(0) No Reviews yet
SKU: K76
View product citations for antibody K76 on CiteAb

In Stock

Available: 138

DSHB Data Sheet

Catalog Fields

Antigen: P granule abnormality protein 1
Hybridoma Cells Available: Yes
Antigen Species: C. elegans
Depositor: Strome, S. / Wood, W.B.
Isotype: MIgM
Antigen Sequence:
Host Species: mouse
Depositors Institution: University of California Santa Cruz/ University of Colorado
Positive Tested Species Reactivity: C. elegans
Depositors Notes: In adult hermaphrodites, K76 stains both germline granules and spermatheca.
Antigen Molecular Weight: 40 kDa
Human Protein Atlas:
Predicted Species Reactivity:  
Gene: pgl-1
Immunogen: C. elegans embryo homogenate
Alternate Gene Names: ZK381.4
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped:
Myeloma Strain: SP2/0
Epitope Location or Sequence:
Uniprot ID: Q9TZQ3 
Immunogen Sequence: Full length protein
Entrez Gene ID: 177461 
Additional Characterization:
Antibody Registry ID: AB_531836 
Additional Information: RRID:AB_531836
Recommended Applications: Immunofluorescence, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
K76 was deposited to the DSHB by Strome, S. / Wood, W.B. (DSHB Hybridoma Product K76)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

7 References

  • Initial Publication
  • IF References
  • WB References
  • IP References
  • All References
  • Initial Publication
    IF References
    WB References
    IP References
    All References

    Ratings & Reviews

    No reviews available

    Be the first to Write a Review