B2C11
Catalog Fields
Product Name/ID: B2C11
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Name: Cd9
Ab Isotype: MIgG2c
Gene Symbol:
Entrez Gene ID: 24936
Clonality: Monoclonal
Immunogen: NG108 cells (mouse neuroblastoma N18 X rat glioma C6 hybrid)
Clone:
Immunogen Sequence: Full length sequence
Myeloma Strain: P3X63Ag8.653
Epitope Mapped: Yes
Antigen Name: CD9
Epitope Location or Sequence: Extracellular domain of CD9
Alternate Antigen Name:
Deposit Date: 10/22/1998
Antigen Molecular Weight: 25.2 kDa
Depositor: Patterson, P.H.
Antigen Sequence:
Depositor Institution: California Institute of Technology
Antigen Species: Rat
Depositor Notes: The B2C11 monoclonal antibody activates Schwann cells and neurons (PMID: 7529830).
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Human, Mouse, Rat
Additional Information: RRID: AB_528160
Recommended Applications: FACS, Function Blocking, Immunofluorescence, Immunohistochemistry, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
B2C11 was deposited to the DSHB by Patterson, P.H. (DSHB Hybridoma Product B2C11)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
12 References
Initial Publication
Detection of a cell surface antigen found on rat peripheral nervous system neurons and multiple glia: astrocytes, oligodendrocytes, and Schwann cells.
Warren SL
Journal of neuroscience research 12.1 (1984): 41-57.