3C11 (anti SYNORF1)

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SKU: 3C11 (anti SYNORF1)
View product citations for antibody 3C11 (anti SYNORF1) on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: Synapsin
Hybridoma Cells Available: Yes
Antigen Species: Drosophila
Depositor: Buchner, E.
Isotype: MIgG2b, kappa light chain
Antigen Sequence:
Host Species: mouse
Depositors Institution: Inst. of Clinical Neurobiology, Universitaetsklinikim Wuerzburg
Positive Tested Species Reactivity: Cephalopod, Crustacean, Drosophila, Insect, Invertebrate, Leech, Mosquito, Moth, Planaria, Rat
Depositors Notes: Cross-reacts with many invertebrate species. Stains neuropil and synaptic active zones. 3C11 recognizes the synapsin-1 cleaved fragment. Immunostaining is improved when fixed with zinc-formaldehyde instead of formaldehyde which allows for greater penetration of the 3C11 mAb in whole-mount brains. See PMID: 18585788.
Antigen Molecular Weight: Apparent: multiple bands at 70, 74, 80, and 143kDa
Human Protein Atlas:
Predicted Species Reactivity:  
Gene: Syn
Immunogen: GST-Synapsin-GST fusion protein expressed in E. coli and purified by glutathione affinity.
Alternate Gene Names: Dsyn-1; syn; SYN; SYN1; SYN2; Syn-1; Syn-2
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: P3X63Ag8.653
Epitope Location or Sequence: Peptide sequence LFGGMEVCGL which lies in the conserved C domain.
Uniprot ID: Q24546 
Immunogen Sequence: SYNORF1 which corresponds to Synapsin-1 from Uniprot
Entrez Gene ID: 41247 
Additional Characterization:
Antibody Registry ID: AB_528479 
Additional Information: RRID: AB_528479
Recommended Applications: FFPE, Immunofluorescence, Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
3C11 (anti SYNORF1) was deposited to the DSHB by Buchner, E. (DSHB Hybridoma Product 3C11 (anti SYNORF1))
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

65 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • FFPE References
  • Epitope Map References
  • All References
  • Initial Publication
    IF References
    WB References
    IHC References
    FFPE References
    Epitope Map References
    All References

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