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SKU: 24
View product citations for antibody 24 on CiteAb

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Available: 29

DSHB Data Sheet

Catalog Fields

Antigen: ATPase, (Na(+) K(+)) beta-subunit
Hybridoma Cells Available: Yes
Antigen Species: chicken
Depositor: Fambrough, D.M.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: The Johns Hopkins University, Department of Biology
Positive Tested Species Reactivity: Avian, Chicken
Depositors Notes:
Antigen Molecular Weight: 47 kDa
Human Protein Atlas:
Predicted Species Reactivity:  
Immunogen: crude membrane preparation from leg muscle of 14D chick embryo
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped:
Myeloma Strain: SP2/0 Ag-14
Epitope Location or Sequence:
Uniprot ID: H6WP41 
Immunogen Sequence:
Entrez Gene ID:
Additional Characterization:
Antibody Registry ID: AB_528094 
Additional Information: RRID:AB_528094
Recommended Applications: Function Blocking, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
24 was deposited to the DSHB by Fambrough, D.M. (DSHB Hybridoma Product 24)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

3 References

  • Initial Publication
  • All References
  • Initial Publication

    The Journal of biological chemistry
    Bayne EK
    The Journal of biological chemistry 258.6 (1983 Mar 25): 3926-35.

    All References

    The Journal of biological chemistry
    Bayne EK
    The Journal of biological chemistry 258.6 (1983 Mar 25): 3926-35.

    Cell regulation
    Rizzolo LJ
    Cell regulation 2.11 (1991 Nov): 939-49.

    Journal of cell science
    Li ZQ
    Journal of cell science 106 ( Pt 3). (1993 Nov): 859-67.

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