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SKU: 10F1
View product citations for antibody 10F1 on CiteAb

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Available: 36

DSHB Data Sheet

Catalog Fields

Antigen: ecdysone receptor (EcR) (common), Manduca sexta
Hybridoma Cells Available: Yes
Antigen Species: Manduca sexta
Depositor: Riddiford, L.M.
Isotype: MIgG2b, kappa light chain
Antigen Sequence: amino acids 33-454 of EcR-B1
Host Species: mouse
Depositors Institution: Janelia Farm Research Campus
Positive Tested Species Reactivity: Bombyx mori, Drosophila, Locusta migratoria, Manduca, Rhodnius prolixus
Depositors Notes: 10F1 is used in the ecodysone-induced gene expression system
Antigen Molecular Weight: Predicted: 62.8kDa; Apparent: 70-80kDa
Human Protein Atlas:
Predicted Species Reactivity:  
Gene: EcR
Immunogen: E. coli: fusion protein of EcR-B1 (amino acids 33-454) fused to glutathione-S-transferase; only the EcR-B1 portion of the fusion protein injected
Alternate Gene Names: NR1H1
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: Fox-NY
Epitope Location or Sequence: amino acids 127-354 of EcR-B1
Uniprot ID: P49883 
Immunogen Sequence: amino acids 33-454 of EcR-B1
Entrez Gene ID: 35540 
Additional Characterization:
Antibody Registry ID: AB_528210 
Additional Information:
Recommended Applications: Immunofluorescence, Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
10F1 was deposited to the DSHB by Riddiford, L.M. (DSHB Hybridoma Product 10F1)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

4 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • Epitope Map References
  • All References
  • Initial Publication
    IF References
    WB References
    IHC References
    Epitope Map References
    All References

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