4A5

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$40.00
SKU: 4A5
View product citations for antibody 4A5 on CiteAb

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Available: 30

DSHB Data Sheet

Catalog Fields

Antigen: major sperm protein (MSP)
Hybridoma Cells Available: Yes
Antigen Species: nematode
Depositor: Greenstein, D.
Isotype: MIgG1, kappa light chain
Antigen Sequence:
Host Species: mouse
Depositors Institution: University of Minnesota
Positive Tested Species Reactivity: C. elegans, Nematode
Depositors Notes:
Antigen Molecular Weight: Predicted: 14 kDa; Apparent: 15 kDa
Human Protein Atlas:
Predicted Species Reactivity:  
Gene: msp
Immunogen: peptide (CGG-REWFQGDGMVRRKNLPIEYNP, aa 106-126 of MSP C-terminal) coupled to KLH
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain:
Epitope Location or Sequence: C terminal (aa 106-126)
Uniprot ID: P53017 
Immunogen Sequence: Peptide
Entrez Gene ID: 177305 
Additional Characterization:
Antibody Registry ID: AB_760348 
Additional Information: In C. elegans, MSP is encoded by a large multigene family. The MSP epitope recognized by 4A5 withstands aldehydye fixation, osmium post0fix and resin thin sesctioning.
Recommended Applications: Immunofluorescence, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
4A5 was deposited to the DSHB by Greenstein, D. (DSHB Hybridoma Product 4A5)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

9 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • Epitope Map References
  • All References
  • Initial Publication

    C. elegans sperm bud vesicles to deliver a meiotic maturation signal to distant oocytes.
    Greenstein D
    Development (Cambridge, England) 132.15 (2005 Aug): 3357-69.

    IF References
    WB References

    C. elegans sperm bud vesicles to deliver a meiotic maturation signal to distant oocytes.
    Greenstein D
    Development (Cambridge, England) 132.15 (2005 Aug): 3357-69.

    Conservation of MAP kinase activity and MSP genes in parthenogenetic nematodes.
    Schierenberg E
    BMC developmental biology 10. (2010 May 17): 51.

    Sperm development and motility are regulated by PP1 phosphatases in Caenorhabditis elegans.
    Chu DS
    Genetics 190.1 (2012 Jan): 143-57.

    Evidence for phosphorylation in the MSP cytoskeletal filaments of amoeboid spermatozoa.
    Cardullo RA
    International journal of biochemistry and molecular biology 2.3 (2011): 263-73.

    IHC References
    Epitope Map References

    C. elegans sperm bud vesicles to deliver a meiotic maturation signal to distant oocytes.
    Greenstein D
    Development (Cambridge, England) 132.15 (2005 Aug): 3357-69.

    All References

    Ultrastructural immunogold localization of major sperm protein (MSP) in spermatogenic cells of the nematode Acrobeles complexus (Nematoda, Rhabditida).
    Bert W
    Micron (Oxford, England : 1993) 89. (2016 Oct): 43-55.

    C. elegans sperm bud vesicles to deliver a meiotic maturation signal to distant oocytes.
    Greenstein D
    Development (Cambridge, England) 132.15 (2005 Aug): 3357-69.

    Conservation of MAP kinase activity and MSP genes in parthenogenetic nematodes.
    Schierenberg E
    BMC developmental biology 10. (2010 May 17): 51.

    Sperm development and motility are regulated by PP1 phosphatases in Caenorhabditis elegans.
    Chu DS
    Genetics 190.1 (2012 Jan): 143-57.

    Evidence for phosphorylation in the MSP cytoskeletal filaments of amoeboid spermatozoa.
    Cardullo RA
    International journal of biochemistry and molecular biology 2.3 (2011): 263-73.

    Caenorhabditis elegans p97 controls germline-specific sex determination by controlling the TRA-1 level in a CUL-2-dependent manner.
    Yamanaka K
    Journal of cell science 122.Pt 20 (2009 Oct 15): 3663-72.

    Argonautes promote male fertility and provide a paternal memory of germline gene expression in C. elegans.
    Mello CC
    Cell 155.7 (2013 Dec 19): 1532-44.

    Caenorhabditis elegans UBX cofactors for CDC-48/p97 control spermatogenesis.
    Ogura T
    Genes to cells : devoted to molecular and cellular mechanisms 15.12 (2010 Dec): 1201-15.

    SACY-1 DEAD-Box helicase links the somatic control of oocyte meiotic maturation to the sperm-to-oocyte switch and gamete maintenance in Caenorhabditis elegans.
    Greenstein D
    Genetics 192.3 (2012 Nov): 905-28.

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