R26.4C
Catalog Fields
Clone ID/Product Name: R26.4C
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Symbol: Tjp1
Ab Isotype: RIgG1, kappa light chain
Gene Name:
Antibody Registry ID: AB_2205518
Uniprot ID: P39447
Entrez Gene ID: 292994
Clonality: Monoclonal
Immunogen: Isolated deoxycholate-extracted plasma membrane junctional regions from liver
Clone:
Immunogen Sequence:
Myeloma Strain: P3x63 Ag8U.1 (CRL 1597)
Epitope Mapped: No
Antigen Name: ZO-1; tight junction protein 1
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 3/29/1989
Antigen Molecular Weight: 194kD Predicted; 225k kD on SDS-PAGE
Depositor: Goodenough, D.A.
Antigen Sequence:
Depositor Institution: Harvard Medical School
Antigen Species: Mouse
Depositor Notes: The epitope bound by this antibody is on the cytoplasmic face of plasma membrane at tight junctions.
Host Species: rat
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Canine, Fish, Mouse, Porcine, Rat, Zebrafish
Additional Information:
Recommended Applications: Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
R26.4C was deposited to the DSHB by Goodenough, D.A. (DSHB Hybridoma Product R26.4C)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
24 References
Initial Publication
Identification of ZO-1: a high molecular weight polypeptide associated with the tight junction (zonula occludens) in a variety of epithelia.
Goodenough DA
The Journal of cell biology 103.3 (1986 Sep): 755-66.