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$45.00 to $425.00
SKU: 2B49
View product citations for antibody 2B49 on CiteAb

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Available: 17

DSHB Data Sheet

Catalog Fields

Antigen: phosphacan/protein tyrosine phosphatase - z/b
Hybridoma Cells Available: Yes
Antigen Species: rat
Depositor: Margolis, R.K. / Margolis, R.U.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: New York University Medical Center / State University of New York
Positive Tested Species Reactivity: Rat
Depositors Notes: For immunocytochemistry use ascites at dilutions of 1:100-1:500. However, frequently grows as solid tumors, so yield of ascites may be very small. Hybridoma supernatant is most economical for use with immunoblots.
Antigen Molecular Weight:
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: Ptprz1
Immunogen: PBS-soluble chondroitin sulfate proteoglycans from rat brain
Alternate Gene Names:
Alternate Antibody Name: protein tyrosine phosphatase - z/b
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: PAI
Epitope Location or Sequence:
Uniprot ID: Q62656 
Immunogen Sequence:
Entrez Gene ID: 25613 
Additional Characterization:  
Antibody Registry ID: AB_2173126 
Additional Information: Protein tyrosine phosphatase that negatively regulates oligodendrocyte precursor proliferation in the embryonic spinal cord. Required for normal differentiation of the precursor cells into mature, fully myelinating oligodendrocytes. May play a role in protecting oligondendrocytes against apoptosis. May play a role in the establishment of contextual memory, probably via the dephosphorylation of proteins that are part of important signaling cascades RRID:AB_2173126
Recommended Applications: Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
2B49 was deposited to the DSHB by Margolis, R.K. / Margolis, R.U. (DSHB Hybridoma Product 2B49)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
All cell products contain the antimicrobial ProClin. Click here for additional information.

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