6B7

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SKU: 6B7

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DSHB Data Sheet

Catalog Fields

Clone ID/Product Name: 6B7
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Symbol:
Ab Isotype: MIgG2b
Gene Name:
Antibody Registry ID: AB_2827392 
Uniprot ID:  
RRID:  
Entrez Gene ID:  
Clonality: Monoclonal
Immunogen:
Clone:
Immunogen Sequence:
Myeloma Strain: Fox-NY cells
Epitope Mapped: No
Antigen Name: ecdysone receptor (EcR) B1-isoform specific, Manduca sexta
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 2/11/1998
Antigen Molecular Weight: 64 kDa (predicted); between 70-80 kDa by immunoblotting
Depositor: Riddiford, L.M.
Antigen Sequence:
Depositor Institution: University of Washington
Antigen Species: Manduca sexta
Depositor Notes: Species specificity note: Manduca; Bombyx mori; Locusta migratoria (?). Specific to the region between amino acids 33-110 of EcR-B1 (Fujiwara et al., 1995) (B1-specific) as determined by immunoblotting against various EcR fusion proteins (Jindra et al., 1996.). Functional effects note: nuclear receptor for ecdysone, the molting hormone of insects.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Bombyx mori, Manduca
Additional Information:
Predicted Species Reactivity:  
Human Protein Atlas:  
Additional Characterization:  
Recommended Applications: Immunohistochemistry, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
6B7 was deposited to the DSHB by Riddiford, L.M. (DSHB Hybridoma Product 6B7)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

2 References

  • Initial Publication
  • WB References
  • IHC References
  • All References
  • Initial Publication

    Cloning of an ecdysone receptor homolog from Manduca sexta and the developmental profile of its mRNA in wings.
    Riddiford LM
    Insect biochemistry and molecular biology 25.7 (1995 Jul): 845-56.

    WB References

    Cloning of an ecdysone receptor homolog from Manduca sexta and the developmental profile of its mRNA in wings.
    Riddiford LM
    Insect biochemistry and molecular biology 25.7 (1995 Jul): 845-56.

    IHC References
    All References

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