2B9

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$40.00
SKU: 2B9
View product citations for antibody 2B9 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: collagen type IX
Hybridoma Cells Available: Yes
Antigen Species: chicken
Depositor: Halfter, W.M.
Isotype: MIgM
Antigen Sequence:
Host Species: mouse
Depositors Institution: Department of Neurobiology, University of Pittsburgh
Positive Tested Species Reactivity: Chicken, Quail
Depositors Notes:
Antigen Molecular Weight: Apparent : 115 kDa, and doublet at 97 kDa, 93 kDa
Human Protein Atlas:
Predicted Species Reactivity:  
Gene:
Immunogen: chick vitreous body
Alternate Gene Names:
Alternate Antibody Name:
Clonality:
Alternate Antigen Name:
Epitope Mapped:
Myeloma Strain: Ag8
Epitope Location or Sequence:
Uniprot ID:
Immunogen Sequence:
Entrez Gene ID:
Additional Characterization:
Antibody Registry ID:
Additional Information:
Recommended Applications: Immunofluorescence, Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
2B9 was deposited to the DSHB by Halfter, W.M. (DSHB Hybridoma Product 2B9)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

2 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • All References
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