(0) No Reviews yet
$45.00 to $425.00
SKU: 10F1
View product citations for antibody 10F1 on CiteAb

In Stock

Available: 15

DSHB Data Sheet

Catalog Fields

Antigen: ecdysone receptor (EcR) (common), Manduca sexta
Hybridoma Cells Available: Yes
Antigen Species: Manduca sexta
Depositor: Riddiford, L.M.
Isotype: MIgG2b, kappa light chain
Antigen Sequence: amino acids 33-454 of EcR-B1
Host Species: mouse
Depositors Institution: Janelia Farm Research Campus
Positive Tested Species Reactivity: Bombyx mori, Drosophila, Locusta migratoria, Manduca, Rhodnius prolixus
Depositors Notes: 10F1 is used in the ecodysone-induced gene expression system
Antigen Molecular Weight: Predicted: 62.8kDa; Apparent: 70-80kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: EcR
Immunogen: E. coli: fusion protein of EcR-B1 (amino acids 33-454) fused to glutathione-S-transferase; only the EcR-B1 portion of the fusion protein injected
Alternate Gene Names: NR1H1
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: Fox-NY
Epitope Location or Sequence: amino acids 127-354 of EcR-B1
Uniprot ID: P49883 
Immunogen Sequence: amino acids 33-454 of EcR-B1
Entrez Gene ID: 35540 
Additional Characterization:  
Antibody Registry ID: AB_528210 
Additional Information:
Recommended Applications: Immunofluorescence, Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
10F1 was deposited to the DSHB by Riddiford, L.M. (DSHB Hybridoma Product 10F1)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
All cell products contain the antimicrobial ProClin. Click here for additional information.

4 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • Epitope Map References
  • All References
  • Initial Publication
    IF References
    WB References
    IHC References
    Epitope Map References
    All References

    Ratings & Reviews

    No reviews available

    Be the first to Write a Review