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SKU: 2E9
View product citations for antibody 2E9 on CiteAb

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Available: 168

DSHB Data Sheet

Catalog Fields

Antigen: Myosin heavy chain, neonatal fast
Hybridoma Cells Available: Yes
Antigen Species: chicken
Depositor: Bandman, E.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: University of California, Davis
Positive Tested Species Reactivity: Chicken
Depositors Notes:
Antigen Molecular Weight: 220 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Immunogen: protein (isolated from chicken muscle, after denature PAGE, the stained band was cut out from gel and used as immunogen)
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: SP2/0
Epitope Location or Sequence:
Uniprot ID:  
Immunogen Sequence:
Entrez Gene ID:  
Additional Characterization:  
Antibody Registry ID: AB_2101116 
Additional Information:
Recommended Applications: Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
2E9 was deposited to the DSHB by Bandman, E. (DSHB Hybridoma Product 2E9)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
All cell products contain the antimicrobial ProClin. Click here for additional information.

1 Reference

  • Initial Publication
  • All References
  • Initial Publication

    Continued expression of neonatal myosin heavy chain in adult dystrophic skeletal muscle.
    Bandman E
    Science (New York, N.Y.) 227.4688 (1985 Feb 15): 780-2.

    All References

    Continued expression of neonatal myosin heavy chain in adult dystrophic skeletal muscle.
    Bandman E
    Science (New York, N.Y.) 227.4688 (1985 Feb 15): 780-2.

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