B10

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$40.00
SKU: B10
View product citations for antibody B10 on CiteAb

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Available: 30

DSHB Data Sheet

Catalog Fields

Antigen: neurofilaments, primary sensory and motor neurons
Hybridoma Cells Available: Yes
Antigen Species: Mouse
Depositor: Gilbert, W. / Stainier, D.
Isotype: MIgM
Antigen Sequence:
Host Species: mouse
Depositors Institution: Max Planck Institute for Heart & Lung Research
Positive Tested Species Reactivity: Mouse, Rat
Depositors Notes: filaments in maturing primary sensory and motor neurons
Antigen Molecular Weight:
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene:
Immunogen: Whole murine cells from the mid- and hindbrain (development stage: E18)
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: FOX-NY
Epitope Location or Sequence:
Uniprot ID:  
Immunogen Sequence: Full length protein
Entrez Gene ID:  
Additional Characterization:  
Antibody Registry ID: AB_531875 
Additional Information: The B10 epitope is developmentally regulated. B10 recognizes a cytoplasmic epitope expressed only in the E1.9 immunoreactive subset of neurons after E1.9 immunoreactivity disappears (see mAb E1.9). It is not clear which filaments are recognized by this antibody. For this reason we didn't link it to a Uniprot or gene ID.
Recommended Applications: Immunofluorescence
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
B10 was deposited to the DSHB by Gilbert, W. / Stainier, D. (DSHB Hybridoma Product B10)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

1 Reference

  • Initial Publication
  • IF References
  • All References
  • Initial Publication

    Neuronal differentiation and maturation in the mouse trigeminal sensory system, in vivo and in vitro.
    Gilbert W
    The Journal of comparative neurology 311.2 (1991 Sep 8): 300-12.

    IF References

    Neuronal differentiation and maturation in the mouse trigeminal sensory system, in vivo and in vitro.
    Gilbert W
    The Journal of comparative neurology 311.2 (1991 Sep 8): 300-12.

    All References

    Neuronal differentiation and maturation in the mouse trigeminal sensory system, in vivo and in vitro.
    Gilbert W
    The Journal of comparative neurology 311.2 (1991 Sep 8): 300-12.

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