Hybridoma Cells Available: Yes
Antigen Species: Drosophila
Depositor: Buchner, E.
Isotype: MIgG2b, kappa light chain
Host Species: mouse
Depositors Institution: Inst. of Clinical Neurobiology, Universitaetsklinikim Wuerzburg
Positive Tested Species Reactivity: Cephalopod, Crustacean, Drosophila, Insect, Invertebrate, Leech, Mosquito, Moth, Planaria, Rat
Depositors Notes: Cross-reacts with many invertebrate species. Stains neuropil and synaptic active zones. 3C11 recognizes the synapsin-1 cleaved fragment. Immunostaining is improved when fixed with zinc-formaldehyde instead of formaldehyde which allows for greater penetration of the 3C11 mAb in whole-mount brains. See PMID: 18585788.
Antigen Molecular Weight: Apparent: multiple bands at 70, 74, 80, and 143kDa
Human Protein Atlas:
Predicted Species Reactivity:
Immunogen: GST-Synapsin-GST fusion protein expressed in E. coli and purified by glutathione affinity.
Alternate Gene Names: Dsyn-1; syn; SYN; SYN1; SYN2; Syn-1; Syn-2
Alternate Antibody Name:
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: P3X63Ag8.653
Epitope Location or Sequence: Peptide sequence LFGGMEVCGL which lies in the conserved C domain.
Uniprot ID: Q24546
Immunogen Sequence: SYNORF1 which corresponds to Synapsin-1 from Uniprot
Entrez Gene ID: 41247
Antibody Registry ID: AB_528479
Additional Information: RRID: AB_528479
Recommended Applications: FFPE, Immunofluorescence, Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
3C11 (anti SYNORF1) was deposited to the DSHB by Buchner, E. (DSHB Hybridoma Product 3C11 (anti SYNORF1))
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
Epitope Map References