3F11

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$40.00
SKU: 3F11
View product citations for antibody 3F11 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: Tyrosine-protein phosphatase 69D
Hybridoma Cells Available: Yes
Antigen Species: Drosophila
Depositor: Zinn, K.
Isotype: MIgG1, kappa light chain
Antigen Sequence:
Host Species: mouse
Depositors Institution: California Institute of Technology
Positive Tested Species Reactivity: Drosophila
Depositors Notes: See PMID: 25528559 for 3F11 fixation optimization.
Antigen Molecular Weight: 116 kDa
Human Protein Atlas:
Predicted Species Reactivity:  
Gene: Ptp69D
Immunogen: Recombinant protein domain
Alternate Gene Names: DPTP, Dmel_CG10975
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: HL-1
Epitope Location or Sequence: N-terminus of membrane-proximal region of PTP69D
Uniprot ID: P16620 
Immunogen Sequence: Extracellular domain
Entrez Gene ID: 39443 
Additional Characterization:
Antibody Registry ID: AB_528444 
Additional Information: RRID:AB_528444. FBgn0014007.
Recommended Applications: Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
3F11 was deposited to the DSHB by Zinn, K. (DSHB Hybridoma Product 3F11)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

8 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • IP References
  • Epitope Map References
  • All References
  • Initial Publication

    A Drosophila receptor tyrosine phosphatase expressed in the embryonic CNS and larval optic lobes is a member of the set of proteins bearing the "HRP" carbohydrate epitope.
    Zinn K
    The Journal of neuroscience : the official journal of the Society for Neuroscience 14.12 (1994 Dec): 7272-83.

    IF References
    WB References

    A Drosophila receptor tyrosine phosphatase expressed in the embryonic CNS and larval optic lobes is a member of the set of proteins bearing the "HRP" carbohydrate epitope.
    Zinn K
    The Journal of neuroscience : the official journal of the Society for Neuroscience 14.12 (1994 Dec): 7272-83.

    IHC References
    IP References

    A Drosophila receptor tyrosine phosphatase expressed in the embryonic CNS and larval optic lobes is a member of the set of proteins bearing the "HRP" carbohydrate epitope.
    Zinn K
    The Journal of neuroscience : the official journal of the Society for Neuroscience 14.12 (1994 Dec): 7272-83.

    Epitope Map References
    All References

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