2B49
Catalog Fields
Antigen: phosphacan/protein tyrosine phosphatase - z/b
Available to For-Profits: Yes
Antigen Species: rat
Hybridoma Cells Available (Non-Profit): Yes
Isotype: MIgG1
Depositor: Margolis, R.K. / Margolis, R.U.
Host Species: mouse
Antigen Sequence:
Positive Tested Species Reactivity: Rat
Depositors Institution: New York University Medical Center / State University of New York
Antigen Molecular Weight:
Depositors Notes: For immunocytochemistry use ascites at dilutions of 1:100-1:500. However, frequently grows as solid tumors, so yield of ascites may be very small. Hybridoma supernatant is most economical for use with immunoblots.
Immunogen: PBS-soluble chondroitin sulfate proteoglycans from rat brain
Gene: Ptprz1
Alternate Antibody Name: protein tyrosine phosphatase - z/b
Alternate Gene Names:
Alternate Antigen Name:
Clonality: Monoclonal
Myeloma Strain: PAI
Epitope Mapped: No
Uniprot ID: Q62656
Epitope Location or Sequence:
Entrez Gene ID: 25613
Immunogen Sequence:
Antibody Registry ID: AB_2173126
Recommended Applications: Immunohistochemistry, Western Blot
Additional Information: Protein tyrosine phosphatase that negatively regulates oligodendrocyte precursor proliferation in the embryonic spinal cord. Required for normal differentiation of the precursor cells into mature, fully myelinating oligodendrocytes. May play a role in protecting oligondendrocytes against apoptosis. May play a role in the establishment of contextual memory, probably via the dephosphorylation of proteins that are part of important signaling cascades RRID:AB_2173126
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
2B49 was deposited to the DSHB by Margolis, R.K. / Margolis, R.U. (DSHB Hybridoma Product 2B49)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
3 References
Initial Publication
Phosphacan, a chondroitin sulfate proteoglycan of brain that interacts with neurons and neural cell-adhesion molecules, is an extracellular variant of a receptor-type protein tyrosine phosphatase.
Margolis RU
Proceedings of the National Academy of Sciences of the United States of America 91.7 (1994 Mar 29): 2512-6.