a5

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SKU: a5

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Supernatant 1.0 ml Concentrate 0.1 ml Frozen Cells 1 vial Growing Cells 1 vial

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DSHB Data Sheet

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Antigen: ATPase, (Na (+) K(+)) alpha subunit

Hybridoma Cells Available: Yes

Antigen Species: Chicken

Depositor: Fambrough, D.M.

Isotype: MIgG1, kappa light chain

Antigen Sequence:

Host Species: mouse

Depositors Institution: Johns Hopkins University

Positive Tested Species Reactivity: Avian, Drosophila, Fish, Frog, Honeybee, Human, Insect, Mackerel, Mammal, Mosquito, Zebrafish

Depositors Notes: Antibody binds a cytosolic epitope on the alpha-subunit of all Na, K-ATPase isoforms. IP recognizes denatured ATPase only.

Antigen Molecular Weight: 112.23 kDa

Human Protein Atlas:

Predicted Species Reactivity:

Gene: ATP1A1; ATPA2; ATP1A3; ATP1A4

Immunogen: IP purified from kidney

Alternate Gene Names: Sodium pump subunit alpha-1

Alternate Antibody Name:

Clonality: Monoclonal

Alternate Antigen Name:

Epitope Mapped: No

Myeloma Strain: SP2/0 Ag8

Epitope Location or Sequence:

Uniprot ID: P09572

Immunogen Sequence: alpha1-beta1 subunits

Entrez Gene ID: 396530

Additional Characterization:

Antibody Registry ID: AB_2166869

Additional Information:

Recommended Applications: FFPE, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot

These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.

For your Materials & Methods section:

a5 was deposited to the DSHB by Fambrough, D.M. (DSHB Hybridoma Product a5)

Storage and Handling Recommendations

Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.

Usage Recommendations

The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

All cell products contain the antimicrobial ProClin. Click here for additional information.

26 References

Initial Publication

IF References

WB References

IHC References

IP References

FFPE References

All References

Initial Publication

Molecular characterization and expression of the (Na+ + K+)-ATPase alpha-subunit in Drosophila melanogaster.
Fambrough DM
The EMBO journal 8.1 (1989 Jan): 193-202.

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a5

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