a5
Catalog Fields
Clone ID/Product Name: a5
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Symbol: ATP1A1; ATPA2; ATP1A3; ATP1A4
Ab Isotype: MIgG1, kappa light chain
Gene Name:
Antibody Registry ID: AB_2166869
Uniprot ID: P09572
Entrez Gene ID: 396530
Clonality: Monoclonal
Immunogen: IP purified from kidney
Clone:
Immunogen Sequence: alpha1-beta1 subunits
Myeloma Strain: SP2/0 Ag8
Epitope Mapped: No
Antigen Name: ATPase, (Na (+) K(+)) alpha subunit
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 10/10/1990
Antigen Molecular Weight: 112.23 kDa
Depositor: Fambrough, D.M.
Antigen Sequence:
Depositor Institution: Johns Hopkins University
Antigen Species: Chicken
Depositor Notes: Antibody binds a cytosolic epitope on the alpha-subunit of all Na, K-ATPase isoforms. IP recognizes denatured ATPase only.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Avian, Drosophila, Fish, Frog, Honeybee, Human, Insect, Mackerel, Mammal, Mosquito, Zebrafish
Additional Information:
Recommended Applications: FFPE, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
a5 was deposited to the DSHB by Fambrough, D.M. (DSHB Hybridoma Product a5)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
26 References
Initial Publication