DCAD2

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$45.00 to $425.00
SKU: DCAD2
View product citations for antibody DCAD2 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: cadherin, DE- (extracellular domain)
Hybridoma Cells Available: Yes
Antigen Species: Drosophila
Depositor: Uemura, T.
Isotype: RIgG2a, kappa light chain
Antigen Sequence:
Host Species: rat
Depositors Institution: Kyoto Univ. Graduate Sch.of Biostudies
Positive Tested Species Reactivity: Drosophila
Depositors Notes: Suitable for use in immunostaining and immunoprecipitation. In western blot analysis, DCAD1, another rat monoclonal antibody to DE-cadherin, is more sensitive than DCAD2, although DCAD1 is not appropriate for staining. About requests of DCAD1, please contact Tadashi Uemura (tuemura@lif.kyoto-u.ac.jp). Conditions for immunostaining are described in our papers (Developmental Biology; Genes & Development 10, 659- 671); in addition please note the following comments. 1/ Fixatives (3.7% formaldehyde in PB or 4% paraformaldehyde in CMF & GBS containing 1mM CaCl2). Either fixation protocol works to stain embryos and discs. To stain cultured cells, only the 4% paraformaldehyde condition worked in our hands. We do not prefer any fix solutions including EDTA or EGTA, because cadherin is easily degraded under Ca++- free conditions. 2/ We suspect that the epitope may be sensitive to long exposure to alcohol. So, embryos stored in alcohol may not be stained. 3/ Photos should be taken under confocal microscope. 4/ HRP/DAB reaction has never given clear-cut signals for DE-cadherin at cell-cell contact sites.
Antigen Molecular Weight: Predicted: 170 kDa; Apparent: 230 kDa [precursor] and 150 kDa [mature form]
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: shg
Immunogen: Proteins co-immunoprecipitated with an anti-alpha-catenin mAb, DCAT-1.
Alternate Gene Names: CG3722, E-cadh
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: P3-X63-Ag8-U1
Epitope Location or Sequence: Between cadherin repeats 0 and 1
Uniprot ID: Q24298 
Immunogen Sequence: Full length protein
Entrez Gene ID: 37386 
Additional Characterization:  
Antibody Registry ID: AB_528120 
Additional Information:
Recommended Applications: Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
DCAD2 was deposited to the DSHB by Uemura, T. (DSHB Hybridoma Product DCAD2)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
All cell products contain the antimicrobial ProClin. Click here for additional information.

47 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • IP References
  • Epitope Map References
  • All References
  • Initial Publication
    IF References

    RhoL controls invasion and Rap1 localization during immune cell transmigration in Drosophila.
    Lehmann R
    Nature cell biology 12.6 (2010 Jun): 605-10.

    aPKC phosphorylation of Bazooka defines the apical/lateral border in Drosophila epithelial cells.
    St Johnston D
    Cell 141.3 (2010 Apr 30): 509-23.

    Neuralized promotes basal to apical transcytosis of delta in epithelial cells.
    Le Borgne R
    Molecular biology of the cell 21.12 (2010 Jun 15): 2078-86.

    Reiterative use of signalling pathways controls multiple cellular events during Drosophila posterior spiracle organogenesis.
    Graba Y
    Developmental biology 343.1-2 (2010 Jul 1): 18-27.

    Regulation of Hippo signaling by Jun kinase signaling during compensatory cell proliferation and regeneration, and in neoplastic tumors.
    Irvine KD
    Developmental biology 350.1 (2011 Feb 1): 139-51.

    Phosphorylation of Grainy head by ERK is essential for wound-dependent regeneration but not for development of an epidermal barrier.
    McGinnis W
    Proceedings of the National Academy of Sciences of the United States of America 108.2 (2011 Jan 11): 650-5.

    Planar polarized protrusions break the symmetry of EGFR signaling during Drosophila bract cell fate induction.
    Axelrod JD
    Developmental cell 23.3 (2012 Sep 11): 507-18.

    Zygotic Drosophila E-cadherin expression is required for processes of dynamic epithelial cell rearrangement in the Drosophila embryo.
    Takeichi M
    Genes & development 10.6 (1996 Mar 15): 659-71.

    Drosophila exocyst components Sec5, Sec6, and Sec15 regulate DE-Cadherin trafficking from recycling endosomes to the plasma membrane.
    Bellaïche Y
    Developmental cell 9.3 (2005 Sep): 365-76.

    Nonchordate classic cadherins have a structurally and functionally unique domain that is absent from chordate classic cadherins.
    Tsukita S
    Developmental biology 216.1 (1999 Dec 1): 406-22.

    Dynamic features of adherens junctions during Drosophila embryonic epithelial morphogenesis revealed by a Dalpha-catenin-GFP fusion protein.
    Tsukita S
    Development genes and evolution 209.4 (1999 Apr): 218-25.

    Essential requirement for RING finger E3 ubiquitin ligase Hakai in early embryonic development of Drosophila.
    Hayashi S
    Genes to cells : devoted to molecular & cellular mechanisms 14.9 (2009 Sep): 1067-77.

    Lissencephaly-1 controls germline stem cell self-renewal through modulating bone morphogenetic protein signaling and niche adhesion.
    Xie T
    Proceedings of the National Academy of Sciences of the United States of America 107.46 (2010 Nov 16): 19939-44.

    Binding site for p120/delta-catenin is not required for Drosophila E-cadherin function in vivo.
    Rorth P
    The Journal of cell biology 160.3 (2003 Feb 3): 313-9.

    The Bro1-domain-containing protein Myopic/HDPTP coordinates with Rab4 to regulate cell adhesion and migration.
    Chen GC
    Journal of cell science 125.Pt 20 (2012 Oct 15): 4841-52.

    Specific association of Piwi with rasiRNAs derived from retrotransposon and heterochromatic regions in the Drosophila genome.
    Siomi MC
    Genes & development 20.16 (2006 Aug 15): 2214-22.

    dachshund Potentiates Hedgehog Signaling during Drosophila Retinogenesis.
    Janody F
    PLoS genetics 12.7 (2016 Jul): e1006204.

    Long Term Ex Vivo Culture and Live Imaging of Drosophila Larval Imaginal Discs.
    Sun YH
    PloS one 11.9 (2016): e0163744.

    Mutant analysis by rescue gene excision: New tools for mosaic studies in Drosophila.
    Pignoni F
    Genesis (New York, N.Y. : 2000) 54.11 (2016 Nov): 589-592.

    Quantitative microscopy of the Drosophila ovary shows multiple niche signals specify progenitor cell fate.
    Montell DJ
    Nature communications 8.1 (2017 Nov 1): 1244.

    Feedback amplification loop drives malignant growth in epithelial tissues.
    Milán M
    Proceedings of the National Academy of Sciences of the United States of America 114.35 (2017 Aug 29): E7291-E7300.

    Myosin II is not required for <i>Drosophila</i> tracheal branch elongation and cell intercalation.
    Affolter M
    Development (Cambridge, England) 144.16 (2017 Aug 15): 2961-2968.

    C-terminal Src kinase (Csk) regulates the tricellular junction protein Gliotactin independent of Src.
    Auld VJ
    Molecular biology of the cell 29.2 (2018 Jan 15): 123-136.

    Glass promotes the differentiation of neuronal and non-neuronal cell types in the Drosophila eye.
    Treisman JE
    PLoS genetics 14.1 (2018 Jan): e1007173.

    The Misshapen kinase regulates the size and stability of the germline ring canals in the Drosophila egg chamber.
    Lewellyn L
    Developmental biology 440.2 (2018 Aug 15): 99-112.

    Drosophila TNF Modulates Tissue Tension in the Embryo to Facilitate Macrophage Invasive Migration.
    Siekhaus DE
    Developmental cell 45.3 (2018 May 7): 331-346.e7.

    Differential lateral and basal tension drive folding of Drosophila wing discs through two distinct mechanisms.
    Dahmann C
    Nature communications 9.1 (2018 Nov 5): 4620.

    Establishment and characterization of Drosophila cell lines mutant for heparan sulfate modifying enzymes.
    Nakato H
    Glycobiology 29.6 (2019 Jun 1): 479-489.

    Cellular homeostasis in the <i>Drosophila</i> retina requires the lipid phosphatase Sac1.
    Brill JA
    Molecular biology of the cell 31.11 (2020 May 15): 1183-1199.

    Integration of Migratory Cells into a New Site In Vivo Requires Channel-Independent Functions of Innexins on Microtubules.
    Montell DJ
    Developmental cell 54.4 (2020 Aug 24): 501-515.e9.

    Hormone receptor 4 is required in muscles and distinct ovarian cell types to regulate specific steps of <i>Drosophila</i> oogenesis.
    Drummond-Barbosa D
    Development (Cambridge, England) 148.5 (2021 Mar 9): .

    The GTPase Rab8 differentially controls the long- and short-range activity of the Hedgehog morphogen gradient by regulating Hedgehog apico-basal distribution.
    Thérond PP
    Development (Cambridge, England) 148.5 (2021 Mar 9): .

    Enabled/VASP is required to mediate proper sealing of opposing cardioblasts during Drosophila dorsal vessel formation.
    Kramer SG
    Developmental dynamics : an official publication of the American Association of Anatomists 250.8 (2021 Aug): 1173-1190.

    Merlin and expanded integrate cell signaling that regulates cyst stem cell proliferation in the Drosophila testis niche.
    Leatherman J
    Developmental biology 477. (2021 Sep): 133-144.

    Interaction between Ras and Src clones causes interdependent tumor malignancy via Notch signaling in Drosophila.
    Igaki T
    Developmental cell 56.15 (2021 Aug 9): 2223-2236.e5.

    WB References
    IHC References

    Increased H⁺ efflux is sufficient to induce dysplasia and necessary for viability with oncogene expression.
    Barber DL
    eLife 4. (2015 Mar 20): .

    The Tricellular Junction Protein Sidekick Regulates Vertex Dynamics to Promote Bicellular Junction Extension.
    Kuranaga E
    Developmental cell 50.3 (2019 Aug 5): 327-338.e5.

    Sidekick Is a Key Component of Tricellular Adherens Junctions that Acts to Resolve Cell Rearrangements.
    Treisman JE
    Developmental cell 50.3 (2019 Aug 5): 313-326.e5.

    Integrins are required for synchronous ommatidial rotation in the Drosophila eye linking planar cell polarity signalling to the extracellular matrix.
    Mlodzik M
    Open biology 9.8 (2019 Aug 30): 190148.

    A Forward Genetic Approach to Mapping a <i>P</i>-Element Second Site Mutation Identifies <i>DCP2</i> as a Novel Tumor Suppressor in <i>Drosophila melanogaster</i>.
    Roy JK
    G3 (Bethesda, Md.) 10.8 (2020 Aug 5): 2601-2618.

    Context-Dependent Tumorigenic Effect of Testis-Specific Mitochondrial Protein <i>Tiny Tim 2</i> in Drosophila Somatic Epithelia.
    Gonzalez C
    Cells 9.8 (2020 Aug 6): .

    Transcriptomic analysis of feminizing somatic stem cells in the Drosophila testis reveals putative downstream effectors of the transcription factor Chinmo.
    Bach EA
    G3 (Bethesda, Md.) 11.4 (2021 Apr 15): .

    Ubx-Collier signaling cascade maintains blood progenitors in the posterior lobes of the Drosophila larval lymph gland.
    Mandal L
    PLoS genetics 17.8 (2021 Aug): e1009709.

    brinker levels regulated by a promoter proximal element support germ cell homeostasis.
    Stathopoulos A
    Development (Cambridge, England) 149.3 (2022 Feb 1): .

    IP References
    Epitope Map References
    All References

    Increased H⁺ efflux is sufficient to induce dysplasia and necessary for viability with oncogene expression.
    Barber DL
    eLife 4. (2015 Mar 20): .

    The Tricellular Junction Protein Sidekick Regulates Vertex Dynamics to Promote Bicellular Junction Extension.
    Kuranaga E
    Developmental cell 50.3 (2019 Aug 5): 327-338.e5.

    Sidekick Is a Key Component of Tricellular Adherens Junctions that Acts to Resolve Cell Rearrangements.
    Treisman JE
    Developmental cell 50.3 (2019 Aug 5): 313-326.e5.

    Integrins are required for synchronous ommatidial rotation in the Drosophila eye linking planar cell polarity signalling to the extracellular matrix.
    Mlodzik M
    Open biology 9.8 (2019 Aug 30): 190148.

    A Forward Genetic Approach to Mapping a <i>P</i>-Element Second Site Mutation Identifies <i>DCP2</i> as a Novel Tumor Suppressor in <i>Drosophila melanogaster</i>.
    Roy JK
    G3 (Bethesda, Md.) 10.8 (2020 Aug 5): 2601-2618.

    Context-Dependent Tumorigenic Effect of Testis-Specific Mitochondrial Protein <i>Tiny Tim 2</i> in Drosophila Somatic Epithelia.
    Gonzalez C
    Cells 9.8 (2020 Aug 6): .

    Transcriptomic analysis of feminizing somatic stem cells in the Drosophila testis reveals putative downstream effectors of the transcription factor Chinmo.
    Bach EA
    G3 (Bethesda, Md.) 11.4 (2021 Apr 15): .

    Ubx-Collier signaling cascade maintains blood progenitors in the posterior lobes of the Drosophila larval lymph gland.
    Mandal L
    PLoS genetics 17.8 (2021 Aug): e1009709.

    brinker levels regulated by a promoter proximal element support germ cell homeostasis.
    Stathopoulos A
    Development (Cambridge, England) 149.3 (2022 Feb 1): .

    RhoL controls invasion and Rap1 localization during immune cell transmigration in Drosophila.
    Lehmann R
    Nature cell biology 12.6 (2010 Jun): 605-10.

    aPKC phosphorylation of Bazooka defines the apical/lateral border in Drosophila epithelial cells.
    St Johnston D
    Cell 141.3 (2010 Apr 30): 509-23.

    Neuralized promotes basal to apical transcytosis of delta in epithelial cells.
    Le Borgne R
    Molecular biology of the cell 21.12 (2010 Jun 15): 2078-86.

    Reiterative use of signalling pathways controls multiple cellular events during Drosophila posterior spiracle organogenesis.
    Graba Y
    Developmental biology 343.1-2 (2010 Jul 1): 18-27.

    Regulation of Hippo signaling by Jun kinase signaling during compensatory cell proliferation and regeneration, and in neoplastic tumors.
    Irvine KD
    Developmental biology 350.1 (2011 Feb 1): 139-51.

    Phosphorylation of Grainy head by ERK is essential for wound-dependent regeneration but not for development of an epidermal barrier.
    McGinnis W
    Proceedings of the National Academy of Sciences of the United States of America 108.2 (2011 Jan 11): 650-5.

    Planar polarized protrusions break the symmetry of EGFR signaling during Drosophila bract cell fate induction.
    Axelrod JD
    Developmental cell 23.3 (2012 Sep 11): 507-18.

    Zygotic Drosophila E-cadherin expression is required for processes of dynamic epithelial cell rearrangement in the Drosophila embryo.
    Takeichi M
    Genes & development 10.6 (1996 Mar 15): 659-71.

    Drosophila exocyst components Sec5, Sec6, and Sec15 regulate DE-Cadherin trafficking from recycling endosomes to the plasma membrane.
    Bellaïche Y
    Developmental cell 9.3 (2005 Sep): 365-76.

    Nonchordate classic cadherins have a structurally and functionally unique domain that is absent from chordate classic cadherins.
    Tsukita S
    Developmental biology 216.1 (1999 Dec 1): 406-22.

    Dynamic features of adherens junctions during Drosophila embryonic epithelial morphogenesis revealed by a Dalpha-catenin-GFP fusion protein.
    Tsukita S
    Development genes and evolution 209.4 (1999 Apr): 218-25.

    Essential requirement for RING finger E3 ubiquitin ligase Hakai in early embryonic development of Drosophila.
    Hayashi S
    Genes to cells : devoted to molecular & cellular mechanisms 14.9 (2009 Sep): 1067-77.

    Lissencephaly-1 controls germline stem cell self-renewal through modulating bone morphogenetic protein signaling and niche adhesion.
    Xie T
    Proceedings of the National Academy of Sciences of the United States of America 107.46 (2010 Nov 16): 19939-44.

    Binding site for p120/delta-catenin is not required for Drosophila E-cadherin function in vivo.
    Rorth P
    The Journal of cell biology 160.3 (2003 Feb 3): 313-9.

    The Bro1-domain-containing protein Myopic/HDPTP coordinates with Rab4 to regulate cell adhesion and migration.
    Chen GC
    Journal of cell science 125.Pt 20 (2012 Oct 15): 4841-52.

    Specific association of Piwi with rasiRNAs derived from retrotransposon and heterochromatic regions in the Drosophila genome.
    Siomi MC
    Genes & development 20.16 (2006 Aug 15): 2214-22.

    dachshund Potentiates Hedgehog Signaling during Drosophila Retinogenesis.
    Janody F
    PLoS genetics 12.7 (2016 Jul): e1006204.

    Long Term Ex Vivo Culture and Live Imaging of Drosophila Larval Imaginal Discs.
    Sun YH
    PloS one 11.9 (2016): e0163744.

    Mutant analysis by rescue gene excision: New tools for mosaic studies in Drosophila.
    Pignoni F
    Genesis (New York, N.Y. : 2000) 54.11 (2016 Nov): 589-592.

    Quantitative microscopy of the Drosophila ovary shows multiple niche signals specify progenitor cell fate.
    Montell DJ
    Nature communications 8.1 (2017 Nov 1): 1244.

    Feedback amplification loop drives malignant growth in epithelial tissues.
    Milán M
    Proceedings of the National Academy of Sciences of the United States of America 114.35 (2017 Aug 29): E7291-E7300.

    Myosin II is not required for <i>Drosophila</i> tracheal branch elongation and cell intercalation.
    Affolter M
    Development (Cambridge, England) 144.16 (2017 Aug 15): 2961-2968.

    C-terminal Src kinase (Csk) regulates the tricellular junction protein Gliotactin independent of Src.
    Auld VJ
    Molecular biology of the cell 29.2 (2018 Jan 15): 123-136.

    Glass promotes the differentiation of neuronal and non-neuronal cell types in the Drosophila eye.
    Treisman JE
    PLoS genetics 14.1 (2018 Jan): e1007173.

    The Misshapen kinase regulates the size and stability of the germline ring canals in the Drosophila egg chamber.
    Lewellyn L
    Developmental biology 440.2 (2018 Aug 15): 99-112.

    Drosophila TNF Modulates Tissue Tension in the Embryo to Facilitate Macrophage Invasive Migration.
    Siekhaus DE
    Developmental cell 45.3 (2018 May 7): 331-346.e7.

    Differential lateral and basal tension drive folding of Drosophila wing discs through two distinct mechanisms.
    Dahmann C
    Nature communications 9.1 (2018 Nov 5): 4620.

    Establishment and characterization of Drosophila cell lines mutant for heparan sulfate modifying enzymes.
    Nakato H
    Glycobiology 29.6 (2019 Jun 1): 479-489.

    Cellular homeostasis in the <i>Drosophila</i> retina requires the lipid phosphatase Sac1.
    Brill JA
    Molecular biology of the cell 31.11 (2020 May 15): 1183-1199.

    Integration of Migratory Cells into a New Site In Vivo Requires Channel-Independent Functions of Innexins on Microtubules.
    Montell DJ
    Developmental cell 54.4 (2020 Aug 24): 501-515.e9.

    Hormone receptor 4 is required in muscles and distinct ovarian cell types to regulate specific steps of <i>Drosophila</i> oogenesis.
    Drummond-Barbosa D
    Development (Cambridge, England) 148.5 (2021 Mar 9): .

    The GTPase Rab8 differentially controls the long- and short-range activity of the Hedgehog morphogen gradient by regulating Hedgehog apico-basal distribution.
    Thérond PP
    Development (Cambridge, England) 148.5 (2021 Mar 9): .

    Enabled/VASP is required to mediate proper sealing of opposing cardioblasts during Drosophila dorsal vessel formation.
    Kramer SG
    Developmental dynamics : an official publication of the American Association of Anatomists 250.8 (2021 Aug): 1173-1190.

    Merlin and expanded integrate cell signaling that regulates cyst stem cell proliferation in the Drosophila testis niche.
    Leatherman J
    Developmental biology 477. (2021 Sep): 133-144.

    Interaction between Ras and Src clones causes interdependent tumor malignancy via Notch signaling in Drosophila.
    Igaki T
    Developmental cell 56.15 (2021 Aug 9): 2223-2236.e5.

    A Drosophila homolog of cadherin associated with armadillo and essential for embryonic cell-cell adhesion.
    Takeichi M
    Developmental biology 165.2 (1994 Oct): 716-26.

    Characterization of mouse dishevelled (Dvl) proteins in Wnt/Wingless signaling pathway.
    Yanagawa S
    The Journal of biological chemistry 274.30 (1999 Jul 23): 21464-70.

    Axon patterning requires DN-cadherin, a novel neuronal adhesion receptor, in the Drosophila embryonic CNS.
    Uemura T
    Neuron 19.1 (1997 Jul): 77-89.

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