4A5
Catalog Fields
Antigen: major sperm protein (MSP)
Available to For-Profits: Yes
Antigen Species: nematode
Hybridoma Cells Available (Non-Profit): Yes
Isotype: MIgG1, kappa light chain
Depositor: Greenstein, D.
Host Species: mouse
Antigen Sequence:
Positive Tested Species Reactivity: C. elegans, Nematode
Depositors Institution: University of Minnesota
Antigen Molecular Weight: Predicted: 14 kDa; Apparent: 15 kDa
Depositors Notes:
Immunogen: peptide (CGG-REWFQGDGMVRRKNLPIEYNP, aa 106-126 of MSP C-terminal) coupled to KLH
Gene: msp
Alternate Antibody Name:
Alternate Gene Names:
Alternate Antigen Name:
Clonality: Monoclonal
Myeloma Strain:
Epitope Mapped: Yes
Uniprot ID: P53017
Epitope Location or Sequence: C terminal (aa 106-126)
Entrez Gene ID: 177305
Immunogen Sequence: Peptide
Antibody Registry ID: AB_760348
Recommended Applications: Immunofluorescence, Immunoprecipitation, Western Blot
Additional Information: In C. elegans, MSP is encoded by a large multigene family. The MSP epitope recognized by 4A5 withstands aldehydye fixation, osmium post0fix and resin thin sesctioning.
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
4A5 was deposited to the DSHB by Greenstein, D. (DSHB Hybridoma Product 4A5)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
11 References
Initial Publication
C. elegans sperm bud vesicles to deliver a meiotic maturation signal to distant oocytes.
Greenstein D
Development (Cambridge, England) 132.15 (2005 Aug): 3357-69.