DCAD2

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SKU: DCAD2

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Supernatant 1.0 ml Concentrate 0.1 ml Frozen Cells 1 vial Growing Cells 1 vial

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DSHB Data Sheet

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Clone ID/Product Name: DCAD2

Available to For-Profits: Yes

Alternate Antibody Name:

Gene Symbol: shg

Ab Isotype: RIgG2a, kappa light chain

Gene Name:

Antibody Registry ID: AB_528120

Uniprot ID: Q24298

RRID:

Entrez Gene ID: 37386

Clonality: Monoclonal

Immunogen: Proteins co-immunoprecipitated with an anti-alpha-catenin mAb, DCAT-1.

Clone:

Immunogen Sequence: Full length protein

Myeloma Strain: P3-X63-Ag8-U1

Epitope Mapped: Yes

Antigen Name: cadherin, DE- (extracellular domain)

Epitope Location or Sequence: Between cadherin repeats 0 and 1

Alternate Antigen Name:

Deposit Date: 1/7/2004

Antigen Molecular Weight: Predicted: 170 kDa; Apparent: 230 kDa [precursor] and 150 kDa [mature form]

Depositor: Uemura, T.

Antigen Sequence:

Depositor Institution: Kyoto Univ. Graduate Sch.of Biostudies

Antigen Species: Drosophila

Depositor Notes: Suitable for use in immunostaining and immunoprecipitation. In western blot analysis, DCAD1, another rat monoclonal antibody to DE-cadherin, is more sensitive than DCAD2, although DCAD1 is not appropriate for staining. About requests of DCAD1, please contact Tadashi Uemura (tuemura@lif.kyoto-u.ac.jp). Conditions for immunostaining are described in our papers (Developmental Biology; Genes & Development 10, 659- 671); in addition please note the following comments. 1/ Fixatives (3.7% formaldehyde in PB or 4% paraformaldehyde in CMF & GBS containing 1mM CaCl2). Either fixation protocol works to stain embryos and discs. To stain cultured cells, only the 4% paraformaldehyde condition worked in our hands. We do not prefer any fix solutions including EDTA or EGTA, because cadherin is easily degraded under Ca++- free conditions. 2/ We suspect that the epitope may be sensitive to long exposure to alcohol. So, embryos stored in alcohol may not be stained. 3/ Photos should be taken under confocal microscope. 4/ HRP/DAB reaction has never given clear-cut signals for DE-cadherin at cell-cell contact sites.

Host Species: rat

Hybridoma Cells Available (Non-Profit): Yes

Confirmed Species Reactivity: Drosophila

Additional Information:

Predicted Species Reactivity:

Human Protein Atlas:

Additional Characterization:

Recommended Applications: Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot

All cell products contain the antimicrobial ProClin. Click here for additional information.

These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.

For your Materials & Methods section:

DCAD2 was deposited to the DSHB by Uemura, T. (DSHB Hybridoma Product DCAD2)

Storage and Handling Recommendations

Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.

Usage Recommendations

The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

48 References

Initial Publication

IF References

WB References

IHC References

IP References

Epitope Map References

All References

Initial Publication

A Drosophila homolog of cadherin associated with armadillo and essential for embryonic cell-cell adhesion.
Takeichi M
Developmental biology 165.2 (1994 Oct): 716-26.

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DCAD2

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