Hybridoma Cells Available: Yes
Antigen Species: Chicken
Depositor: Kawakami, A.
Isotype: MIgG1, kappa light chain
Host Species: mouse
Depositors Institution: Tokyo Institute of Technology
Positive Tested Species Reactivity: Amphibian, Avian, Bovine, Canine, Fish, Goat, Human, Mouse, Ovine, Porcine, Rat, Turtle, Xenopus, Zebrafish
Depositors Notes: This antibody recognizes a transcription factor in regulation of myogenesis.
Antigen Molecular Weight: Predicted: 57.5 kDa; Apparent: 50, 53 and 55 kDa
Human Protein Atlas:
Predicted Species Reactivity:
Immunogen: Recombinant protein (C-terminal region, aa 352-523)
Alternate Gene Names: PAX-7
Alternate Antibody Name:
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: P3U1
Epitope Location or Sequence: C-terminal region, aa 418-427
Uniprot ID: O42349
Immunogen Sequence: Partial protein
Entrez Gene ID: 395942
Antibody Registry ID: AB_528428
Additional Information: This antibody recognizes the nuclei in adult skeletal muscle satellite cells
Recommended Applications: Chromatin Immunoprecipitation, FACS, FFPE, Gel Supershift, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
PAX7 was deposited to the DSHB by Kawakami, A. (DSHB Hybridoma Product PAX7)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
Epitope Map References
Gel Supershift References