II-II6B3

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$40.00
SKU: II-II6B3
View product citations for antibody II-II6B3 on CiteAb

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Available: 170

DSHB Data Sheet

Catalog Fields

Antigen: Collagen type II
Hybridoma Cells Available: Yes
Antigen Species: Chicken
Depositor: Linsenmayer, T.F.
Isotype: MIgG1, kappa light chain
Antigen Sequence:
Host Species: mouse
Depositors Institution: Tufts Medical School
Positive Tested Species Reactivity: Avian, Bovine, Broad species, Chicken, Fish, Goat, Human, Mouse, Ovine, Rabbit, Rat, Shark, Xenopus, Zebrafish
Depositors Notes:
Antigen Molecular Weight: 141 kDa (predicted)
Human Protein Atlas:
Predicted Species Reactivity:  
Gene: COL2A1
Immunogen: Pepsin-digest adult chick sternal cartilage
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: MPC 11
Epitope Location or Sequence: Epitope lies within the triple-helical domain of chick collagen type II.
Uniprot ID: P02460 
Immunogen Sequence: Full length protein
Entrez Gene ID: 395069 
Additional Characterization:
Antibody Registry ID: AB_528165 
Additional Information:
Recommended Applications: FFPE, Immunofluorescence, Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
II-II6B3 was deposited to the DSHB by Linsenmayer, T.F. (DSHB Hybridoma Product II-II6B3)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

47 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • FFPE References
  • Epitope Map References
  • All References
  • Initial Publication

    Monoclonal antibodies to connective tissue macromolecules: type II collagen.
    Hendrix MJ
    Biochemical and biophysical research communications 92.2 (1980 Jan 29): 440-6.

    IF References
    WB References

    Reconstruction of an in vitro tissue-specific microenvironment to rejuvenate synovium-derived stem cells for cartilage tissue engineering.
    Pei M
    Tissue engineering. Part A 15.12 (2009 Dec): 3809-21.

    Collagen of accessory structures of organ of Corti.
    Thalmann I
    Connective tissue research 29.3 (1993): 191-201.

    Engineering of functional cartilage tissue using stem cells from synovial lining: a preliminary study.
    Vunjak-Novakovic G
    Clinical orthopaedics and related research 466.8 (2008 Aug): 1880-9.

    IHC References

    Distinct patterns of notochord mineralization in zebrafish coincide with the localization of Osteocalcin isoform 1 during early vertebral centra formation.
    Witten PE
    BMC developmental biology 12. (2012 Oct 9): 28.

    Ret signalling integrates a craniofacial muscle module during development.
    Roehl H
    Development (Cambridge, England) 138.10 (2011 May): 2015-24.

    Collagen IX is required for the integrity of collagen II fibrils and the regulation of vascular plexus formation in zebrafish caudal fins.
    Yu J
    Developmental biology 332.2 (2009 Aug 15): 360-70.

    Regulation of zebrafish skeletogenesis by ext2/dackel and papst1/pinscher.
    Roehl HH
    PLoS genetics 4.7 (2008 Jul 25): e1000136.

    Hypoxic culture of bone marrow-derived mesenchymal stromal stem cells differentially enhances in vitro chondrogenesis within cell-seeded collagen and hyaluronic acid porous scaffolds.
    Adesida AB
    Stem cell research and therapy 6. (2015 Apr 23): 84.

    Matrix formation is enhanced in co-cultures of human meniscus cells with bone marrow stromal cells.
    Adesida AB
    Journal of tissue engineering and regenerative medicine 7.12 (2013 Dec): 965-73.

    Matrix forming characteristics of inner and outer human meniscus cells on 3D collagen scaffolds under normal and low oxygen tensions.
    Adesida A
    BMC musculoskeletal disorders 14. (2013 Dec 13): 353.

    Decreased hypertrophic differentiation accompanies enhanced matrix formation in co-cultures of outer meniscus cells with bone marrow mesenchymal stromal cells.
    Adesida AB
    Arthritis research and therapy 14.3 (2012 Jun 22): R153.

    Oxygen tension is a determinant of the matrix-forming phenotype of cultured human meniscal fibrochondrocytes.
    Jomha NM
    PloS one 7.6 (2012): e39339.

    Hypoxia mediated isolation and expansion enhances the chondrogenic capacity of bone marrow mesenchymal stromal cells.
    Jomha NM
    Stem cell research and therapy 3.2 (2012 Mar 2): 9.

    Distinct patterns of notochord mineralization in zebrafish coincide with the localization of Osteocalcin isoform 1 during early vertebral centra formation.
    Witten PE
    BMC developmental biology 12. (2012 Oct 9): 28.

    Ret signalling integrates a craniofacial muscle module during development.
    Roehl H
    Development (Cambridge, England) 138.10 (2011 May): 2015-24.

    Regulation of zebrafish skeletogenesis by ext2/dackel and papst1/pinscher.
    Roehl HH
    PLoS genetics 4.7 (2008 Jul 25): e1000136.

    Monoclonal antibodies to connective tissue macromolecules: type II collagen.
    Hendrix MJ
    Biochemical and biophysical research communications 92.2 (1980 Jan 29): 440-6.

    Anatomically shaped tissue-engineered cartilage with tunable and inducible anticytokine delivery for biological joint resurfacing.
    Estes BT
    Proceedings of the National Academy of Sciences of the United States of America 113.31 (2016 Aug 2): E4513-22.

    Polyester type polyHIPE scaffolds with an interconnected porous structure for cartilage regeneration.
    Krajnc P
    Scientific reports 6. (2016 Jun 24): 28695.

    Optimal Seeding Densities for In Vitro Chondrogenesis of Two- and Three-Dimensional-Isolated and -Expanded Bone Marrow-Derived Mesenchymal Stromal Stem Cells Within a Porous Collagen Scaffold.
    Adesida AB
    Tissue engineering. Part C, Methods 22.3 (2016 Mar): 208-20.

    Activin Receptor-Like Kinase Receptors ALK5 and ALK1 Are Both Required for TGFβ-Induced Chondrogenic Differentiation of Human Bone Marrow-Derived Mesenchymal Stem Cells.
    van der Kraan PM
    PloS one 10.12 (2015): e0146124.

    Arthroscopic autologous chondrocyte implantation in the knee with an in situ crosslinking matrix: minimum 4-year clinical results of 15 cases and 1 histological evaluation.
    Richter J
    Archives of orthopaedic and trauma surgery 139.11 (2019 Nov): 1607-1615.

    A study in vivo of the effects of a static compressive load on the proximal tibial physis in rabbits.
    Landis WJ
    The Journal of bone and joint surgery. American volume 94.15 (2012 Aug 1): e1111-10.

    Disparate response of articular- and auricular-derived chondrocytes to oxygen tension.
    Dennis JE
    Connective tissue research 57.4 (2016 Jul): 319-33.

    Yield stress determines bioprintability of hydrogels based on gelatin-methacryloyl and gellan gum for cartilage bioprinting.
    Malda J
    Biofabrication 8.3 (2016 Jul 19): 035003.

    Development of the Synarcual in the Elephant Sharks (Holocephali; Chondrichthyes): Implications for Vertebral Formation and Fusion.
    Trinajstic K
    PloS one 10.9 (2015): e0135138.

    Skeletal callus formation is a nerve-independent regenerative response to limb amputation in mice and Xenopus.
    Endo T
    Regeneration (Oxford, England) 2.4 (2015 Aug): 202-16.

    One-step surgical procedure for the treatment of osteochondral defects with adipose-derived stem cells in a caprine knee defect: a pilot study.
    Helder MN
    BioResearch open access 2.4 (2013 Aug): 315-25.

    Decellularized cartilage-derived matrix as substrate for endochondral bone regeneration.
    Dhert WJ
    Tissue engineering. Part A 21.3-4 (2015 Feb): 694-703.

    Endochondral bone formation in gelatin methacrylamide hydrogel with embedded cartilage-derived matrix particles.
    Malda J
    Biomaterials 37. (2015 Jan): 174-82.

    In vitro induction of alkaline phosphatase levels predicts in vivo bone forming capacity of human bone marrow stromal cells.
    Martens AC
    Stem cell research 12.2 (2014 Mar): 428-40.

    Flow-perfusion interferes with chondrogenic and hypertrophic matrix production by mesenchymal stem cells.
    Gawlitta D
    Journal of biomechanics 47.9 (2014 Jun 27): 2122-9.

    Multipotent Stromal Cells Outperform Chondrocytes on Cartilage-Derived Matrix Scaffolds.
    Malda J
    Cartilage 5.4 (2014 Oct): 221-30.

    Covalent attachment of a three-dimensionally printed thermoplast to a gelatin hydrogel for mechanically enhanced cartilage constructs.
    Malda J
    Acta biomaterialia 10.6 (2014 Jun): 2602-11.

    Hypoxia impedes hypertrophic chondrogenesis of human multipotent stromal cells.
    Dhert WJ
    Tissue engineering. Part A 18.19-20 (2012 Oct): 1957-66.

    Development of a thermosensitive HAMA-containing bio-ink for the fabrication of composite cartilage repair constructs.
    Malda J
    Biofabrication 9.1 (2017 Mar 23): 015026.

    A Synthetic Thermosensitive Hydrogel for Cartilage Bioprinting and Its Biofunctionalization with Polysaccharides.
    Vermonden T
    Biomacromolecules 17.6 (2016 Jun 13): 2137-2147.

    Zonal chondrocyte subpopulations reacquire zone-specific characteristics during in vitro redifferentiation.
    Malda J
    The American journal of sports medicine 37 Suppl 1. (2009 Nov): 97S-104S.

    Cartilage Tissue Engineering: Preventing Tissue Scaffold Contraction Using a 3D-Printed Polymeric Cage.
    van Zuijlen PP
    Tissue engineering. Part C, Methods 22.6 (2016 Jun): 573-84.

    Intact vitreous humor as a potential extracellular matrix hydrogel for cartilage tissue engineering applications.
    Woodfield TBF
    Acta biomaterialia 85. (2019 Feb): 117-130.

    Cartilage regeneration using decellularized cartilage matrix: Long-term comparison of subcutaneous and intranasal placement in a rabbit model.
    Rotter N
    Journal of cranio-maxillo-facial surgery : official publication of the European Association for Cranio-Maxillo-Facial Surgery 47.4 (2019 Apr): 682-694.

    Hydrogel-based delivery of antimiR-221 enhances cartilage regeneration by endogenous cells.
    van Osch GJVM
    Journal of controlled release : official journal of the Controlled Release Society 309. (2019 Sep 10): 220-230.

    Meniscus regeneration combining meniscus and mesenchymal stromal cells in a degradable meniscus implant: an in vitro study.
    Saris DB
    European cells and materials 38. (2019 Aug 12): 51-62.

    FFPE References
    Epitope Map References

    Monoclonal antibodies to connective tissue macromolecules: type II collagen.
    Hendrix MJ
    Biochemical and biophysical research communications 92.2 (1980 Jan 29): 440-6.

    All References

    Distinct patterns of notochord mineralization in zebrafish coincide with the localization of Osteocalcin isoform 1 during early vertebral centra formation.
    Witten PE
    BMC developmental biology 12. (2012 Oct 9): 28.

    Ret signalling integrates a craniofacial muscle module during development.
    Roehl H
    Development (Cambridge, England) 138.10 (2011 May): 2015-24.

    Collagen IX is required for the integrity of collagen II fibrils and the regulation of vascular plexus formation in zebrafish caudal fins.
    Yu J
    Developmental biology 332.2 (2009 Aug 15): 360-70.

    Regulation of zebrafish skeletogenesis by ext2/dackel and papst1/pinscher.
    Roehl HH
    PLoS genetics 4.7 (2008 Jul 25): e1000136.

    Hypoxic culture of bone marrow-derived mesenchymal stromal stem cells differentially enhances in vitro chondrogenesis within cell-seeded collagen and hyaluronic acid porous scaffolds.
    Adesida AB
    Stem cell research and therapy 6. (2015 Apr 23): 84.

    Matrix formation is enhanced in co-cultures of human meniscus cells with bone marrow stromal cells.
    Adesida AB
    Journal of tissue engineering and regenerative medicine 7.12 (2013 Dec): 965-73.

    Matrix forming characteristics of inner and outer human meniscus cells on 3D collagen scaffolds under normal and low oxygen tensions.
    Adesida A
    BMC musculoskeletal disorders 14. (2013 Dec 13): 353.

    Decreased hypertrophic differentiation accompanies enhanced matrix formation in co-cultures of outer meniscus cells with bone marrow mesenchymal stromal cells.
    Adesida AB
    Arthritis research and therapy 14.3 (2012 Jun 22): R153.

    Oxygen tension is a determinant of the matrix-forming phenotype of cultured human meniscal fibrochondrocytes.
    Jomha NM
    PloS one 7.6 (2012): e39339.

    Hypoxia mediated isolation and expansion enhances the chondrogenic capacity of bone marrow mesenchymal stromal cells.
    Jomha NM
    Stem cell research and therapy 3.2 (2012 Mar 2): 9.

    Monoclonal antibodies to connective tissue macromolecules: type II collagen.
    Hendrix MJ
    Biochemical and biophysical research communications 92.2 (1980 Jan 29): 440-6.

    Anatomically shaped tissue-engineered cartilage with tunable and inducible anticytokine delivery for biological joint resurfacing.
    Estes BT
    Proceedings of the National Academy of Sciences of the United States of America 113.31 (2016 Aug 2): E4513-22.

    Polyester type polyHIPE scaffolds with an interconnected porous structure for cartilage regeneration.
    Krajnc P
    Scientific reports 6. (2016 Jun 24): 28695.

    Optimal Seeding Densities for In Vitro Chondrogenesis of Two- and Three-Dimensional-Isolated and -Expanded Bone Marrow-Derived Mesenchymal Stromal Stem Cells Within a Porous Collagen Scaffold.
    Adesida AB
    Tissue engineering. Part C, Methods 22.3 (2016 Mar): 208-20.

    Activin Receptor-Like Kinase Receptors ALK5 and ALK1 Are Both Required for TGFβ-Induced Chondrogenic Differentiation of Human Bone Marrow-Derived Mesenchymal Stem Cells.
    van der Kraan PM
    PloS one 10.12 (2015): e0146124.

    Arthroscopic autologous chondrocyte implantation in the knee with an in situ crosslinking matrix: minimum 4-year clinical results of 15 cases and 1 histological evaluation.
    Richter J
    Archives of orthopaedic and trauma surgery 139.11 (2019 Nov): 1607-1615.

    A study in vivo of the effects of a static compressive load on the proximal tibial physis in rabbits.
    Landis WJ
    The Journal of bone and joint surgery. American volume 94.15 (2012 Aug 1): e1111-10.

    Disparate response of articular- and auricular-derived chondrocytes to oxygen tension.
    Dennis JE
    Connective tissue research 57.4 (2016 Jul): 319-33.

    Yield stress determines bioprintability of hydrogels based on gelatin-methacryloyl and gellan gum for cartilage bioprinting.
    Malda J
    Biofabrication 8.3 (2016 Jul 19): 035003.

    Development of the Synarcual in the Elephant Sharks (Holocephali; Chondrichthyes): Implications for Vertebral Formation and Fusion.
    Trinajstic K
    PloS one 10.9 (2015): e0135138.

    Skeletal callus formation is a nerve-independent regenerative response to limb amputation in mice and Xenopus.
    Endo T
    Regeneration (Oxford, England) 2.4 (2015 Aug): 202-16.

    One-step surgical procedure for the treatment of osteochondral defects with adipose-derived stem cells in a caprine knee defect: a pilot study.
    Helder MN
    BioResearch open access 2.4 (2013 Aug): 315-25.

    Decellularized cartilage-derived matrix as substrate for endochondral bone regeneration.
    Dhert WJ
    Tissue engineering. Part A 21.3-4 (2015 Feb): 694-703.

    Endochondral bone formation in gelatin methacrylamide hydrogel with embedded cartilage-derived matrix particles.
    Malda J
    Biomaterials 37. (2015 Jan): 174-82.

    In vitro induction of alkaline phosphatase levels predicts in vivo bone forming capacity of human bone marrow stromal cells.
    Martens AC
    Stem cell research 12.2 (2014 Mar): 428-40.

    Flow-perfusion interferes with chondrogenic and hypertrophic matrix production by mesenchymal stem cells.
    Gawlitta D
    Journal of biomechanics 47.9 (2014 Jun 27): 2122-9.

    Multipotent Stromal Cells Outperform Chondrocytes on Cartilage-Derived Matrix Scaffolds.
    Malda J
    Cartilage 5.4 (2014 Oct): 221-30.

    Covalent attachment of a three-dimensionally printed thermoplast to a gelatin hydrogel for mechanically enhanced cartilage constructs.
    Malda J
    Acta biomaterialia 10.6 (2014 Jun): 2602-11.

    Hypoxia impedes hypertrophic chondrogenesis of human multipotent stromal cells.
    Dhert WJ
    Tissue engineering. Part A 18.19-20 (2012 Oct): 1957-66.

    Development of a thermosensitive HAMA-containing bio-ink for the fabrication of composite cartilage repair constructs.
    Malda J
    Biofabrication 9.1 (2017 Mar 23): 015026.

    A Synthetic Thermosensitive Hydrogel for Cartilage Bioprinting and Its Biofunctionalization with Polysaccharides.
    Vermonden T
    Biomacromolecules 17.6 (2016 Jun 13): 2137-2147.

    Zonal chondrocyte subpopulations reacquire zone-specific characteristics during in vitro redifferentiation.
    Malda J
    The American journal of sports medicine 37 Suppl 1. (2009 Nov): 97S-104S.

    Cartilage Tissue Engineering: Preventing Tissue Scaffold Contraction Using a 3D-Printed Polymeric Cage.
    van Zuijlen PP
    Tissue engineering. Part C, Methods 22.6 (2016 Jun): 573-84.

    Intact vitreous humor as a potential extracellular matrix hydrogel for cartilage tissue engineering applications.
    Woodfield TBF
    Acta biomaterialia 85. (2019 Feb): 117-130.

    Cartilage regeneration using decellularized cartilage matrix: Long-term comparison of subcutaneous and intranasal placement in a rabbit model.
    Rotter N
    Journal of cranio-maxillo-facial surgery : official publication of the European Association for Cranio-Maxillo-Facial Surgery 47.4 (2019 Apr): 682-694.

    Hydrogel-based delivery of antimiR-221 enhances cartilage regeneration by endogenous cells.
    van Osch GJVM
    Journal of controlled release : official journal of the Controlled Release Society 309. (2019 Sep 10): 220-230.

    Meniscus regeneration combining meniscus and mesenchymal stromal cells in a degradable meniscus implant: an in vitro study.
    Saris DB
    European cells and materials 38. (2019 Aug 12): 51-62.

    Joint Development Involves a Continuous Influx of Gdf5-Positive Cells.
    Zelzer E
    Cell reports 15.12 (2016 Jun 21): 2577-87.

    Molecular footprinting of skeletal tissues in the catshark Scyliorhinus canicula and the clawed frog Xenopus tropicalis identifies conserved and derived features of vertebrate calcification.
    Debiais-Thibaud M
    Frontiers in genetics 6. (2015): 283.

    Skeletal plasticity in response to embryonic muscular activity underlies the development and evolution of the perching digit of birds.
    Vargas AO
    Scientific reports 5. (2015 May 14): 9840.

    Reconstruction of an in vitro tissue-specific microenvironment to rejuvenate synovium-derived stem cells for cartilage tissue engineering.
    Pei M
    Tissue engineering. Part A 15.12 (2009 Dec): 3809-21.

    Collagen of accessory structures of organ of Corti.
    Thalmann I
    Connective tissue research 29.3 (1993): 191-201.

    Engineering of functional cartilage tissue using stem cells from synovial lining: a preliminary study.
    Vunjak-Novakovic G
    Clinical orthopaedics and related research 466.8 (2008 Aug): 1880-9.

    In-vivo generation of bone via endochondral ossification by in-vitro chondrogenic priming of adult human and rat mesenchymal stem cells.
    van Osch GJ
    BMC musculoskeletal disorders 12. (2011 Jan 31): 31.

    Adipose tissue-derived mesenchymal stem cell yield and growth characteristics are affected by the tissue-harvesting procedure.
    van Milligen FJ
    Cytotherapy 8.2 (2006): 166-77.

    Localized, direct plasmid gene delivery in vivo: prolonged therapy results in reproducible tissue regeneration.
    Goldstein S
    Nature medicine 5.7 (1999 Jul): 753-9.

    Identification of cancer stem cells in Ewing's sarcoma.
    Stamenkovic I
    Cancer research 69.5 (2009 Mar 1): 1776-81.

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