R26.4C

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$40.00
SKU: R26.4C
View product citations for antibody R26.4C on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: ZO-1; tight junction protein 1
Hybridoma Cells Available: Yes
Antigen Species: Mouse
Depositor: Goodenough, D.A.
Isotype: RIgG1, kappa light chain
Antigen Sequence:
Host Species: rat
Depositors Institution: Harvard Medical School
Positive Tested Species Reactivity: Canine, Fish, Mouse, Porcine, Rat, Zebrafish
Depositors Notes: The epitope bound by this antibody is on the cytoplasmic face of plasma membrane at tight junctions.
Antigen Molecular Weight: 194kD Predicted; 225k kD on SDS-PAGE
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: Tjp1
Immunogen: Isolated deoxycholate-extracted plasma membrane junctional regions from liver
Alternate Gene Names: Zo1
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: P3x63 Ag8U.1 (CRL 1597)
Epitope Location or Sequence:
Uniprot ID: F1M4A0 
Immunogen Sequence:
Entrez Gene ID: 292994 
Additional Characterization:  
Antibody Registry ID: AB_2205518 
Additional Information:
Recommended Applications: Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
R26.4C was deposited to the DSHB by Goodenough, D.A. (DSHB Hybridoma Product R26.4C)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

24 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • IP References
  • FFPE References
  • All References
  • Initial Publication
    IF References
    WB References
    IHC References

    Enteroendocrine cells are specifically marked by cell surface expression of claudin-4 in mouse small intestine.
    Hamazaki Y
    PloS one 9.6 (2014): e90638.

    Filamin A regulates neuronal migration through brefeldin A-inhibited guanine exchange factor 2-dependent Arf1 activation.
    Sheen V
    The Journal of neuroscience : the official journal of the Society for Neuroscience 33.40 (2013 Oct 2): 15735-46.

    Identification of ZO-1: a high molecular weight polypeptide associated with the tight junction (zonula occludens) in a variety of epithelia.
    Goodenough DA
    The Journal of cell biology 103.3 (1986 Sep): 755-66.

    CRTAM: A molecule involved in epithelial cell adhesion.
    González-Mariscal L
    Journal of cellular biochemistry 111.1 (2010 Sep 1): 111-22.

    ZO-1 recruitment to α-catenin--a novel mechanism for coupling the assembly of tight junctions to adherens junctions.
    DeMali KA
    Journal of cell science 126.Pt 17 (2013 Sep 1): 3904-15.

    A meckelin-filamin A interaction mediates ciliogenesis.
    Johnson CA
    Human molecular genetics 21.6 (2012 Mar 15): 1272-86.

    The stardust family protein MPP7 forms a tripartite complex with LIN7 and DLG1 that regulates the stability and localization of DLG1 to cell junctions.
    Vande Pol SB
    The Journal of biological chemistry 282.13 (2007 Mar 30): 9392-400.

    The SWI/SNF chromatin-remodeling complex subunit SNF5 is essential for hepatocyte differentiation.
    Klochendler-Yeivin A
    The EMBO journal 24.18 (2005 Sep 21): 3313-24.

    Expression of the unconventional myosin Myo1c alters sodium transport in M1 collecting duct cells.
    Bennett S
    American journal of physiology. Cell physiology 289.1 (2005 Jul): C120-9.

    Growth of choroid plexus epithelium vesicles in vitro depends on secretory activity.
    Ferretti P
    Journal of cellular physiology 208.3 (2006 Sep): 549-55.

    Ischemic acute renal failure induces the expression of a wide range of nephrogenic proteins.
    Vio CP
    American journal of physiology. Regulatory, integrative and comparative physiology 290.4 (2006 Apr): R861-70.

    Changes in the distribution of ZO-1, occludin, and claudins in the rat uterine epithelium during the estrous cycle.
    Cerbón M
    Cell and tissue research 319.2 (2005 Feb): 315-30.

    A unique cell population in the mouse olfactory bulb displays nuclear beta-catenin signaling during development and olfactory sensory neuron regeneration.
    Zhao H
    Developmental neurobiology 68.7 (2008 Jun): 859-69.

    ZO-2 silencing in epithelial cells perturbs the gate and fence function of tight junctions and leads to an atypical monolayer architecture.
    Gonzalez-Mariscal L
    Experimental cell research 313.8 (2007 May 1): 1533-47.

    Extrusion transplantation of Schwann cells into the adult rat thalamus induces directional host axon growth.
    Raisman G
    Experimental neurology 126.1 (1994 Mar): 31-43.

    C1 Esterase Inhibitor Reduces BBB Leakage and Apoptosis in the Hypoxic Developing Mouse Brain.
    Trollmann R
    Neuromolecular medicine 22.1 (2020 Mar): 31-44.

    IP References
    FFPE References

    Ischemic acute renal failure induces the expression of a wide range of nephrogenic proteins.
    Vio CP
    American journal of physiology. Regulatory, integrative and comparative physiology 290.4 (2006 Apr): R861-70.

    Angiogenic acceleration of Neu induced mammary tumor progression and metastasis.
    Cardiff RD
    Cancer research 64.1 (2004 Jan 1): 169-79.

    A meckelin-filamin A interaction mediates ciliogenesis.
    Johnson CA
    Human molecular genetics 21.6 (2012 Mar 15): 1272-86.

    All References

    Enteroendocrine cells are specifically marked by cell surface expression of claudin-4 in mouse small intestine.
    Hamazaki Y
    PloS one 9.6 (2014): e90638.

    Filamin A regulates neuronal migration through brefeldin A-inhibited guanine exchange factor 2-dependent Arf1 activation.
    Sheen V
    The Journal of neuroscience : the official journal of the Society for Neuroscience 33.40 (2013 Oct 2): 15735-46.

    Identification of ZO-1: a high molecular weight polypeptide associated with the tight junction (zonula occludens) in a variety of epithelia.
    Goodenough DA
    The Journal of cell biology 103.3 (1986 Sep): 755-66.

    CRTAM: A molecule involved in epithelial cell adhesion.
    González-Mariscal L
    Journal of cellular biochemistry 111.1 (2010 Sep 1): 111-22.

    ZO-1 recruitment to α-catenin--a novel mechanism for coupling the assembly of tight junctions to adherens junctions.
    DeMali KA
    Journal of cell science 126.Pt 17 (2013 Sep 1): 3904-15.

    A meckelin-filamin A interaction mediates ciliogenesis.
    Johnson CA
    Human molecular genetics 21.6 (2012 Mar 15): 1272-86.

    The stardust family protein MPP7 forms a tripartite complex with LIN7 and DLG1 that regulates the stability and localization of DLG1 to cell junctions.
    Vande Pol SB
    The Journal of biological chemistry 282.13 (2007 Mar 30): 9392-400.

    The SWI/SNF chromatin-remodeling complex subunit SNF5 is essential for hepatocyte differentiation.
    Klochendler-Yeivin A
    The EMBO journal 24.18 (2005 Sep 21): 3313-24.

    Expression of the unconventional myosin Myo1c alters sodium transport in M1 collecting duct cells.
    Bennett S
    American journal of physiology. Cell physiology 289.1 (2005 Jul): C120-9.

    Growth of choroid plexus epithelium vesicles in vitro depends on secretory activity.
    Ferretti P
    Journal of cellular physiology 208.3 (2006 Sep): 549-55.

    Ischemic acute renal failure induces the expression of a wide range of nephrogenic proteins.
    Vio CP
    American journal of physiology. Regulatory, integrative and comparative physiology 290.4 (2006 Apr): R861-70.

    Changes in the distribution of ZO-1, occludin, and claudins in the rat uterine epithelium during the estrous cycle.
    Cerbón M
    Cell and tissue research 319.2 (2005 Feb): 315-30.

    A unique cell population in the mouse olfactory bulb displays nuclear beta-catenin signaling during development and olfactory sensory neuron regeneration.
    Zhao H
    Developmental neurobiology 68.7 (2008 Jun): 859-69.

    ZO-2 silencing in epithelial cells perturbs the gate and fence function of tight junctions and leads to an atypical monolayer architecture.
    Gonzalez-Mariscal L
    Experimental cell research 313.8 (2007 May 1): 1533-47.

    Extrusion transplantation of Schwann cells into the adult rat thalamus induces directional host axon growth.
    Raisman G
    Experimental neurology 126.1 (1994 Mar): 31-43.

    C1 Esterase Inhibitor Reduces BBB Leakage and Apoptosis in the Hypoxic Developing Mouse Brain.
    Trollmann R
    Neuromolecular medicine 22.1 (2020 Mar): 31-44.

    Ectopic activation of Wnt/β-catenin signaling in lens fiber cells results in cataract formation and aberrant fiber cell differentiation.
    Kozmik Z
    PloS one 8.10 (2013): e78279.

    Hic-5 contributes to epithelial-mesenchymal transformation through a RhoA/ROCK-dependent pathway.
    Turner CE
    Journal of cellular physiology 211.3 (2007 Jun): 736-47.

    Micropattern-based platform as a physiologically relevant model to study epithelial morphogenesis and nephrotoxicity.
    Martin-Belmonte F
    Biomaterials 218. (2019 Oct): 119339.

    Smoothelin-like 2 Inhibits Coronin-1B to Stabilize the Apical Actin Cortex during Epithelial Morphogenesis.
    Martin-Belmonte F
    Current biology : CB 31.4 (2021 Feb 22): 696-706.e9.

    Characterization of ZO-1, a protein component of the tight junction from mouse liver and Madin-Darby canine kidney cells.
    Mooseker MS
    The Journal of cell biology 106.4 (1988 Apr): 1141-9.

    Angiogenic acceleration of Neu induced mammary tumor progression and metastasis.
    Cardiff RD
    Cancer research 64.1 (2004 Jan 1): 169-79.

    Trophic factors counteract elevated FGF-2-induced inhibition of adult neurogenesis.
    Grundke-Iqbal I
    Neurobiology of aging 28.8 (2007 Aug): 1148-62.

    Endogenous Nkx2.2+/Olig2+ oligodendrocyte precursor cells fail to remyelinate the demyelinated adult rat spinal cord in the absence of astrocytes.
    Whittemore SR
    Experimental neurology 192.1 (2005 Mar): 11-24.

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