Antigen: ATPase, (Na(+) K(+)) beta-subunit
Hybridoma Cells Available: Yes
Antigen Species: chicken
Depositor: Fambrough, D.M.
Host Species: mouse
Depositors Institution: The Johns Hopkins University, Department of Biology
Positive Tested Species Reactivity: Avian, Chicken
Antigen Molecular Weight: 47 kDa
Human Protein Atlas:
Predicted Species Reactivity:
Immunogen: crude membrane preparation from leg muscle of 14D chick embryo
Alternate Gene Names:
Alternate Antibody Name:
Alternate Antigen Name:
Myeloma Strain: SP2/0 Ag-14
Epitope Location or Sequence:
Uniprot ID: H6WP41
Entrez Gene ID:
Antibody Registry ID: AB_528094
Additional Information: RRID:AB_528094
Recommended Applications: Function Blocking, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
24 was deposited to the DSHB by Fambrough, D.M. (DSHB Hybridoma Product 24)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
The Journal of biological chemistry
The Journal of biological chemistry 258.6 (1983 Mar 25): 3926-35.