2B49

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$40.00
SKU: 2B49
View product citations for antibody 2B49 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: phosphacan/protein tyrosine phosphatase - z/b
Hybridoma Cells Available: Yes
Antigen Species: rat
Depositor: Margolis, R.K. / Margolis, R.U.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: New York University Medical Center, Department of Pharmacology
Positive Tested Species Reactivity: Rat
Depositors Notes: For immunocytochemistry use ascites at dilutions of 1:100-1:500. However, frequently grows as solid tumors, so yield of ascites may be very small. Hybridoma supernatant is most economical for use with immunoblots.
Antigen Molecular Weight:
Human Protein Atlas:
Predicted Species Reactivity:  
Gene: Ptprz1
Immunogen: PBS-soluble chondroitin sulfate proteoglycans from rat brain
Alternate Gene Names:
Alternate Antibody Name: protein tyrosine phosphatase - z/b
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped:
Myeloma Strain: PAI
Epitope Location or Sequence:
Uniprot ID: Q62656 
Immunogen Sequence:
Entrez Gene ID: 25613 
Additional Characterization:
Antibody Registry ID: AB_2173126 
Additional Information: Protein tyrosine phosphatase that negatively regulates oligodendrocyte precursor proliferation in the embryonic spinal cord. Required for normal differentiation of the precursor cells into mature, fully myelinating oligodendrocytes. May play a role in protecting oligondendrocytes against apoptosis. May play a role in the establishment of contextual memory, probably via the dephosphorylation of proteins that are part of important signaling cascades RRID:AB_2173126
Recommended Applications: Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
2B49 was deposited to the DSHB by Margolis, R.K. / Margolis, R.U. (DSHB Hybridoma Product 2B49)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

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