PAX7

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$40.00
SKU: PAX7
View product citations for antibody PAX7 on CiteAb

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Available: 580

DSHB Data Sheet

Catalog Fields

Antigen: Pax7
Hybridoma Cells Available: Yes
Antigen Species: Chicken
Depositor: Kawakami, A.
Isotype: MIgG1, kappa light chain
Antigen Sequence:
Host Species: mouse
Depositors Institution: Tokyo Institute of Technology
Positive Tested Species Reactivity: Amphibian, Avian, Bovine, Canine, Fish, Goat, Human, Mouse, Ovine, Porcine, Rat, Turtle, Xenopus, Zebrafish
Depositors Notes: This antibody recognizes a transcription factor in regulation of myogenesis.
Antigen Molecular Weight: Predicted: 57.5 kDa; Apparent: 50, 53 and 55 kDa
Human Protein Atlas:
Predicted Species Reactivity:  
Gene: Pax7
Immunogen: Recombinant protein (C-terminal region, aa 352-523)
Alternate Gene Names: PAX-7
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: P3U1
Epitope Location or Sequence: C-terminal region, aa 418-427
Uniprot ID: O42349 
Immunogen Sequence: Partial protein
Entrez Gene ID: 395942 
Additional Characterization:
Antibody Registry ID: AB_528428 
Additional Information: This antibody recognizes the nuclei in adult skeletal muscle satellite cells
Recommended Applications: Chromatin Immunoprecipitation, FACS, FFPE, Gel Supershift, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
PAX7 was deposited to the DSHB by Kawakami, A. (DSHB Hybridoma Product PAX7)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

73 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • IP References
  • FFPE References
  • FACS References
  • Epitope Map References
  • Gel Supershift References
  • All References
  • Initial Publication
    IF References
    WB References
    IHC References
    IP References
    FFPE References
    FACS References
    Epitope Map References
    Gel Supershift References
    All References

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