3F2/D8

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$40.00
SKU: 3F2/D8
View product citations for antibody 3F2/D8 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: Podocalyxin/gp135
Hybridoma Cells Available: Yes
Antigen Species: canine
Depositor: Ojakian, G.K.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: State University of New York, Brooklyn
Positive Tested Species Reactivity: Canine
Depositors Notes: The podocalyxin epitope withstands 4% paraformaldehyde/0.1% glutaraldehyde fixation but not paraffin processing.
Antigen Molecular Weight: 135 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: PODXL
Immunogen: Intact Madin-Darby canine kidney (MDCK) cells in adjuvant
Alternate Gene Names: PCLP,PCLP1
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: P3U-1
Epitope Location or Sequence: Extracellular N-terminus 59 a.a. following signal recognition peptide
Uniprot ID: Q52S86 
Immunogen Sequence: Full length protein
Entrez Gene ID: 482252 
Additional Characterization:  
Antibody Registry ID: AB_2618385 
Additional Information: RRID:AB_2618385
Recommended Applications: ELISA, FFPE, Immunofluorescence, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
3F2/D8 was deposited to the DSHB by Ojakian, G.K. (DSHB Hybridoma Product 3F2/D8)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

8 References

  • Initial Publication
  • IF References
  • WB References
  • IP References
  • ELISA References
  • Epitope Map References
  • All References
  • Initial Publication

    The MDCK epithelial cell line expresses a cell surface antigen of the kidney distal tubule.
    Ojakian GK
    The Journal of cell biology 93.2 (1982 May): 269-77.

    IF References
    WB References
    IP References
    ELISA References
    Epitope Map References

    Molecular identification of canine podocalyxin-like protein 1 as a renal tubulogenic regulator.
    Jou TS
    Journal of the American Society of Nephrology : JASN 16.6 (2005 Jun): 1612-22.

    All References

    The MDCK epithelial cell line expresses a cell surface antigen of the kidney distal tubule.
    Ojakian GK
    The Journal of cell biology 93.2 (1982 May): 269-77.

    The polarized distribution of an apical cell surface glycoprotein is maintained by interactions with the cytoskeleton of Madin-Darby canine kidney cells.
    Schwimmer R
    The Journal of cell biology 107.6 Pt 1 (1988 Dec): 2377-87.

    Polarized insertion of an intracellular glycoprotein pool into the apical membrane of MDCK cells.
    Herz RE
    The American journal of physiology 258.3 Pt 1 (1990 Mar): C390-8.

    Steps in the morphogenesis of a polarized epithelium. I. Uncoupling the roles of cell-cell and cell-substratum contact in establishing plasma membrane polarity in multicellular epithelial (MDCK) cysts.
    Nelson WJ
    Journal of cell science 95 ( Pt 1). (1990 Jan): 137-51.

    Gp135/podocalyxin and NHERF-2 participate in the formation of a preapical domain during polarization of MDCK cells.
    Füllekrug J
    The Journal of cell biology 168.2 (2005 Jan 17): 303-13.

    Large-scale identification of calcium oxalate monohydrate crystal-binding proteins on apical membrane of distal renal tubular epithelial cells.
    Thongboonkerd V
    Journal of proteome research 10.10 (2011 Oct 7): 4463-77.

    Hyperactivity of Mek in TNS1 knockouts leads to potential treatments for cystic kidney diseases.
    Lo SH
    Cell death & disease 10.12 (2019 Nov 18): 871.

    Molecular identification of canine podocalyxin-like protein 1 as a renal tubulogenic regulator.
    Jou TS
    Journal of the American Society of Nephrology : JASN 16.6 (2005 Jun): 1612-22.

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