Antibody Name: 10F1
Antigen:ecdysone receptor (EcR) (common), Manduca sexta
IF of a Drosophila 5th instar larval hindwing stained with DSHB antibody 10F1 against EcR (green). Image credit: Antónia Monteiro, PMID: 26405828, Fig. 4C
Catalog Fields
Identity
Clone ID / Antibody Name: 10F1
Alternate Antibody Name: —
Antigen Name: ecdysone receptor (EcR) (common), Manduca sexta
Alternate Antigen Name: —
Gene Symbol: —
Gene Name: EcR
Entrez Gene ID: 35540
Antigen Sequence: amino acids 33-454 of EcR-B1
Antigen Species: Manduca sexta
Antigen Mol. Weight: Predicted: 62.8kDa; Apparent: 70-80kDa
Uniprot ID: P49883
Human Protein Atlas: —
Antibody Properties
Host Species: Mouse
Clonality: Monoclonal
Isotype: Mouse IgG2b, kappa light chain
Myeloma Strain: Fox-NY
Antibody Registry / RRID: AB_528210
Available to For-Profits: Yes
Hybridoma Available: Yes
Cell lines cannot be distributed to commercial/for-profit users.
Cell lines cannot be distributed to commercial/for-profit users.
Immunogen & Epitope
Immunogen: E. coli: fusion protein of EcR-B1 (amino acids 33-454) fused to glutathione-S-transferase; only the EcR-B1 portion of the fusion protein injected
Immunogen Sequence: amino acids 33-454 of EcR-B1
Epitope Mapped: Yes
Epitope Location / Seq: amino acids 127-354 of EcR-B1
Clone: —
Additional Info: —
Add. Characterization: —
Species Reactivity
Confirmed Reactivity: Bombyx mori, Drosophila, Locusta migratoria, Manduca, Rhodnius prolixus
Predicted Reactivity: —
Depositor
Depositor Name: L.M. Riddiford
Institution: Janelia Farm Research Campus
Deposit Date: February 11, 1998
Depositor Notes: 10F1 is used in the ecodysone-induced gene expression system
Recommended Applications
IF
IHC
WB
Each lab must optimize concentration per application and species. Full usage recommendations →
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