B2C11
Catalog Fields
Antigen: CD9
Hybridoma Cells Available: Yes
Antigen Species: Rat
Depositor: Patterson, P.H.
Isotype: MIgG2c
Antigen Sequence:
Host Species: mouse
Depositors Institution: Caltech
Positive Tested Species Reactivity: Human, Mouse, Rat
Depositors Notes: The B2C11 monoclonal antibody activates Schwann cells and neurons (PMID: 7529830).
Predicted Species Reactivity:
Gene: Cd9
Immunogen: NG108 cells (mouse neuroblastoma N18 X rat glioma C6 hybrid)
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: P3X63Ag8.653
Epitope Location or Sequence: Extracellular domain of CD9
Uniprot ID: P40241
Immunogen Sequence: Full length sequence
Entrez Gene ID: 24936
Antibody Registry ID: AB_528160
Additional Information: RRID: AB_528160
Recommended Applications: FACS, Function Blocking, Immunofluorescence, Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
B2C11 was deposited to the DSHB by Patterson, P.H. (DSHB Hybridoma Product B2C11)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.
12 References
Initial Publication
Detection of a cell surface antigen found on rat peripheral nervous system neurons and multiple glia: astrocytes, oligodendrocytes, and Schwann cells.
Warren SL
Journal of neuroscience research 12.1 (1984): 41-57.