1D9-E11

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SKU: 1D9-E11

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DSHB Data Sheet

Catalog Fields

Product Name/ID: 1D9-E11
Available to For-Profits: Yes
Alternate Antibody Name: 1D9
Gene Name:
Ab Isotype: MIgM, kappa light chain
Gene Symbol:
Antibody Registry ID: AB_2619607 
Uniprot ID:  
RRID:  
Entrez Gene ID:  
Clonality: Monoclonal
Immunogen: Formaldehyde-fixed cells from regenerating blastema of C1W4 clonal lineage
Clone:
Immunogen Sequence:
Myeloma Strain: F0
Epitope Mapped: No
Antigen Name: Regenerating blastema, brain primordia; neoblast progeny
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 8/28/2013
Antigen Molecular Weight:
Depositor: Zayas, R.M.
Antigen Sequence:
Depositor Institution: San Diego State University
Antigen Species: Schmidtea mediterranea
Depositor Notes: Strongly stains the regenerating blastema. This antibody labels a subset of unidentified cell nuclei distributed throughout the parenchyma (mesenchyme) with a concentration of positive nuclei near the cephalic ganglia and ventral nerve cords; a subset of labeled cells is gamma irradiation-sensitive.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Planaria, Schmidtea mediterranea
Additional Information:
Predicted Species Reactivity:  
Human Protein Atlas:  
Additional Characterization:  
Recommended Applications: Immunofluorescence
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
1D9-E11 was deposited to the DSHB by Zayas, R.M. (DSHB Hybridoma Product 1D9-E11)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

1 Reference

  • Initial Publication
  • IF References
  • All References
  • Initial Publication

    Novel monoclonal antibodies to study tissue regeneration in planarians.
    Zayas RM
    BMC developmental biology 15. (2015 Jan 21): 2.

    IF References

    Novel monoclonal antibodies to study tissue regeneration in planarians.
    Zayas RM
    BMC developmental biology 15. (2015 Jan 21): 2.

    All References

    Novel monoclonal antibodies to study tissue regeneration in planarians.
    Zayas RM
    BMC developmental biology 15. (2015 Jan 21): 2.

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