a5
Catalog Fields
Antigen: ATPase, (Na (+) K(+)) alpha subunit
Hybridoma Cells Available: Yes
Antigen Species: Chicken
Depositor: Fambrough, D.M.
Isotype: MIgG1, kappa light chain
Depositors Institution: The Johns Hopkins University
Host Species: mouse
Depositors Notes: Antibody binds a cytosolic epitope on the alpha-subunit of all Na, K-ATPase isoforms. IP recognizes denatured ATPase only.
Positive Tested Species Reactivity: Avian, Fish, Frog, Honeybee, Human, Insect, Mammal, Mosquito
Gene: ATP1A1; ATPA2; ATP1A3; ATP1A4
Antigen Molecular Weight: 112.23 kDa
Alternate Gene Names: Sodium pump subunit alpha-1
Immunogen: IP purified from kidney
Clonality: Monoclonal
Myeloma Strain: SP2/0 Ag8
Immunogen Sequence: alpha1-beta1 subunits
Uniprot ID: P09572
Entrez Gene ID: 396530
Antibody Registry ID: AB_2166869
Recommended Applications: FFPE, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
a5 was deposited to the DSHB by Fambrough, D.M. (DSHB Hybridoma Product a5)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.
17 References
Initial Publication
Molecular characterization and expression of the (Na+ + K+)-ATPase alpha-subunit in Drosophila melanogaster.
Fambrough DM
The EMBO journal 8.1 (1989 Jan): 193-202.