Antigen: Discs large (Drosophila)
Hybridoma Cells Available: Yes
Antigen Species: Drosophila
Depositor: Goodman, C.
Host Species: mouse
Depositors Institution: University of California, Berkeley
Positive Tested Species Reactivity: Drosophila
Antigen Molecular Weight: Predicted: 106.7 kDa
Human Protein Atlas:
Predicted Species Reactivity:
Immunogen: GST-PDZ2-GST fusion protein; recombinant.
Alternate Gene Names: DLG; DLG-A; DLG1; DlgA; DlgS97; Dmel\CG1725;
Alternate Antibody Name: discs large 1, isoform L
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: P3 x 63 Ag8.653
Epitope Location or Sequence: a.a 439–756
Uniprot ID: P31007
Immunogen Sequence: same as the antigen
Entrez Gene ID: 32083
Antibody Registry ID: AB_528203
Additional Information: RRID: AB_528203
Recommended Applications: Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
4F3 anti-discs large was deposited to the DSHB by Goodman, C. (DSHB Hybridoma Product 4F3 anti-discs large)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).