a5

(0) No Reviews yet
$40.00
SKU: a5
View product citations for antibody a5 on CiteAb

In Stock

Available: 95

DSHB Data Sheet
Back

Catalog Fields

Antigen: ATPase, (Na (+) K(+)) alpha subunit
Hybridoma Cells Available: Yes
Antigen Species: Chicken
Depositor: Fambrough, D.M.
Isotype: MIgG1, kappa light chain
Antigen Sequence:
Host Species: mouse
Depositors Institution: The Johns Hopkins University
Positive Tested Species Reactivity: Avian, Drosophila, Fish, Frog, Honeybee, Human, Insect, Mackerel, Mammal, Mosquito
Depositors Notes: Antibody binds a cytosolic epitope on the alpha-subunit of all Na, K-ATPase isoforms. IP recognizes denatured ATPase only.
Antigen Molecular Weight: 112.23 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: ATP1A1; ATPA2; ATP1A3; ATP1A4
Immunogen: IP purified from kidney
Alternate Gene Names: Sodium pump subunit alpha-1
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: SP2/0 Ag8
Epitope Location or Sequence:
Uniprot ID: P09572 
Immunogen Sequence: alpha1-beta1 subunits
Entrez Gene ID: 396530 
Additional Characterization:  
Antibody Registry ID: AB_2166869 
Additional Information:
Recommended Applications: FFPE, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
See MoreSee Less
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
a5 was deposited to the DSHB by Fambrough, D.M. (DSHB Hybridoma Product a5)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

24 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • IP References
  • FFPE References
  • All References
    • Initial Publication

    Molecular characterization and expression of the (Na+ + K+)-ATPase alpha-subunit in Drosophila melanogaster.
    Fambrough DM
    The EMBO journal 8.1 (1989 Jan): 193-202.

    IF References

    Cdc42 and Rab8a are critical for intestinal stem cell division, survival, and differentiation in mice.
    Gao N
    The Journal of clinical investigation 122.3 (2012 Mar): 1052-65.

    Cdx2 regulates endo-lysosomal function and epithelial cell polarity.
    Kaestner KH
    Genes & development 24.12 (2010 Jun 15): 1295-305.

    P-type Na+/K+-ATPase and V-type H+-ATPase expression patterns in the osmoregulatory organs of larval and adult mosquito Aedes aegypti.
    Gill SS
    The Journal of experimental biology 209.Pt 23 (2006 Dec): 4638-51.

    Energy-dependent regulation of cell structure by AMP-activated protein kinase.
    Chung J
    Nature 447.7147 (2007 Jun 21): 1017-20.

    An animal homolog of plant Mep/Amt transporters promotes ammonia excretion by the anal papillae of the disease vector mosquito Aedes aegypti.
    Donini A
    The Journal of experimental biology 219.Pt 9 (2016 May 1): 1346-55.

    Gene duplications circumvent trade-offs in enzyme function: Insect adaptation to toxic host plants.
    Dobler S
    Evolution; international journal of organic evolution 70.12 (2016 Dec): 2767-2777.

    Secretory cells in honeybee hypopharyngeal gland: polarized organization and age-dependent dynamics of plasma membrane.
    Baumann O
    Cell and tissue research 366.1 (2016 Oct): 163-74.

    Potassium Regulation in Medaka (Oryzias latipes) Larvae Acclimated to Fresh Water: Passive Uptake and Active Secretion by the Skin Cells.
    Lin LY
    Scientific reports 7.1 (2017 Nov 24): 16215.

    Ion uptake pathways in European sea bass Dicentrarchus labrax.
    Lorin-Nebel C
    Gene 692. (2019 Apr 15): 126-137.

    Gene expression profiling of proximal and distal renal tubules in Atlantic salmon (<i>Salmo salar</i>) acclimated to fresh water and seawater.
    Engelund MB
    American journal of physiology. Renal physiology 319.3 (2020 Sep 1): F380-F393.

    Immunohistochemical characterization and change in location of branchial ionocytes after transfer from freshwater to seawater in the euryhaline obscure puffer, Takifugu obscurus.
    Zhao Z
    Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology 190.5 (2020 Sep): 585-596.

    High sucrose diet induces morphological, structural and functional impairments in the renal tubules of Drosophila melanogaster: A model for studying type-2 diabetes mediated renal tubular dysfunction.
    Gautam NK
    Insect biochemistry and molecular biology 125. (2020 Oct): 103441.

    WB References

    Proteomic profiling in Drosophila reveals potential Dube3a regulation of the actin cytoskeleton and neuronal homeostasis.
    Reiter LT
    PloS one 8.4 (2013): e61952.

    Expression profiles of branchial FXYD proteins in the brackish medaka Oryzias dancena: a potential saltwater fish model for studies of osmoregulation.
    Hwang PP
    PloS one 8.1 (2013): e55470.

    Exploration of the mechanisms of protein quality control and osmoregulation in gills of Chromis viridis in response to reduced salinity.
    Tsai SC
    Fish physiology and biochemistry 40.5 (2014 Oct): 1533-46.

    Energy-dependent regulation of cell structure by AMP-activated protein kinase.
    Chung J
    Nature 447.7147 (2007 Jun 21): 1017-20.

    Immunohistochemical characterization and change in location of branchial ionocytes after transfer from freshwater to seawater in the euryhaline obscure puffer, Takifugu obscurus.
    Zhao Z
    Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology 190.5 (2020 Sep): 585-596.

    Immunological characterization of two types of ionocytes in the inner ear epithelium of Pacific Chub Mackerel (Scomber japonicus).
    Tresguerres M
    Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology 190.4 (2020 Jul): 419-431.

    Exposure to pH 3.5 water has no effect on the gills of the Amazonian tambaqui (Colossoma macropomum).
    Val AL
    Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology 191.3 (2021 May): 493-502.

    Changes in gill and air-breathing organ characteristics during the transition from water- to air-breathing in juvenile Arapaima gigas.
    Brauner CJ
    Journal of experimental zoology. Part A, Ecological and integrative physiology . (2021 Apr 5): .

    IHC References

    Expression profiles of branchial FXYD proteins in the brackish medaka Oryzias dancena: a potential saltwater fish model for studies of osmoregulation.
    Hwang PP
    PloS one 8.1 (2013): e55470.

    P-type Na+/K+-ATPase and V-type H+-ATPase expression patterns in the osmoregulatory organs of larval and adult mosquito Aedes aegypti.
    Gill SS
    The Journal of experimental biology 209.Pt 23 (2006 Dec): 4638-51.

    Axon fasciculation defects and retinal dysplasias in mice lacking the immunoglobulin superfamily adhesion molecule BEN/ALCAM/SC1.
    Sanes JR
    Molecular and cellular neurosciences 27.1 (2004 Sep): 59-69.

    Drosophila Src regulates anisotropic apical surface growth to control epithelial tube size.
    Beitel GJ
    Nature cell biology 14.5 (2012 Mar 25): 518-25.

    Immunological characterization of two types of ionocytes in the inner ear epithelium of Pacific Chub Mackerel (Scomber japonicus).
    Tresguerres M
    Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology 190.4 (2020 Jul): 419-431.

    Exposure to pH 3.5 water has no effect on the gills of the Amazonian tambaqui (Colossoma macropomum).
    Val AL
    Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology 191.3 (2021 May): 493-502.

    Membrane potential regulates Hedgehog signalling in the Drosophila wing imaginal disc.
    Hariharan IK
    EMBO reports 22.4 (2021 Apr 7): e51861.

    Changes in gill and air-breathing organ characteristics during the transition from water- to air-breathing in juvenile Arapaima gigas.
    Brauner CJ
    Journal of experimental zoology. Part A, Ecological and integrative physiology . (2021 Apr 5): .

    IP References

    Expression profiles of branchial FXYD proteins in the brackish medaka Oryzias dancena: a potential saltwater fish model for studies of osmoregulation.
    Hwang PP
    PloS one 8.1 (2013): e55470.

    FFPE References

    An animal homolog of plant Mep/Amt transporters promotes ammonia excretion by the anal papillae of the disease vector mosquito Aedes aegypti.
    Donini A
    The Journal of experimental biology 219.Pt 9 (2016 May 1): 1346-55.

    Exposure to pH 3.5 water has no effect on the gills of the Amazonian tambaqui (Colossoma macropomum).
    Val AL
    Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology 191.3 (2021 May): 493-502.

    All References

    Expression profiles of branchial FXYD proteins in the brackish medaka Oryzias dancena: a potential saltwater fish model for studies of osmoregulation.
    Hwang PP
    PloS one 8.1 (2013): e55470.

    P-type Na+/K+-ATPase and V-type H+-ATPase expression patterns in the osmoregulatory organs of larval and adult mosquito Aedes aegypti.
    Gill SS
    The Journal of experimental biology 209.Pt 23 (2006 Dec): 4638-51.

    Axon fasciculation defects and retinal dysplasias in mice lacking the immunoglobulin superfamily adhesion molecule BEN/ALCAM/SC1.
    Sanes JR
    Molecular and cellular neurosciences 27.1 (2004 Sep): 59-69.

    Drosophila Src regulates anisotropic apical surface growth to control epithelial tube size.
    Beitel GJ
    Nature cell biology 14.5 (2012 Mar 25): 518-25.

    Immunological characterization of two types of ionocytes in the inner ear epithelium of Pacific Chub Mackerel (Scomber japonicus).
    Tresguerres M
    Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology 190.4 (2020 Jul): 419-431.

    Exposure to pH 3.5 water has no effect on the gills of the Amazonian tambaqui (Colossoma macropomum).
    Val AL
    Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology 191.3 (2021 May): 493-502.

    Membrane potential regulates Hedgehog signalling in the Drosophila wing imaginal disc.
    Hariharan IK
    EMBO reports 22.4 (2021 Apr 7): e51861.

    Changes in gill and air-breathing organ characteristics during the transition from water- to air-breathing in juvenile Arapaima gigas.
    Brauner CJ
    Journal of experimental zoology. Part A, Ecological and integrative physiology . (2021 Apr 5): .

    Cdc42 and Rab8a are critical for intestinal stem cell division, survival, and differentiation in mice.
    Gao N
    The Journal of clinical investigation 122.3 (2012 Mar): 1052-65.

    Cdx2 regulates endo-lysosomal function and epithelial cell polarity.
    Kaestner KH
    Genes & development 24.12 (2010 Jun 15): 1295-305.

    Energy-dependent regulation of cell structure by AMP-activated protein kinase.
    Chung J
    Nature 447.7147 (2007 Jun 21): 1017-20.

    An animal homolog of plant Mep/Amt transporters promotes ammonia excretion by the anal papillae of the disease vector mosquito Aedes aegypti.
    Donini A
    The Journal of experimental biology 219.Pt 9 (2016 May 1): 1346-55.

    Gene duplications circumvent trade-offs in enzyme function: Insect adaptation to toxic host plants.
    Dobler S
    Evolution; international journal of organic evolution 70.12 (2016 Dec): 2767-2777.

    Secretory cells in honeybee hypopharyngeal gland: polarized organization and age-dependent dynamics of plasma membrane.
    Baumann O
    Cell and tissue research 366.1 (2016 Oct): 163-74.

    Potassium Regulation in Medaka (Oryzias latipes) Larvae Acclimated to Fresh Water: Passive Uptake and Active Secretion by the Skin Cells.
    Lin LY
    Scientific reports 7.1 (2017 Nov 24): 16215.

    Ion uptake pathways in European sea bass Dicentrarchus labrax.
    Lorin-Nebel C
    Gene 692. (2019 Apr 15): 126-137.

    Gene expression profiling of proximal and distal renal tubules in Atlantic salmon (<i>Salmo salar</i>) acclimated to fresh water and seawater.
    Engelund MB
    American journal of physiology. Renal physiology 319.3 (2020 Sep 1): F380-F393.

    Immunohistochemical characterization and change in location of branchial ionocytes after transfer from freshwater to seawater in the euryhaline obscure puffer, Takifugu obscurus.
    Zhao Z
    Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology 190.5 (2020 Sep): 585-596.

    High sucrose diet induces morphological, structural and functional impairments in the renal tubules of Drosophila melanogaster: A model for studying type-2 diabetes mediated renal tubular dysfunction.
    Gautam NK
    Insect biochemistry and molecular biology 125. (2020 Oct): 103441.

    Proteomic profiling in Drosophila reveals potential Dube3a regulation of the actin cytoskeleton and neuronal homeostasis.
    Reiter LT
    PloS one 8.4 (2013): e61952.

    Exploration of the mechanisms of protein quality control and osmoregulation in gills of Chromis viridis in response to reduced salinity.
    Tsai SC
    Fish physiology and biochemistry 40.5 (2014 Oct): 1533-46.

    Molecular characterization and expression of the (Na+ + K+)-ATPase alpha-subunit in Drosophila melanogaster.
    Fambrough DM
    The EMBO journal 8.1 (1989 Jan): 193-202.

    Cloning and characterization of AgCA9, a novel alpha-carbonic anhydrase from Anopheles gambiae Giles sensu stricto (Diptera: Culicidae) larvae.
    Linser PJ
    The Journal of experimental biology 210.Pt 22 (2007 Nov): 3919-30.

    Slc4-like anion transporters of the larval mosquito alimentary canal.
    Romero MF
    Journal of insect physiology 58.4 (2012 Apr): 551-62.

    Back

    Ratings & Reviews

    No reviews available

    Be the first to Write a Review