62.1E6

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$40.00
SKU: 62.1E6
View product citations for antibody 62.1E6 on CiteAb

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Available: 99

DSHB Data Sheet

Catalog Fields

Antigen: Slug
Hybridoma Cells Available: Yes
Antigen Species: chicken
Depositor: Jessell, T.M. / Brenner-Morton, S.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: Columbia University
Positive Tested Species Reactivity: Chicken, Human, Xenopus
Depositors Notes: Antigen chemical properties: protein/transcription factor. Cell binding note: nuclear. Immunohistology note: neural crest.
Antigen Molecular Weight: 30 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene:
Immunogen: Slug (chick)
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: NS-1
Epitope Location or Sequence:
Uniprot ID:  
Immunogen Sequence: 6-his full length protein
Entrez Gene ID:  
Additional Characterization:  
Antibody Registry ID:  
Additional Information:
Recommended Applications: Immunohistochemistry
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
62.1E6 was deposited to the DSHB by Jessell, T.M. / Brenner-Morton, S. (DSHB Hybridoma Product 62.1E6)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

2 References

  • Initial Publication
  • IHC References
  • All References
  • Initial Publication
    IHC References

    Elk3 is essential for the progression from progenitor to definitive neural crest cell.
    Bronner ME
    Developmental biology 374.2 (2013 Feb 15): 255-63.

    All References

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