3C11 (anti SYNORF1)

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SKU: 3C11 (anti SYNORF1)

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Supernatant 1.0 ml Concentrate 0.1 ml Frozen Cells 1 vial Growing Cells 1 vial

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DSHB Data Sheet

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Product Name/ID: 3C11 (anti SYNORF1)

Available to For-Profits: Yes

Alternate Antibody Name:

Gene Name: Syn

Ab Isotype: MIgG2b, kappa light chain

Gene Symbol:

Antibody Registry ID: AB_528479

Uniprot ID: Q24546

RRID:

Entrez Gene ID: 41247

Clonality: Monoclonal

Immunogen: GST-Synapsin-GST fusion protein expressed in E. coli and purified by glutathione affinity.

Clone:

Immunogen Sequence: SYNORF1 which corresponds to Synapsin-1 from Uniprot

Myeloma Strain: P3X63Ag8.653

Epitope Mapped: Yes

Antigen Name: Synapsin

Epitope Location or Sequence: Peptide sequence LFGGMEVCGL which lies in the conserved C domain.

Alternate Antigen Name:

Deposit Date: 6/10/2005

Antigen Molecular Weight: Apparent: multiple bands at 70, 74, 80, and 143kDa

Depositor: Buchner, E.

Antigen Sequence:

Depositor Institution: Universitaetsklinikim Wuerzburg, Inst. of Clinical Neurobiology

Antigen Species: Drosophila

Depositor Notes: Cross-reacts with many invertebrate species. Stains neuropil and synaptic active zones. 3C11 recognizes the synapsin-1 cleaved fragment. Immunostaining is improved when fixed with zinc-formaldehyde instead of formaldehyde which allows for greater penetration of the 3C11 mAb in whole-mount brains. See PMID: 18585788.

Host Species: mouse

Hybridoma Cells Available (Non-Profit): Yes

Confirmed Species Reactivity: Cephalopod, Crustacean, Drosophila, Insect, Invertebrate, Leech, Mosquito, Moth, Planaria, Rat

Additional Information: RRID: AB_528479

Predicted Species Reactivity:

Human Protein Atlas:

Additional Characterization:

Recommended Applications: FFPE, Immunofluorescence, Immunohistochemistry, Western Blot

All cell products contain the antimicrobial ProClin. Click here for additional information.

These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.

For your Materials & Methods section:

3C11 (anti SYNORF1) was deposited to the DSHB by Buchner, E. (DSHB Hybridoma Product 3C11 (anti SYNORF1))

Storage and Handling Recommendations

Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.

Usage Recommendations

The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

70 References

Initial Publication

IF References

WB References

IHC References

FFPE References

Epitope Map References

All References

Initial Publication

Invertebrate synapsins: a single gene codes for several isoforms in Drosophila.
Buchner E
The Journal of neuroscience : the official journal of the Society for Neuroscience 16.10 (1996 May 15): 3154-65.

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3C11 (anti SYNORF1)

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