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SKU: K76
View product citations for antibody K76 on CiteAb

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Available: 128

DSHB Data Sheet

Catalog Fields

Antigen: P granule abnormality protein 1
Hybridoma Cells Available: Yes
Antigen Species: C. elegans
Depositor: Strome, S. / Wood, W.B.
Isotype: MIgM
Antigen Sequence:
Host Species: mouse
Depositors Institution: University of California Santa Cruz/ University of Colorado
Positive Tested Species Reactivity: C. elegans
Depositors Notes: In adult hermaphrodites, K76 stains both germline granules and spermatheca.
Antigen Molecular Weight: 40 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: pgl-1
Immunogen: C. elegans embryo homogenate
Alternate Gene Names: ZK381.4
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: SP2/0
Epitope Location or Sequence:
Uniprot ID: Q9TZQ3 
Immunogen Sequence: Full length protein
Entrez Gene ID: 177461 
Additional Characterization:  
Antibody Registry ID: AB_531836 
Additional Information: RRID:AB_531836
Recommended Applications: Immunofluorescence, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
K76 was deposited to the DSHB by Strome, S. / Wood, W.B. (DSHB Hybridoma Product K76)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

7 References

  • Initial Publication
  • IF References
  • WB References
  • IP References
  • All References
  • Initial Publication
    IF References
    WB References
    IP References
    All References

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