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SKU: 20H5
View product citations for antibody 20H5 on CiteAb

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Available: 35

DSHB Data Sheet

Catalog Fields

Antigen: Cdk7, recombinant
Hybridoma Cells Available: Yes
Antigen Species: Drosophila
Depositor: Suter, B.
Isotype: MIgG1, kappa light chain
Depositors Institution: Institute of Cell Biology, University of Bern, Balzerstrasse
Host Species: mouse
Depositors Notes: Fixation of the immunostaining used 4% paraformaldehyde, epitope is methanol sensitive.
Positive Tested Species Reactivity: Drosophila
Clonality: Monoclonal
Antigen Molecular Weight: 40 kDa
Epitope Mapped: No
Immunogen: Cdk7 (mo15), recombinant Cdk7 expressed in E. coli with TiterMax¨ adjuvant
Additional Information: RRID:AB_2619579
Myeloma Strain: SP2/0
Antibody Registry ID: AB_2619579 
Recommended Applications: Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
20H5 was deposited to the DSHB by Suter, B. (DSHB Hybridoma Product 20H5)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

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