4E2(3G2)

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$45.00
SKU: 4E2(3G2)-s
View product citations for antibody 4E2(3G2) on CiteAb

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Available: 36

DSHB Data Sheet

Catalog Fields

Antigen: neuromuscular junction and reactive Schwann cell associated antigen
Available to For-Profits: Yes
Antigen Species: rat
Hybridoma Cells Available (Non-Profit): No
Isotype: MIgG2b
Depositor: Thompson, W.J.
Host Species: mouse
Antigen Sequence:
Positive Tested Species Reactivity: Rat
Depositors Institution: University of Texas, Austin
Antigen Molecular Weight: 46 kDa (46 kDa and 48 kDa in cell lines)
Depositors Notes: Immunohistology: cytoskeleton of L6 and L8 cell lines; neuromuscular and myotendonous junctions; reactive Schwann cells. Antigen chemical properties: presumed cytoskeletal protein. Purification note: enriched in cytoskeletal preparations.
Predicted Species Reactivity:  
Human Protein Atlas:  
Immunogen: membrane vesicles from lower hind-limb muscles
Gene:
Alternate Antibody Name:
Alternate Gene Names:
Alternate Antigen Name:
Clonality: Monoclonal
Myeloma Strain: NS-1
Epitope Mapped: No
Uniprot ID:  
Epitope Location or Sequence:
Entrez Gene ID:  
Immunogen Sequence:
Antibody Registry ID: AB_2276109 
Additional Characterization:  
Recommended Applications: Western Blot
Additional Information:
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
4E2(3G2) was deposited to the DSHB by Thompson, W.J. (DSHB Hybridoma Product 4E2(3G2))
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

1 Reference

  • Initial Publication
  • All References
  • Initial Publication

    Developmental and neural regulation of a subsarcolemmal component of the rat neuromuscular junction.
    Thompson WJ
    The Journal of neuroscience : the official journal of the Society for Neuroscience 12.5 (1992 May): 1602-15.

    All References

    Developmental and neural regulation of a subsarcolemmal component of the rat neuromuscular junction.
    Thompson WJ
    The Journal of neuroscience : the official journal of the Society for Neuroscience 12.5 (1992 May): 1602-15.

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