12C5

(0) No Reviews yet
$40.00
SKU: 12C5
View product citations for antibody 12C5 on CiteAb

In Stock

Available: 97

DSHB Data Sheet

Catalog Fields

Antigen: versican (hyaluronate-binding region)
Hybridoma Cells Available:
Antigen Species: Human
Depositor: Asher, R.A.
Isotype: MIgG1
Antigen Sequence: Conserved sequence in N-terminal G1 domain
Host Species: mouse
Depositors Institution: Cambridge Centre for Brain Repair
Positive Tested Species Reactivity: Bovine, Canine, Human, Porcine, Rat
Depositors Notes: Blot: yes, but sensitive to reduction
Antigen Molecular Weight: Apparent: >250kD
Human Protein Atlas:
Predicted Species Reactivity:  
Gene: VCAN
Immunogen: hyaluronate-binding region of versican (aka _GHAP_)
Alternate Gene Names: WGN; ERVR; GHAP; PG-M; WGN1; CSPG2
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: NS-1
Epitope Location or Sequence: G1 domain
Uniprot ID: P13611 
Immunogen Sequence: same as antigen sequence.
Entrez Gene ID: 1462 
Additional Characterization:
Antibody Registry ID: AB_528503 
Additional Information:
Recommended Applications: ELISA, FFPE, Function Blocking, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Microarray, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
12C5 was deposited to the DSHB by Asher, R.A. (DSHB Hybridoma Product 12C5)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

31 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • IP References
  • ELISA References
  • FFPE References
  • FB References
  • Microarray References
  • Epitope Map References
  • All References
  • Initial Publication
    IF References

    Elastic fiber-mediated enthesis in the human middle ear.
    Fujimiya M
    Journal of anatomy 221.4 (2012 Oct): 331-40.

    Differential distribution of myosin isoforms among the myofibrils of individual developing muscle fibers.
    Gauthier GF
    The Journal of cell biology 110.3 (1990 Mar): 693-701.

    Mapping the differential distribution of proteoglycan core proteins in the adult human retina, choroid, and sclera.
    Bishop PN
    Investigative ophthalmology and visual science 53.12 (2012 Nov 7): 7528-38.

    Isolation of a large aggregating proteoglycan from human brain.
    Bignami A
    The Journal of biological chemistry 267.33 (1992 Nov 25): 23883-7.

    Changes in distribution, cell associations, and protein expression levels of NG2, neurocan, phosphacan, brevican, versican V2, and tenascin-C during acute to chronic maturation of spinal cord scar tissue.
    Davies SJ
    Journal of neuroscience research 71.3 (2003 Feb 1): 427-44.

    Versican is upregulated in CNS injury and is a product of oligodendrocyte lineage cells.
    Fawcett JW
    The Journal of neuroscience : the official journal of the Society for Neuroscience 22.6 (2002 Mar 15): 2225-36.

    Versican regulates metastasis of epithelial ovarian carcinoma cells and spheroids.
    Barbolina MV
    Journal of ovarian research 7. (2014): 70.

    On the existence of a cartilage-like proteoglycan and link proteins in the central nervous system.
    Bignami A
    Glia 13.4 (1995 Apr): 294-308.

    WB References

    Extracellular matrix of central nervous system white matter: demonstration of an hyaluronate-protein complex.
    Bignami A
    Journal of neuroscience research 28.3 (1991 Mar): 410-21.

    Versican is upregulated in CNS injury and is a product of oligodendrocyte lineage cells.
    Fawcett JW
    The Journal of neuroscience : the official journal of the Society for Neuroscience 22.6 (2002 Mar 15): 2225-36.

    Changes in distribution, cell associations, and protein expression levels of NG2, neurocan, phosphacan, brevican, versican V2, and tenascin-C during acute to chronic maturation of spinal cord scar tissue.
    Davies SJ
    Journal of neuroscience research 71.3 (2003 Feb 1): 427-44.

    Distribution of PG-M/versican variants in human tissues and de novo expression of isoform V3 upon endothelial cell activation, migration, and neoangiogenesis in vitro.
    Perris R
    The Journal of biological chemistry 277.49 (2002 Dec 6): 47626-35.

    Comparing astrocytic cell lines that are inhibitory or permissive for axon growth: the major axon-inhibitory proteoglycan is NG2.
    Fawcett JW
    The Journal of neuroscience : the official journal of the Society for Neuroscience 19.20 (1999 Oct 15): 8778-88.

    Age-related differences in human skin proteoglycans.
    Caplan AI
    Glycobiology 21.2 (2011 Feb): 257-68.

    Polyinosine-polycytidylic acid stimulates versican accumulation in the extracellular matrix promoting monocyte adhesion.
    Wight TN
    American journal of respiratory cell and molecular biology 43.1 (2010 Jul): 109-20.

    RhoGDI2 suppresses lung metastasis in mice by reducing tumor versican expression and macrophage infiltration.
    Theodorescu D
    The Journal of clinical investigation 122.4 (2012 Apr): 1503-18.

    Versican regulates metastasis of epithelial ovarian carcinoma cells and spheroids.
    Barbolina MV
    Journal of ovarian research 7. (2014): 70.

    On the existence of a cartilage-like proteoglycan and link proteins in the central nervous system.
    Bignami A
    Glia 13.4 (1995 Apr): 294-308.

    IHC References

    Extracellular matrix of central nervous system white matter: demonstration of an hyaluronate-protein complex.
    Bignami A
    Journal of neuroscience research 28.3 (1991 Mar): 410-21.

    Versican is upregulated in CNS injury and is a product of oligodendrocyte lineage cells.
    Fawcett JW
    The Journal of neuroscience : the official journal of the Society for Neuroscience 22.6 (2002 Mar 15): 2225-36.

    Changes in distribution, cell associations, and protein expression levels of NG2, neurocan, phosphacan, brevican, versican V2, and tenascin-C during acute to chronic maturation of spinal cord scar tissue.
    Davies SJ
    Journal of neuroscience research 71.3 (2003 Feb 1): 427-44.

    Preparative high-performance liquid chromatography and preparative thin-layer chromatography isolation of tocainide carbamoyl-O-beta-D-glucuronide: structural characterization by gas chromatography-mass spectrometry and fast atom bombardment-mass spectrometry.
    McErlane KM
    Journal of pharmaceutical sciences 79.10 (1990 Oct): 857-61.

    Distribution of PG-M/versican variants in human tissues and de novo expression of isoform V3 upon endothelial cell activation, migration, and neoangiogenesis in vitro.
    Perris R
    The Journal of biological chemistry 277.49 (2002 Dec 6): 47626-35.

    Erythropoietin-mediated preservation of the white matter in rat spinal cord injury.
    De Biasi S
    Neuroscience 144.3 (2007 Feb 9): 865-77.

    Comparing astrocytic cell lines that are inhibitory or permissive for axon growth: the major axon-inhibitory proteoglycan is NG2.
    Fawcett JW
    The Journal of neuroscience : the official journal of the Society for Neuroscience 19.20 (1999 Oct 15): 8778-88.

    Polyinosine-polycytidylic acid stimulates versican accumulation in the extracellular matrix promoting monocyte adhesion.
    Wight TN
    American journal of respiratory cell and molecular biology 43.1 (2010 Jul): 109-20.

    Identification of stromal proteins overexpressed in nodular sclerosis Hodgkin lymphoma.
    Castronovo V
    Proteome science 9.1 (2011 Oct 5): 63.

    Localization and functional characterization of glycosaminoglycan domains in the normal human kidney as revealed by phage display-derived single chain antibodies.
    van Kuppevelt TH
    Journal of the American Society of Nephrology : JASN 16.5 (2005 May): 1279-88.

    Elastic fiber-mediated enthesis in the human middle ear.
    Fujimiya M
    Journal of anatomy 221.4 (2012 Oct): 331-40.

    An immunohistochemical study of the dorsal capsule of the lumbar and thoracic facet joints.
    Milz S
    Spine 26.15 (2001 Aug 1): E338-43.

    Fibrocartilage in the transverse ligament of the human atlas.
    Benjamin M
    Spine 26.16 (2001 Aug 15): 1765-71.

    Fibrocartilage in the transverse ligament of the human acetabulum.
    Benjamin M
    Journal of anatomy 198.Pt 2 (2001 Feb): 223-8.

    Existence of reserve quiescent stem cells in adults, from amphibians to humans.
    Young HE
    Current topics in microbiology and immunology 280. (2004): 71-109.

    IP References

    Glial hyaluronate-binding protein: a product of metalloproteinase digestion of versican?
    Bignami A
    The Biochemical journal 312 ( Pt 2). (1995 Dec 1): 377-84.

    Comparing astrocytic cell lines that are inhibitory or permissive for axon growth: the major axon-inhibitory proteoglycan is NG2.
    Fawcett JW
    The Journal of neuroscience : the official journal of the Society for Neuroscience 19.20 (1999 Oct 15): 8778-88.

    ELISA References

    Comparing astrocytic cell lines that are inhibitory or permissive for axon growth: the major axon-inhibitory proteoglycan is NG2.
    Fawcett JW
    The Journal of neuroscience : the official journal of the Society for Neuroscience 19.20 (1999 Oct 15): 8778-88.

    The large chondroitin sulphate proteoglycan versican in mammalian vitreous.
    Bishop PN
    Matrix biology : journal of the International Society for Matrix Biology 17.5 (1998 Oct): 325-33.

    Experimental immunity to the G1 domain of the proteoglycan versican induces spondylitis and sacroiliitis, of a kind seen in human spondylarthropathies.
    Zhang Y
    Arthritis and rheumatism 48.10 (2003 Oct): 2903-15.

    FFPE References

    Identification of stromal proteins overexpressed in nodular sclerosis Hodgkin lymphoma.
    Castronovo V
    Proteome science 9.1 (2011 Oct 5): 63.

    Aggrecan, versican and type VI collagen are components of annular translamellar crossbridges in the intervertebral disc.
    Little CB
    European spine journal : official publication of the European Spine Society, the European Spinal Deformity Society, and the European Section of the Cervical Spine Research Society 17.2 (2008 Feb): 314-24.

    An immunohistochemical study of the rabbit suprapatella, a sesamoid fibrocartilage in the quadriceps tendon containing aggrecan.
    Benjamin M
    The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 50.7 (2002 Jul): 955-60.

    Experimental immunity to the G1 domain of the proteoglycan versican induces spondylitis and sacroiliitis, of a kind seen in human spondylarthropathies.
    Zhang Y
    Arthritis and rheumatism 48.10 (2003 Oct): 2903-15.

    Versican expression in myoepithelial cells from carcinomas in canine mixed mammary tumors.
    Cassali GD
    Veterinary journal (London, England : 1997) 200.1 (2014 Apr): 146-51.

    FB References

    Versican regulates metastasis of epithelial ovarian carcinoma cells and spheroids.
    Barbolina MV
    Journal of ovarian research 7. (2014): 70.

    Microarray References
    Epitope Map References
    All References

    Extracellular matrix of central nervous system white matter: demonstration of an hyaluronate-protein complex.
    Bignami A
    Journal of neuroscience research 28.3 (1991 Mar): 410-21.

    Versican is upregulated in CNS injury and is a product of oligodendrocyte lineage cells.
    Fawcett JW
    The Journal of neuroscience : the official journal of the Society for Neuroscience 22.6 (2002 Mar 15): 2225-36.

    Changes in distribution, cell associations, and protein expression levels of NG2, neurocan, phosphacan, brevican, versican V2, and tenascin-C during acute to chronic maturation of spinal cord scar tissue.
    Davies SJ
    Journal of neuroscience research 71.3 (2003 Feb 1): 427-44.

    Preparative high-performance liquid chromatography and preparative thin-layer chromatography isolation of tocainide carbamoyl-O-beta-D-glucuronide: structural characterization by gas chromatography-mass spectrometry and fast atom bombardment-mass spectrometry.
    McErlane KM
    Journal of pharmaceutical sciences 79.10 (1990 Oct): 857-61.

    Distribution of PG-M/versican variants in human tissues and de novo expression of isoform V3 upon endothelial cell activation, migration, and neoangiogenesis in vitro.
    Perris R
    The Journal of biological chemistry 277.49 (2002 Dec 6): 47626-35.

    Erythropoietin-mediated preservation of the white matter in rat spinal cord injury.
    De Biasi S
    Neuroscience 144.3 (2007 Feb 9): 865-77.

    Comparing astrocytic cell lines that are inhibitory or permissive for axon growth: the major axon-inhibitory proteoglycan is NG2.
    Fawcett JW
    The Journal of neuroscience : the official journal of the Society for Neuroscience 19.20 (1999 Oct 15): 8778-88.

    Polyinosine-polycytidylic acid stimulates versican accumulation in the extracellular matrix promoting monocyte adhesion.
    Wight TN
    American journal of respiratory cell and molecular biology 43.1 (2010 Jul): 109-20.

    Identification of stromal proteins overexpressed in nodular sclerosis Hodgkin lymphoma.
    Castronovo V
    Proteome science 9.1 (2011 Oct 5): 63.

    Localization and functional characterization of glycosaminoglycan domains in the normal human kidney as revealed by phage display-derived single chain antibodies.
    van Kuppevelt TH
    Journal of the American Society of Nephrology : JASN 16.5 (2005 May): 1279-88.

    Elastic fiber-mediated enthesis in the human middle ear.
    Fujimiya M
    Journal of anatomy 221.4 (2012 Oct): 331-40.

    An immunohistochemical study of the dorsal capsule of the lumbar and thoracic facet joints.
    Milz S
    Spine 26.15 (2001 Aug 1): E338-43.

    Fibrocartilage in the transverse ligament of the human atlas.
    Benjamin M
    Spine 26.16 (2001 Aug 15): 1765-71.

    Fibrocartilage in the transverse ligament of the human acetabulum.
    Benjamin M
    Journal of anatomy 198.Pt 2 (2001 Feb): 223-8.

    Existence of reserve quiescent stem cells in adults, from amphibians to humans.
    Young HE
    Current topics in microbiology and immunology 280. (2004): 71-109.

    Differential distribution of myosin isoforms among the myofibrils of individual developing muscle fibers.
    Gauthier GF
    The Journal of cell biology 110.3 (1990 Mar): 693-701.

    Mapping the differential distribution of proteoglycan core proteins in the adult human retina, choroid, and sclera.
    Bishop PN
    Investigative ophthalmology and visual science 53.12 (2012 Nov 7): 7528-38.

    Isolation of a large aggregating proteoglycan from human brain.
    Bignami A
    The Journal of biological chemistry 267.33 (1992 Nov 25): 23883-7.

    Versican regulates metastasis of epithelial ovarian carcinoma cells and spheroids.
    Barbolina MV
    Journal of ovarian research 7. (2014): 70.

    On the existence of a cartilage-like proteoglycan and link proteins in the central nervous system.
    Bignami A
    Glia 13.4 (1995 Apr): 294-308.

    Age-related differences in human skin proteoglycans.
    Caplan AI
    Glycobiology 21.2 (2011 Feb): 257-68.

    RhoGDI2 suppresses lung metastasis in mice by reducing tumor versican expression and macrophage infiltration.
    Theodorescu D
    The Journal of clinical investigation 122.4 (2012 Apr): 1503-18.

    Glial hyaluronate-binding protein: a product of metalloproteinase digestion of versican?
    Bignami A
    The Biochemical journal 312 ( Pt 2). (1995 Dec 1): 377-84.

    The large chondroitin sulphate proteoglycan versican in mammalian vitreous.
    Bishop PN
    Matrix biology : journal of the International Society for Matrix Biology 17.5 (1998 Oct): 325-33.

    Experimental immunity to the G1 domain of the proteoglycan versican induces spondylitis and sacroiliitis, of a kind seen in human spondylarthropathies.
    Zhang Y
    Arthritis and rheumatism 48.10 (2003 Oct): 2903-15.

    Aggrecan, versican and type VI collagen are components of annular translamellar crossbridges in the intervertebral disc.
    Little CB
    European spine journal : official publication of the European Spine Society, the European Spinal Deformity Society, and the European Section of the Cervical Spine Research Society 17.2 (2008 Feb): 314-24.

    An immunohistochemical study of the rabbit suprapatella, a sesamoid fibrocartilage in the quadriceps tendon containing aggrecan.
    Benjamin M
    The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 50.7 (2002 Jul): 955-60.

    Versican expression in myoepithelial cells from carcinomas in canine mixed mammary tumors.
    Cassali GD
    Veterinary journal (London, England : 1997) 200.1 (2014 Apr): 146-51.

    Identification of specific reachable molecular targets in human breast cancer using a versatile ex vivo proteomic method.
    Waltregny D
    Proteomics 7.8 (2007 Apr): 1188-96.

    Multiple domains of the large fibroblast proteoglycan, versican.
    Ruoslahti E
    The EMBO journal 8.10 (1989 Oct): 2975-81.

    Drosophila Hindsight and mammalian RREB-1 are evolutionarily conserved DNA-binding transcriptional attenuators.
    Lipshitz HD
    Differentiation; research in biological diversity 86.4-5 (2013 Nov-Dec): 159-70.

    Ratings & Reviews

    No reviews available

    Be the first to Write a Review