4E2(3G2)

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$40.00
SKU: 4E2(3G2)-s
View product citations for antibody 4E2(3G2) on CiteAb

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Available: 27

DSHB Data Sheet

Catalog Fields

Antigen: neuromuscular junction and reactive Schwann cell associated antigen
Hybridoma Cells Available: No
Antigen Species: rat
Depositor: Thompson, W.J.
Isotype: MIgG2b
Antigen Sequence:
Host Species: mouse
Depositors Institution: University of Texas, Austin
Positive Tested Species Reactivity: Rat
Depositors Notes: Immunohistology: cytoskeleton of L6 and L8 cell lines; neuromuscular and myotendonous junctions; reactive Schwann cells. Antigen chemical properties: presumed cytoskeletal protein. Purification note: enriched in cytoskeletal preparations.
Antigen Molecular Weight: 46 kDa (46 kDa and 48 kDa in cell lines)
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene:
Immunogen: membrane vesicles from lower hind-limb muscles
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: NS-1
Epitope Location or Sequence:
Uniprot ID:  
Immunogen Sequence:
Entrez Gene ID:  
Additional Characterization:  
Antibody Registry ID:  
Additional Information:
Recommended Applications: Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
4E2(3G2) was deposited to the DSHB by Thompson, W.J. (DSHB Hybridoma Product 4E2(3G2))
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

1 Reference

  • Initial Publication
  • All References
  • Initial Publication

    Developmental and neural regulation of a subsarcolemmal component of the rat neuromuscular junction.
    Thompson WJ
    The Journal of neuroscience : the official journal of the Society for Neuroscience 12.5 (1992 May): 1602-15.

    All References

    Developmental and neural regulation of a subsarcolemmal component of the rat neuromuscular junction.
    Thompson WJ
    The Journal of neuroscience : the official journal of the Society for Neuroscience 12.5 (1992 May): 1602-15.

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