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SKU: 56.4H7

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DSHB Data Sheet

Catalog Fields

Product Name/ID: 56.4H7
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Name:
Ab Isotype: MIgG2a
Gene Symbol:
Antibody Registry ID: AB_2179111 
Uniprot ID:  
Entrez Gene ID:  
Clonality: Monoclonal
Immunogen: rhoB; DHFR-6-his fusion in E. coli
Immunogen Sequence:
Myeloma Strain: NS-1
Epitope Mapped: No
Antigen Name: rhoB
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 2/12/1999
Antigen Molecular Weight:
Depositor: Jessell, T.M. / Brenner-Morton, S.
Antigen Sequence:
Depositor Institution: Columbia University
Antigen Species: chick
Depositor Notes: Immunohistology note: neural crest (dorsal neural tube and early migrating cells). IHC note: sections are treated with 6M guanidine HCL in 0.1 M PB for 10 minutes @ 20°C wash with PBS and block with 10% goat serum and 0.1% Tx 100 in PBS.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Chick
Additional Information:
Predicted Species Reactivity:  
Human Protein Atlas:  
Additional Characterization:  
Recommended Applications:  
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
56.4H7 was deposited to the DSHB by Jessell, T.M. / Brenner-Morton, S. (DSHB Hybridoma Product 56.4H7)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

1 Reference

  • Initial Publication
  • All References
  • Initial Publication

    A role for rhoB in the delamination of neural crest cells from the dorsal neural tube.
    Jessell TM
    Development (Cambridge, England) 125.24 (1998 Dec): 5055-67.

    All References

    A role for rhoB in the delamination of neural crest cells from the dorsal neural tube.
    Jessell TM
    Development (Cambridge, England) 125.24 (1998 Dec): 5055-67.

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