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SKU: 62.1E6

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DSHB Data Sheet

Catalog Fields

Product Name/ID: 62.1E6
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Name: Snai2
Ab Isotype: MIgG1
Gene Symbol:
Antibody Registry ID: AB_528471 
Uniprot ID: Q91051 
Entrez Gene ID: 432368 
Clonality: Monoclonal
Immunogen: Slug (chick)
Immunogen Sequence: 6-his full length protein
Myeloma Strain: NS-1
Epitope Mapped: No
Antigen Name: Slug
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 10/30/1997
Antigen Molecular Weight: 30 kDa
Depositor: Jessell, T.M. / Brenner-Morton, S.
Antigen Sequence:
Depositor Institution: Columbia University
Antigen Species: chicken
Depositor Notes: Antigen chemical properties: protein/transcription factor. Cell binding note: nuclear. Immunohistology note: neural crest.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Chicken, Human, Xenopus
Additional Information:
Predicted Species Reactivity:  
Human Protein Atlas:  
Additional Characterization:  
Recommended Applications: Immunohistochemistry
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
62.1E6 was deposited to the DSHB by Jessell, T.M. / Brenner-Morton, S. (DSHB Hybridoma Product 62.1E6)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

2 References

  • Initial Publication
  • IHC References
  • All References
  • Initial Publication
    IHC References

    Elk3 is essential for the progression from progenitor to definitive neural crest cell.
    Bronner ME
    Developmental biology 374.2 (2013 Feb 15): 255-63.

    All References

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