9B9

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$40.00
SKU: 9B9
View product citations for antibody 9B9 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: ecdysone receptor (EcR) (common), Manduca sexta
Hybridoma Cells Available: Yes
Antigen Species: Manduca sexta
Depositor: Riddiford, L.M.
Isotype: MIgG2b
Antigen Sequence:
Host Species: mouse
Depositors Institution: University of Washington
Positive Tested Species Reactivity: Bombyx mori, Cricket, Manduca
Depositors Notes: Specificity note: Specific to the region between amino acids 127-354 of EcR-B1 (Fujiwara et al., 1995) as determined by immunoblotting against various EcR fusion proteins. Functional effects: nuclear receptor for ecdysone, the molting hormone of insects.
Antigen Molecular Weight: 64 kDa (predicted); between 70-80 kDa by immunoblotting
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene:
Immunogen:
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: Fox-NY cells
Epitope Location or Sequence:
Uniprot ID:  
Immunogen Sequence:
Entrez Gene ID:  
Additional Characterization:  
Antibody Registry ID:  
Additional Information:
Recommended Applications: Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
9B9 was deposited to the DSHB by Riddiford, L.M. (DSHB Hybridoma Product 9B9)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

1 Reference

  • Initial Publication
  • WB References
  • All References
  • Initial Publication

    Cloning of an ecdysone receptor homolog from Manduca sexta and the developmental profile of its mRNA in wings.
    Riddiford LM
    Insect biochemistry and molecular biology 25.7 (1995 Jul): 845-56.

    WB References

    Cloning of an ecdysone receptor homolog from Manduca sexta and the developmental profile of its mRNA in wings.
    Riddiford LM
    Insect biochemistry and molecular biology 25.7 (1995 Jul): 845-56.

    All References

    Cloning of an ecdysone receptor homolog from Manduca sexta and the developmental profile of its mRNA in wings.
    Riddiford LM
    Insect biochemistry and molecular biology 25.7 (1995 Jul): 845-56.

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