DCAD2

(0) No Reviews yet
$40.00
SKU: DCAD2
View product citations for antibody DCAD2 on CiteAb

In Stock

Available: 393

DSHB Data Sheet

Catalog Fields

Antigen: cadherin, DE- (extracellular domain)
Hybridoma Cells Available: Yes
Antigen Species: Drosophila
Depositor: Uemura, T.
Isotype: RIgG2a, kappa light chain
Depositors Institution: Kyoto Univ. Graduate Sch.of Biostudies
Host Species: rat
Depositors Notes: Suitable for use in immunostaining and immunoprecipitation. In western blot analysis, DCAD1, another rat monoclonal antibody to DE-cadherin, is more sensitive than DCAD2, although DCAD1 is not appropriate for staining. About requests of DCAD1, please contact Tadashi Uemura (tuemura@lif.kyoto-u.ac.jp). Conditions for immunostaining are described in our papers (Developmental Biology; Genes & Development 10, 659- 671); in addition please note the following comments. 1/ Fixatives (3.7% formaldehyde in PB or 4% paraformaldehyde in CMF & GBS containing 1mM CaCl2). Either fixation protocol works to stain embryos and discs. To stain cultured cells, only the 4% paraformaldehyde condition worked in our hands. We do not prefer any fix solutions including EDTA or EGTA, because cadherin is easily degraded under Ca++- free conditions. 2/ We suspect that the epitope may be sensitive to long exposure to alcohol. So, embryos stored in alcohol may not be stained. 3/ Photos should be taken under confocal microscope. 4/ HRP/DAB reaction has never given clear-cut signals for DE-cadherin at cell-cell contact sites.
Positive Tested Species Reactivity: Drosophila
Gene: shg
Antigen Molecular Weight: Predicted: 170 kDa; Apparent: 230 kDa [precursor] and 150 kDa [mature form]
Alternate Gene Names: CG3722, E-cadh
Immunogen: Proteins co-immunoprecipitated with an anti-alpha-catenin mAb, DCAT-1.
Clonality: Monoclonal
Myeloma Strain: P3-X63-Ag8-U1
Epitope Mapped: Yes
Uniprot ID: Q24298 
Epitope Location or Sequence: Between cadherin repeats 0 and 1
Entrez Gene ID: 37386 
Immunogen Sequence: Full length protein
Antibody Registry ID: AB_528120 
Additional Information: For experiments involving live cells and tissue, DCAD2 supernatant is available without the antimicrobial ProClin. Contact us at 319-335-3826 or dshb@uiowa.edu for special ordering instructions.
Recommended Applications: Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
DCAD2 was deposited to the DSHB by Uemura, T. (DSHB Hybridoma Product DCAD2)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

35 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • IP References
  • Epitope Map References
  • All References
  • Initial Publication
    IF References

    RhoL controls invasion and Rap1 localization during immune cell transmigration in Drosophila.
    Lehmann R
    Nature cell biology 12.6 (2010 Jun): 605-10.

    aPKC phosphorylation of Bazooka defines the apical/lateral border in Drosophila epithelial cells.
    St Johnston D
    Cell 141.3 (2010 Apr 30): 509-23.

    Neuralized promotes basal to apical transcytosis of delta in epithelial cells.
    Le Borgne R
    Molecular biology of the cell 21.12 (2010 Jun 15): 2078-86.

    Reiterative use of signalling pathways controls multiple cellular events during Drosophila posterior spiracle organogenesis.
    Graba Y
    Developmental biology 343.1-2 (2010 Jul 1): 18-27.

    Regulation of Hippo signaling by Jun kinase signaling during compensatory cell proliferation and regeneration, and in neoplastic tumors.
    Irvine KD
    Developmental biology 350.1 (2011 Feb 1): 139-51.

    Phosphorylation of Grainy head by ERK is essential for wound-dependent regeneration but not for development of an epidermal barrier.
    McGinnis W
    Proceedings of the National Academy of Sciences of the United States of America 108.2 (2011 Jan 11): 650-5.

    Planar polarized protrusions break the symmetry of EGFR signaling during Drosophila bract cell fate induction.
    Axelrod JD
    Developmental cell 23.3 (2012 Sep 11): 507-18.

    Zygotic Drosophila E-cadherin expression is required for processes of dynamic epithelial cell rearrangement in the Drosophila embryo.
    Takeichi M
    Genes & development 10.6 (1996 Mar 15): 659-71.

    Drosophila exocyst components Sec5, Sec6, and Sec15 regulate DE-Cadherin trafficking from recycling endosomes to the plasma membrane.
    Bellaïche Y
    Developmental cell 9.3 (2005 Sep): 365-76.

    Nonchordate classic cadherins have a structurally and functionally unique domain that is absent from chordate classic cadherins.
    Tsukita S
    Developmental biology 216.1 (1999 Dec 1): 406-22.

    Dynamic features of adherens junctions during Drosophila embryonic epithelial morphogenesis revealed by a Dalpha-catenin-GFP fusion protein.
    Tsukita S
    Development genes and evolution 209.4 (1999 Apr): 218-25.

    Essential requirement for RING finger E3 ubiquitin ligase Hakai in early embryonic development of Drosophila.
    Hayashi S
    Genes to cells : devoted to molecular & cellular mechanisms 14.9 (2009 Sep): 1067-77.

    Lissencephaly-1 controls germline stem cell self-renewal through modulating bone morphogenetic protein signaling and niche adhesion.
    Xie T
    Proceedings of the National Academy of Sciences of the United States of America 107.46 (2010 Nov 16): 19939-44.

    Binding site for p120/delta-catenin is not required for Drosophila E-cadherin function in vivo.
    Rorth P
    The Journal of cell biology 160.3 (2003 Feb 3): 313-9.

    The Bro1-domain-containing protein Myopic/HDPTP coordinates with Rab4 to regulate cell adhesion and migration.
    Chen GC
    Journal of cell science 125.Pt 20 (2012 Oct 15): 4841-52.

    Specific association of Piwi with rasiRNAs derived from retrotransposon and heterochromatic regions in the Drosophila genome.
    Siomi MC
    Genes & development 20.16 (2006 Aug 15): 2214-22.

    dachshund Potentiates Hedgehog Signaling during Drosophila Retinogenesis.
    Janody F
    PLoS genetics 12.7 (2016 Jul): e1006204.

    Long Term Ex Vivo Culture and Live Imaging of Drosophila Larval Imaginal Discs.
    Sun YH
    PloS one 11.9 (2016): e0163744.

    Mutant analysis by rescue gene excision: New tools for mosaic studies in Drosophila.
    Pignoni F
    Genesis (New York, N.Y. : 2000) 54.11 (2016 Nov): 589-592.

    Quantitative microscopy of the Drosophila ovary shows multiple niche signals specify progenitor cell fate.
    Montell DJ
    Nature communications 8.1 (2017 Nov 1): 1244.

    Feedback amplification loop drives malignant growth in epithelial tissues.
    Milán M
    Proceedings of the National Academy of Sciences of the United States of America 114.35 (2017 Aug 29): E7291-E7300.

    Myosin II is not required for <i>Drosophila</i> tracheal branch elongation and cell intercalation.
    Affolter M
    Development (Cambridge, England) 144.16 (2017 Aug 15): 2961-2968.

    C-terminal Src kinase (Csk) regulates the tricellular junction protein Gliotactin independent of Src.
    Auld VJ
    Molecular biology of the cell 29.2 (2018 Jan 15): 123-136.

    Glass promotes the differentiation of neuronal and non-neuronal cell types in the Drosophila eye.
    Treisman JE
    PLoS genetics 14.1 (2018 Jan): e1007173.

    The Misshapen kinase regulates the size and stability of the germline ring canals in the Drosophila egg chamber.
    Lewellyn L
    Developmental biology 440.2 (2018 Aug 15): 99-112.

    Drosophila TNF Modulates Tissue Tension in the Embryo to Facilitate Macrophage Invasive Migration.
    Siekhaus DE
    Developmental cell 45.3 (2018 May 7): 331-346.e7.

    Differential lateral and basal tension drive folding of Drosophila wing discs through two distinct mechanisms.
    Dahmann C
    Nature communications 9.1 (2018 Nov 5): 4620.

    Establishment and characterization of Drosophila cell lines mutant for heparan sulfate modifying enzymes.
    Nakato H
    Glycobiology 29.6 (2019 Jun 1): 479-489.

    WB References
    IHC References
    IP References
    Epitope Map References
    All References

    Increased H⁺ efflux is sufficient to induce dysplasia and necessary for viability with oncogene expression.
    Barber DL
    eLife 4. (2015 Mar 20): .

    The Tricellular Junction Protein Sidekick Regulates Vertex Dynamics to Promote Bicellular Junction Extension.
    Kuranaga E
    Developmental cell 50.3 (2019 Aug 5): 327-338.e5.

    Sidekick Is a Key Component of Tricellular Adherens Junctions that Acts to Resolve Cell Rearrangements.
    Treisman JE
    Developmental cell 50.3 (2019 Aug 5): 313-326.e5.

    Integrins are required for synchronous ommatidial rotation in the Drosophila eye linking planar cell polarity signalling to the extracellular matrix.
    Mlodzik M
    Open biology 9.8 (2019 Aug 30): 190148.

    RhoL controls invasion and Rap1 localization during immune cell transmigration in Drosophila.
    Lehmann R
    Nature cell biology 12.6 (2010 Jun): 605-10.

    aPKC phosphorylation of Bazooka defines the apical/lateral border in Drosophila epithelial cells.
    St Johnston D
    Cell 141.3 (2010 Apr 30): 509-23.

    Neuralized promotes basal to apical transcytosis of delta in epithelial cells.
    Le Borgne R
    Molecular biology of the cell 21.12 (2010 Jun 15): 2078-86.

    Reiterative use of signalling pathways controls multiple cellular events during Drosophila posterior spiracle organogenesis.
    Graba Y
    Developmental biology 343.1-2 (2010 Jul 1): 18-27.

    Regulation of Hippo signaling by Jun kinase signaling during compensatory cell proliferation and regeneration, and in neoplastic tumors.
    Irvine KD
    Developmental biology 350.1 (2011 Feb 1): 139-51.

    Phosphorylation of Grainy head by ERK is essential for wound-dependent regeneration but not for development of an epidermal barrier.
    McGinnis W
    Proceedings of the National Academy of Sciences of the United States of America 108.2 (2011 Jan 11): 650-5.

    Planar polarized protrusions break the symmetry of EGFR signaling during Drosophila bract cell fate induction.
    Axelrod JD
    Developmental cell 23.3 (2012 Sep 11): 507-18.

    Zygotic Drosophila E-cadherin expression is required for processes of dynamic epithelial cell rearrangement in the Drosophila embryo.
    Takeichi M
    Genes & development 10.6 (1996 Mar 15): 659-71.

    Drosophila exocyst components Sec5, Sec6, and Sec15 regulate DE-Cadherin trafficking from recycling endosomes to the plasma membrane.
    Bellaïche Y
    Developmental cell 9.3 (2005 Sep): 365-76.

    Nonchordate classic cadherins have a structurally and functionally unique domain that is absent from chordate classic cadherins.
    Tsukita S
    Developmental biology 216.1 (1999 Dec 1): 406-22.

    Dynamic features of adherens junctions during Drosophila embryonic epithelial morphogenesis revealed by a Dalpha-catenin-GFP fusion protein.
    Tsukita S
    Development genes and evolution 209.4 (1999 Apr): 218-25.

    Essential requirement for RING finger E3 ubiquitin ligase Hakai in early embryonic development of Drosophila.
    Hayashi S
    Genes to cells : devoted to molecular & cellular mechanisms 14.9 (2009 Sep): 1067-77.

    Lissencephaly-1 controls germline stem cell self-renewal through modulating bone morphogenetic protein signaling and niche adhesion.
    Xie T
    Proceedings of the National Academy of Sciences of the United States of America 107.46 (2010 Nov 16): 19939-44.

    Binding site for p120/delta-catenin is not required for Drosophila E-cadherin function in vivo.
    Rorth P
    The Journal of cell biology 160.3 (2003 Feb 3): 313-9.

    The Bro1-domain-containing protein Myopic/HDPTP coordinates with Rab4 to regulate cell adhesion and migration.
    Chen GC
    Journal of cell science 125.Pt 20 (2012 Oct 15): 4841-52.

    Specific association of Piwi with rasiRNAs derived from retrotransposon and heterochromatic regions in the Drosophila genome.
    Siomi MC
    Genes & development 20.16 (2006 Aug 15): 2214-22.

    dachshund Potentiates Hedgehog Signaling during Drosophila Retinogenesis.
    Janody F
    PLoS genetics 12.7 (2016 Jul): e1006204.

    Long Term Ex Vivo Culture and Live Imaging of Drosophila Larval Imaginal Discs.
    Sun YH
    PloS one 11.9 (2016): e0163744.

    Mutant analysis by rescue gene excision: New tools for mosaic studies in Drosophila.
    Pignoni F
    Genesis (New York, N.Y. : 2000) 54.11 (2016 Nov): 589-592.

    Quantitative microscopy of the Drosophila ovary shows multiple niche signals specify progenitor cell fate.
    Montell DJ
    Nature communications 8.1 (2017 Nov 1): 1244.

    Feedback amplification loop drives malignant growth in epithelial tissues.
    Milán M
    Proceedings of the National Academy of Sciences of the United States of America 114.35 (2017 Aug 29): E7291-E7300.

    Myosin II is not required for <i>Drosophila</i> tracheal branch elongation and cell intercalation.
    Affolter M
    Development (Cambridge, England) 144.16 (2017 Aug 15): 2961-2968.

    C-terminal Src kinase (Csk) regulates the tricellular junction protein Gliotactin independent of Src.
    Auld VJ
    Molecular biology of the cell 29.2 (2018 Jan 15): 123-136.

    Glass promotes the differentiation of neuronal and non-neuronal cell types in the Drosophila eye.
    Treisman JE
    PLoS genetics 14.1 (2018 Jan): e1007173.

    The Misshapen kinase regulates the size and stability of the germline ring canals in the Drosophila egg chamber.
    Lewellyn L
    Developmental biology 440.2 (2018 Aug 15): 99-112.

    Drosophila TNF Modulates Tissue Tension in the Embryo to Facilitate Macrophage Invasive Migration.
    Siekhaus DE
    Developmental cell 45.3 (2018 May 7): 331-346.e7.

    Differential lateral and basal tension drive folding of Drosophila wing discs through two distinct mechanisms.
    Dahmann C
    Nature communications 9.1 (2018 Nov 5): 4620.

    Establishment and characterization of Drosophila cell lines mutant for heparan sulfate modifying enzymes.
    Nakato H
    Glycobiology 29.6 (2019 Jun 1): 479-489.

    A Drosophila homolog of cadherin associated with armadillo and essential for embryonic cell-cell adhesion.
    Takeichi M
    Developmental biology 165.2 (1994 Oct): 716-26.

    Characterization of mouse dishevelled (Dvl) proteins in Wnt/Wingless signaling pathway.
    Yanagawa S
    The Journal of biological chemistry 274.30 (1999 Jul 23): 21464-70.

    Axon patterning requires DN-cadherin, a novel neuronal adhesion receptor, in the Drosophila embryonic CNS.
    Uemura T
    Neuron 19.1 (1997 Jul): 77-89.

    Ratings & Reviews

    No reviews available

    Be the first to Write a Review