4A5

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SKU: 4A5

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DSHB Data Sheet

Catalog Fields

Product Name/ID: 4A5
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Name: msp
Ab Isotype: MIgG1, kappa light chain
Gene Symbol:
Antibody Registry ID: AB_760348 
Uniprot ID: P53017 
RRID:  
Entrez Gene ID: 177305 
Clonality: Monoclonal
Immunogen: peptide (CGG-REWFQGDGMVRRKNLPIEYNP, aa 106-126 of MSP C-terminal) coupled to KLH
Clone:
Immunogen Sequence: Peptide
Myeloma Strain:
Epitope Mapped: Yes
Antigen Name: major sperm protein (MSP)
Epitope Location or Sequence: C terminal (aa 106-126)
Alternate Antigen Name:
Deposit Date: 5/4/2006
Antigen Molecular Weight: Predicted: 14 kDa; Apparent: 15 kDa
Depositor: Greenstein, D.
Antigen Sequence:
Depositor Institution: University of Minnesota
Antigen Species: nematode
Depositor Notes:
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: C. elegans, Nematode
Additional Information: In C. elegans, MSP is encoded by a large multigene family. The MSP epitope recognized by 4A5 withstands aldehydye fixation, osmium post0fix and resin thin sesctioning.
Predicted Species Reactivity:  
Human Protein Atlas:  
Additional Characterization:  
Recommended Applications: Immunofluorescence, Immunoprecipitation, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
4A5 was deposited to the DSHB by Greenstein, D. (DSHB Hybridoma Product 4A5)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

11 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • Epitope Map References
  • All References
  • Initial Publication

    C. elegans sperm bud vesicles to deliver a meiotic maturation signal to distant oocytes.
    Greenstein D
    Development (Cambridge, England) 132.15 (2005 Aug): 3357-69.

    IF References

    C. elegans sperm bud vesicles to deliver a meiotic maturation signal to distant oocytes.
    Greenstein D
    Development (Cambridge, England) 132.15 (2005 Aug): 3357-69.

    Conservation of MAP kinase activity and MSP genes in parthenogenetic nematodes.
    Schierenberg E
    BMC developmental biology 10. (2010 May 17): 51.

    Sperm development and motility are regulated by PP1 phosphatases in Caenorhabditis elegans.
    Chu DS
    Genetics 190.1 (2012 Jan): 143-57.

    Evidence for phosphorylation in the MSP cytoskeletal filaments of amoeboid spermatozoa.
    Cardullo RA
    International journal of biochemistry and molecular biology 2.3 (2011): 263-73.

    Caenorhabditis elegans p97 controls germline-specific sex determination by controlling the TRA-1 level in a CUL-2-dependent manner.
    Yamanaka K
    Journal of cell science 122.Pt 20 (2009 Oct 15): 3663-72.

    Argonautes promote male fertility and provide a paternal memory of germline gene expression in C. elegans.
    Mello CC
    Cell 155.7 (2013 Dec 19): 1532-44.

    Caenorhabditis elegans UBX cofactors for CDC-48/p97 control spermatogenesis.
    Ogura T
    Genes to cells : devoted to molecular & cellular mechanisms 15.12 (2010 Dec): 1201-15.

    SACY-1 DEAD-Box helicase links the somatic control of oocyte meiotic maturation to the sperm-to-oocyte switch and gamete maintenance in Caenorhabditis elegans.
    Greenstein D
    Genetics 192.3 (2012 Nov): 905-28.

    Depletion of gipc-1 and gipc-2 causes infertility in Caenorhabditis elegans by reducing sperm motility.
    Shim YH
    Biochemical and biophysical research communications 534. (2021 Jan 1): 219-225.

    Membrane contact site-dependent cholesterol transport regulates Na(+)/K(+)-ATPase polarization and spermiogenesis in Caenorhabditis elegans.
    Miao L
    Developmental cell 56.11 (2021 Jun 7): 1631-1645.e7.

    WB References

    C. elegans sperm bud vesicles to deliver a meiotic maturation signal to distant oocytes.
    Greenstein D
    Development (Cambridge, England) 132.15 (2005 Aug): 3357-69.

    Conservation of MAP kinase activity and MSP genes in parthenogenetic nematodes.
    Schierenberg E
    BMC developmental biology 10. (2010 May 17): 51.

    Sperm development and motility are regulated by PP1 phosphatases in Caenorhabditis elegans.
    Chu DS
    Genetics 190.1 (2012 Jan): 143-57.

    Evidence for phosphorylation in the MSP cytoskeletal filaments of amoeboid spermatozoa.
    Cardullo RA
    International journal of biochemistry and molecular biology 2.3 (2011): 263-73.

    Depletion of gipc-1 and gipc-2 causes infertility in Caenorhabditis elegans by reducing sperm motility.
    Shim YH
    Biochemical and biophysical research communications 534. (2021 Jan 1): 219-225.

    IHC References
    Epitope Map References

    C. elegans sperm bud vesicles to deliver a meiotic maturation signal to distant oocytes.
    Greenstein D
    Development (Cambridge, England) 132.15 (2005 Aug): 3357-69.

    All References

    Ultrastructural immunogold localization of major sperm protein (MSP) in spermatogenic cells of the nematode Acrobeles complexus (Nematoda, Rhabditida).
    Bert W
    Micron (Oxford, England : 1993) 89. (2016 Oct): 43-55.

    C. elegans sperm bud vesicles to deliver a meiotic maturation signal to distant oocytes.
    Greenstein D
    Development (Cambridge, England) 132.15 (2005 Aug): 3357-69.

    Conservation of MAP kinase activity and MSP genes in parthenogenetic nematodes.
    Schierenberg E
    BMC developmental biology 10. (2010 May 17): 51.

    Sperm development and motility are regulated by PP1 phosphatases in Caenorhabditis elegans.
    Chu DS
    Genetics 190.1 (2012 Jan): 143-57.

    Evidence for phosphorylation in the MSP cytoskeletal filaments of amoeboid spermatozoa.
    Cardullo RA
    International journal of biochemistry and molecular biology 2.3 (2011): 263-73.

    Caenorhabditis elegans p97 controls germline-specific sex determination by controlling the TRA-1 level in a CUL-2-dependent manner.
    Yamanaka K
    Journal of cell science 122.Pt 20 (2009 Oct 15): 3663-72.

    Argonautes promote male fertility and provide a paternal memory of germline gene expression in C. elegans.
    Mello CC
    Cell 155.7 (2013 Dec 19): 1532-44.

    Caenorhabditis elegans UBX cofactors for CDC-48/p97 control spermatogenesis.
    Ogura T
    Genes to cells : devoted to molecular & cellular mechanisms 15.12 (2010 Dec): 1201-15.

    SACY-1 DEAD-Box helicase links the somatic control of oocyte meiotic maturation to the sperm-to-oocyte switch and gamete maintenance in Caenorhabditis elegans.
    Greenstein D
    Genetics 192.3 (2012 Nov): 905-28.

    Depletion of gipc-1 and gipc-2 causes infertility in Caenorhabditis elegans by reducing sperm motility.
    Shim YH
    Biochemical and biophysical research communications 534. (2021 Jan 1): 219-225.

    Membrane contact site-dependent cholesterol transport regulates Na(+)/K(+)-ATPase polarization and spermiogenesis in Caenorhabditis elegans.
    Miao L
    Developmental cell 56.11 (2021 Jun 7): 1631-1645.e7.

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