ID:  
Clonality: Monoclonal
Immunogen: PBS-soluble chondroitin sulfate proteoglycans
Immunogen Sequence:
Myeloma Strain: Designation:PA1
Epitope Mapped: No
Antigen Name: neurocan (N-terminal epitope)
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 1/27/1998
Antigen Molecular Weight:
Depositor: Margolis, R.K. / Margolis, R.U.
Antigen Sequence:
Depositor Institution: New York University Medical Center / State University of New York
Antigen Species: rat
Depositor Notes: For immunocytochemistry have used ascites at dilutions of 1:100-1:500. Hybridoma supernatant is most economical for use with immunoblots.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Rat
Additional Information: See Georgadaki and Zagris (2014) Research Journal of Developmental Biology Vol. 1 Article 3. Chmical properties can be found See Rauch et al., 1991, 1992; Meyer-Puttlitz et al., 1995, 1996; Margolis et al., 1996; Margolis and Margolis, 1997. RRID:AB_2149837
Predicted Species Reactivity:  
Human Protein Atlas:  
Additional Characterization:  
Recommended Applications: FFPE, Function Blocking, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
1F6 was deposited to the DSHB by Margolis, R.K. / Margolis, R.U. (DSHB Hybridoma Product 1F6)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

7 References

  • Initial Publication
  • WB References
  • All References
  • Initial Publication
    WB References
    All References

    Ratings & Reviews

    No reviews available

    Be the first to Write a Review