4F3 anti-discs large

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SKU: 4F3 anti-discs large
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DSHB Data Sheet

Catalog Fields

Antigen: Discs large (Drosophila)
Hybridoma Cells Available: Yes
Antigen Species: Drosophila
Depositor: Goodman, C.
Isotype: MIgG1
Depositors Institution: University of California, Berkeley
Host Species: mouse
Gene: dlg1
Positive Tested Species Reactivity: Drosophila
Alternate Gene Names: DLG; DLG-A; DLG1; DlgA; DlgS97; Dmel\CG1725;
Antigen Molecular Weight: Predicted: 106.7 kDa
Clonality: Monoclonal
Immunogen: GST-PDZ2-GST fusion protein; recombinant.
Epitope Mapped: Yes
Alternate Antibody Name: discs large 1, isoform L
Epitope Location or Sequence: a.a 439–756
Myeloma Strain: P3 x 63 Ag8.653
Immunogen Sequence: same as the antigen
Uniprot ID: P31007 
Additional Information: RRID: AB_528203
Entrez Gene ID: 32083 
Antibody Registry ID: AB_528203 
Recommended Applications: Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
4F3 anti-discs large was deposited to the DSHB by Goodman, C. (DSHB Hybridoma Product 4F3 anti-discs large)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

53 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • IP References
  • All References
  • Initial Publication
    IF References

    glaikit is essential for the formation of epithelial polarity and neuronal development.
    Tear G
    Current biology : CB 14.22 (2004 Nov 23): 2039-45.

    External and circadian inputs modulate synaptic protein expression in the visual system of Drosophila melanogaster.
    Pyza EM
    Frontiers in physiology 5. (2014): 102.

    Formation of a Bazooka-Stardust complex is essential for plasma membrane polarity in epithelia.
    Wodarz A
    The Journal of cell biology 190.5 (2010 Sep 6): 751-60.

    Loss of the spectraplakin short stop activates the DLK injury response pathway in Drosophila.
    DiAntonio A
    The Journal of neuroscience : the official journal of the Society for Neuroscience 33.45 (2013 Nov 6): 17863-73.

    Stringent analysis of gene function and protein-protein interactions using fluorescently tagged genes.
    Perrimon N
    Genetics 190.3 (2012 Mar): 931-40.

    Three Drosophila liprins interact to control synapse formation.
    Treisman JE
    The Journal of neuroscience : the official journal of the Society for Neuroscience 30.46 (2010 Nov 17): 15358-68.

    Tuberous sclerosis complex regulates Drosophila neuromuscular junction growth via the TORC2/Akt pathway.
    Wairkar YP
    Human molecular genetics 22.10 (2013 May 15): 2010-23.

    Neurofibromin mediates FAK signaling in confining synapse growth at Drosophila neuromuscular junctions.
    Chien CT
    The Journal of neuroscience : the official journal of the Society for Neuroscience 32.47 (2012 Nov 21): 16971-81.

    Subcellular mapping of dendritic activity in optic flow processing neurons.
    Haag J
    Journal of comparative physiology. A, Neuroethology, sensory, neural, and behavioral physiology 200.5 (2014 May): 359-70.

    Drosophila cyfip regulates synaptic development and endocytosis by suppressing filamentous actin assembly.
    Zhang YQ
    PLoS genetics 9.4 (2013 Apr): e1003450.

    Synaptic abnormalities in a Drosophila model of Alzheimer's disease.
    Marenda DR
    Disease models & mechanisms 7.3 (2014 Mar): 373-85.

    Detection and differentiation of picornaviruses in clinical samples following genomic amplification.
    Al-Nakib W
    The Journal of general virology 71 ( Pt 9). (1990 Sep): 2141-7.

    Dbo/Henji Modulates Synaptic dPAK to Gate Glutamate Receptor Abundance and Postsynaptic Response.
    Chien CT
    PLoS genetics 12.10 (2016 Oct): e1006362.

    Drak Is Required for Actomyosin Organization During Drosophila Cellularization.
    Thomas JH
    G3 (Bethesda, Md.) 6.4 (2016 Apr 7): 819-28.

    Fragile X Mental Retardation Protein Requirements in Activity-Dependent Critical Period Neural Circuit Refinement.
    Broadie K
    Current biology : CB 27.15 (2017 Aug 7): 2318-2330.e3.

    Dietary restriction improves intestinal cellular fitness to enhance gut barrier function and lifespan in D. melanogaster.
    Kapahi P
    PLoS genetics 14.11 (2018 Nov): e1007777.

    C-terminal Src kinase (Csk) regulates the tricellular junction protein Gliotactin independent of Src.
    Auld VJ
    Molecular biology of the cell 29.2 (2018 Jan 15): 123-136.

    A new Drosophila model of Ubiquilin knockdown shows the effect of impaired proteostasis on locomotive and learning abilities.
    Yamaguchi M
    Experimental cell research 362.2 (2018 Jan 15): 461-471.

    Diversity and wiring variability of visual local neurons in the Drosophila medulla M6 stratum.
    Chiang AS
    The Journal of comparative neurology 522.17 (2014 Dec 1): 3795-816.

    Squamous Cell Carcinoma-related Oncogene (SCCRO) Family Members Regulate Cell Growth and Proliferation through Their Cooperative and Antagonistic Effects on Cullin Neddylation.
    Singh B
    The Journal of biological chemistry 291.12 (2016 Mar 18): 6200-17.

    Dendritic Integration of Sensory Evidence in Perceptual Decision-Making.
    Miesenböck G
    Cell 173.4 (2018 May 3): 894-905.e13.

    Distinct homeostatic modulations stabilize reduced postsynaptic receptivity in response to presynaptic DLK signaling.
    Dickman D
    Nature communications 9.1 (2018 May 10): 1856.

    Two temporal functions of Glass: Ommatidium patterning and photoreceptor differentiation.
    Zelhof AC
    Developmental biology 414.1 (2016 Jun 1): 4-20.

    Neuropeptides Modulate Female Chemosensory Processing upon Mating in Drosophila.
    Grunwald Kadow IC
    PLoS biology 14.5 (2016 May): e1002455.

    Tao Negatively Regulates BMP Signaling During Neuromuscular Junction Development in Drosophila.
    Vanderzalm PJ
    Developmental neurobiology 79.4 (2019 Apr): 335-349.

    Splicing repression is a major function of TDP-43 in motor neurons.
    Wong PC
    Acta neuropathologica 138.5 (2019 Nov): 813-826.

    Structure-function analysis of β-arrestin Kurtz reveals a critical role of receptor interactions in downregulation of GPCR signaling in vivo.
    Veraksa A
    Developmental biology 455.2 (2019 Nov 15): 409-419.

    A circuit-dependent ROS feedback loop mediates glutamate excitotoxicity to sculpt the <i>Drosophila</i> motor system.
    Yao CK
    eLife 8. (2019 Jul 18): .

    Select Septate Junction Proteins Direct ROS-Mediated Paracrine Regulation of Drosophila Cardiac Function.
    Wang W
    Cell reports 28.6 (2019 Aug 6): 1455-1470.e4.

    Two Pathways for the Activity-Dependent Growth and Differentiation of Synaptic Boutons in <i>Drosophila</i>.
    Bykhovskaia M
    eNeuro 6.4 (2019 Jul/Aug): .

    Characterization of TNF-induced cell death in Drosophila reveals caspase- and JNK-dependent necrosis and its role in tumor suppression.
    Fan Y
    Cell death & disease 10.8 (2019 Aug 14): 613.

    WB References

    Genetic analysis of synaptotagmin C2 domain specificity in regulating spontaneous and evoked neurotransmitter release.
    Littleton JT
    The Journal of neuroscience : the official journal of the Society for Neuroscience 33.1 (2013 Jan 2): 187-200.

    External and circadian inputs modulate synaptic protein expression in the visual system of Drosophila melanogaster.
    Pyza EM
    Frontiers in physiology 5. (2014): 102.

    Using Drosophila as an integrated model to study mild repetitive traumatic brain injury.
    Ratliff EP
    Scientific reports 6. (2016 May 4): 25252.

    Three-dimensional reconstruction of brain-wide wiring networks in Drosophila at single-cell resolution.
    Hwang JK
    Current biology : CB 21.1 (2011 Jan 11): 1-11.

    Drosophila adducin regulates Dlg phosphorylation and targeting of Dlg to the synapse and epithelial membrane.
    Krieger C
    Developmental biology 357.2 (2011 Sep 15): 392-403.

    DMob4/Phocein regulates synapse formation, axonal transport, and microtubule organization.
    Littleton JT
    The Journal of neuroscience : the official journal of the Society for Neuroscience 30.15 (2010 Apr 14): 5189-203.

    Drosophila Neto is essential for clustering glutamate receptors at the neuromuscular junction.
    Serpe M
    Genes & development 26.9 (2012 May 1): 974-87.

    Gliotactin and Discs large form a protein complex at the tricellular junction of polarized epithelial cells in Drosophila.
    Auld VJ
    Journal of cell science 119.Pt 21 (2006 Nov 1): 4391-401.

    IHC References

    Regulation of postsynaptic structure and protein localization by the Rho-type guanine nucleotide exchange factor dPix.
    Goodman CS
    Neuron 32.3 (2001 Nov 8): 415-24.

    LRRK2 kinase regulates synaptic morphology through distinct substrates at the presynaptic and postsynaptic compartments of the Drosophila neuromuscular junction.
    Lu B
    The Journal of neuroscience : the official journal of the Society for Neuroscience 30.50 (2010 Dec 15): 16959-69.

    Rab3 dynamically controls protein composition at active zones.
    DiAntonio A
    Neuron 64.5 (2009 Dec 10): 663-77.

    Stathmin is required for stability of the Drosophila neuromuscular junction.
    DiAntonio A
    The Journal of neuroscience : the official journal of the Society for Neuroscience 31.42 (2011 Oct 19): 15026-34.

    Formation of a Bazooka-Stardust complex is essential for plasma membrane polarity in epithelia.
    Wodarz A
    The Journal of cell biology 190.5 (2010 Sep 6): 751-60.

    Drosophila adducin regulates Dlg phosphorylation and targeting of Dlg to the synapse and epithelial membrane.
    Krieger C
    Developmental biology 357.2 (2011 Sep 15): 392-403.

    Subcellular mapping of dendritic activity in optic flow processing neurons.
    Haag J
    Journal of comparative physiology. A, Neuroethology, sensory, neural, and behavioral physiology 200.5 (2014 May): 359-70.

    The Tricellular Junction Protein Sidekick Regulates Vertex Dynamics to Promote Bicellular Junction Extension.
    Kuranaga E
    Developmental cell 50.3 (2019 Aug 5): 327-338.e5.

    IP References
    All References

    Regulation of postsynaptic structure and protein localization by the Rho-type guanine nucleotide exchange factor dPix.
    Goodman CS
    Neuron 32.3 (2001 Nov 8): 415-24.

    LRRK2 kinase regulates synaptic morphology through distinct substrates at the presynaptic and postsynaptic compartments of the Drosophila neuromuscular junction.
    Lu B
    The Journal of neuroscience : the official journal of the Society for Neuroscience 30.50 (2010 Dec 15): 16959-69.

    Rab3 dynamically controls protein composition at active zones.
    DiAntonio A
    Neuron 64.5 (2009 Dec 10): 663-77.

    Stathmin is required for stability of the Drosophila neuromuscular junction.
    DiAntonio A
    The Journal of neuroscience : the official journal of the Society for Neuroscience 31.42 (2011 Oct 19): 15026-34.

    Formation of a Bazooka-Stardust complex is essential for plasma membrane polarity in epithelia.
    Wodarz A
    The Journal of cell biology 190.5 (2010 Sep 6): 751-60.

    Drosophila adducin regulates Dlg phosphorylation and targeting of Dlg to the synapse and epithelial membrane.
    Krieger C
    Developmental biology 357.2 (2011 Sep 15): 392-403.

    Subcellular mapping of dendritic activity in optic flow processing neurons.
    Haag J
    Journal of comparative physiology. A, Neuroethology, sensory, neural, and behavioral physiology 200.5 (2014 May): 359-70.

    The Tricellular Junction Protein Sidekick Regulates Vertex Dynamics to Promote Bicellular Junction Extension.
    Kuranaga E
    Developmental cell 50.3 (2019 Aug 5): 327-338.e5.

    glaikit is essential for the formation of epithelial polarity and neuronal development.
    Tear G
    Current biology : CB 14.22 (2004 Nov 23): 2039-45.

    External and circadian inputs modulate synaptic protein expression in the visual system of Drosophila melanogaster.
    Pyza EM
    Frontiers in physiology 5. (2014): 102.

    Loss of the spectraplakin short stop activates the DLK injury response pathway in Drosophila.
    DiAntonio A
    The Journal of neuroscience : the official journal of the Society for Neuroscience 33.45 (2013 Nov 6): 17863-73.

    Stringent analysis of gene function and protein-protein interactions using fluorescently tagged genes.
    Perrimon N
    Genetics 190.3 (2012 Mar): 931-40.

    Three Drosophila liprins interact to control synapse formation.
    Treisman JE
    The Journal of neuroscience : the official journal of the Society for Neuroscience 30.46 (2010 Nov 17): 15358-68.

    Tuberous sclerosis complex regulates Drosophila neuromuscular junction growth via the TORC2/Akt pathway.
    Wairkar YP
    Human molecular genetics 22.10 (2013 May 15): 2010-23.

    Neurofibromin mediates FAK signaling in confining synapse growth at Drosophila neuromuscular junctions.
    Chien CT
    The Journal of neuroscience : the official journal of the Society for Neuroscience 32.47 (2012 Nov 21): 16971-81.

    Drosophila cyfip regulates synaptic development and endocytosis by suppressing filamentous actin assembly.
    Zhang YQ
    PLoS genetics 9.4 (2013 Apr): e1003450.

    Synaptic abnormalities in a Drosophila model of Alzheimer's disease.
    Marenda DR
    Disease models & mechanisms 7.3 (2014 Mar): 373-85.

    Detection and differentiation of picornaviruses in clinical samples following genomic amplification.
    Al-Nakib W
    The Journal of general virology 71 ( Pt 9). (1990 Sep): 2141-7.

    Dbo/Henji Modulates Synaptic dPAK to Gate Glutamate Receptor Abundance and Postsynaptic Response.
    Chien CT
    PLoS genetics 12.10 (2016 Oct): e1006362.

    Drak Is Required for Actomyosin Organization During Drosophila Cellularization.
    Thomas JH
    G3 (Bethesda, Md.) 6.4 (2016 Apr 7): 819-28.

    Fragile X Mental Retardation Protein Requirements in Activity-Dependent Critical Period Neural Circuit Refinement.
    Broadie K
    Current biology : CB 27.15 (2017 Aug 7): 2318-2330.e3.

    Dietary restriction improves intestinal cellular fitness to enhance gut barrier function and lifespan in D. melanogaster.
    Kapahi P
    PLoS genetics 14.11 (2018 Nov): e1007777.

    C-terminal Src kinase (Csk) regulates the tricellular junction protein Gliotactin independent of Src.
    Auld VJ
    Molecular biology of the cell 29.2 (2018 Jan 15): 123-136.

    A new Drosophila model of Ubiquilin knockdown shows the effect of impaired proteostasis on locomotive and learning abilities.
    Yamaguchi M
    Experimental cell research 362.2 (2018 Jan 15): 461-471.

    Diversity and wiring variability of visual local neurons in the Drosophila medulla M6 stratum.
    Chiang AS
    The Journal of comparative neurology 522.17 (2014 Dec 1): 3795-816.

    Squamous Cell Carcinoma-related Oncogene (SCCRO) Family Members Regulate Cell Growth and Proliferation through Their Cooperative and Antagonistic Effects on Cullin Neddylation.
    Singh B
    The Journal of biological chemistry 291.12 (2016 Mar 18): 6200-17.

    Dendritic Integration of Sensory Evidence in Perceptual Decision-Making.
    Miesenböck G
    Cell 173.4 (2018 May 3): 894-905.e13.

    Distinct homeostatic modulations stabilize reduced postsynaptic receptivity in response to presynaptic DLK signaling.
    Dickman D
    Nature communications 9.1 (2018 May 10): 1856.

    Two temporal functions of Glass: Ommatidium patterning and photoreceptor differentiation.
    Zelhof AC
    Developmental biology 414.1 (2016 Jun 1): 4-20.

    Neuropeptides Modulate Female Chemosensory Processing upon Mating in Drosophila.
    Grunwald Kadow IC
    PLoS biology 14.5 (2016 May): e1002455.

    Tao Negatively Regulates BMP Signaling During Neuromuscular Junction Development in Drosophila.
    Vanderzalm PJ
    Developmental neurobiology 79.4 (2019 Apr): 335-349.

    Splicing repression is a major function of TDP-43 in motor neurons.
    Wong PC
    Acta neuropathologica 138.5 (2019 Nov): 813-826.

    Structure-function analysis of β-arrestin Kurtz reveals a critical role of receptor interactions in downregulation of GPCR signaling in vivo.
    Veraksa A
    Developmental biology 455.2 (2019 Nov 15): 409-419.

    A circuit-dependent ROS feedback loop mediates glutamate excitotoxicity to sculpt the <i>Drosophila</i> motor system.
    Yao CK
    eLife 8. (2019 Jul 18): .

    Select Septate Junction Proteins Direct ROS-Mediated Paracrine Regulation of Drosophila Cardiac Function.
    Wang W
    Cell reports 28.6 (2019 Aug 6): 1455-1470.e4.

    Two Pathways for the Activity-Dependent Growth and Differentiation of Synaptic Boutons in <i>Drosophila</i>.
    Bykhovskaia M
    eNeuro 6.4 (2019 Jul/Aug): .

    Characterization of TNF-induced cell death in Drosophila reveals caspase- and JNK-dependent necrosis and its role in tumor suppression.
    Fan Y
    Cell death & disease 10.8 (2019 Aug 14): 613.

    Genetic analysis of synaptotagmin C2 domain specificity in regulating spontaneous and evoked neurotransmitter release.
    Littleton JT
    The Journal of neuroscience : the official journal of the Society for Neuroscience 33.1 (2013 Jan 2): 187-200.

    Using Drosophila as an integrated model to study mild repetitive traumatic brain injury.
    Ratliff EP
    Scientific reports 6. (2016 May 4): 25252.

    Three-dimensional reconstruction of brain-wide wiring networks in Drosophila at single-cell resolution.
    Hwang JK
    Current biology : CB 21.1 (2011 Jan 11): 1-11.

    DMob4/Phocein regulates synapse formation, axonal transport, and microtubule organization.
    Littleton JT
    The Journal of neuroscience : the official journal of the Society for Neuroscience 30.15 (2010 Apr 14): 5189-203.

    Drosophila Neto is essential for clustering glutamate receptors at the neuromuscular junction.
    Serpe M
    Genes & development 26.9 (2012 May 1): 974-87.

    Gliotactin and Discs large form a protein complex at the tricellular junction of polarized epithelial cells in Drosophila.
    Auld VJ
    Journal of cell science 119.Pt 21 (2006 Nov 1): 4391-401.

    Drosophila motor neuron retraction during metamorphosis is mediated by inputs from TGF-β/BMP signaling and orphan nuclear receptors.
    Dura JM
    PloS one 7.7 (2012): e40255.

    Apical localisation of crumbs in the boundary cells of the Drosophila hindgut is independent of its canonical interaction partner stardust.
    Knust E
    PloS one 9.4 (2014): e94038.

    The kinesin-3, unc-104 regulates dendrite morphogenesis and synaptic development in Drosophila.
    Rasse TM
    Genetics 195.1 (2013 Sep): 59-72.

    Drosophila twinfilin is required for cell migration and synaptic endocytosis.
    Zhang YQ
    Journal of cell science 123.Pt 9 (2010 May 1): 1546-56.

    Involvement of Lgl and Mahjong/VprBP in cell competition.
    Fujita Y
    PLoS biology 8.7 (2010 Jul 13): e1000422.

    Brain tumor regulates neuromuscular synapse growth and endocytosis in Drosophila by suppressing mad expression.
    Zhang YQ
    The Journal of neuroscience : the official journal of the Society for Neuroscience 33.30 (2013 Jul 24): 12352-63.

    Crumbs controls epithelial integrity by inhibiting Rac1 and PI3K.
    Laprise P
    Journal of cell science 124.Pt 20 (2011 Oct 15): 3393-8.

    Congenital corneal anaesthesia and the MURCS association: a case report.
    Yates JR
    The British journal of ophthalmology 72.3 (1988 Mar): 236-8.

    Retrograde BMP signaling controls synaptic growth at the NMJ by regulating trio expression in motor neurons.
    Haghighi AP
    Neuron 66.4 (2010 May 27): 536-49.

    Neuroligin 2 is required for synapse development and function at the Drosophila neuromuscular junction.
    Xie W
    The Journal of neuroscience : the official journal of the Society for Neuroscience 31.2 (2011 Jan 12): 687-99.

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