B2C11

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$40.00
SKU: B2C11
View product citations for antibody B2C11 on CiteAb

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Available: 126

DSHB Data Sheet

Catalog Fields

Antigen: CD9
Hybridoma Cells Available: Yes
Antigen Species: Rat
Depositor: Patterson, P.H.
Isotype: MIgG2c
Antigen Sequence:
Host Species: mouse
Depositors Institution: Caltech
Positive Tested Species Reactivity: Human, Mouse, Rat
Depositors Notes: The B2C11 monoclonal antibody activates Schwann cells and neurons (PMID: 7529830).
Antigen Molecular Weight: 25.2 kDa
Human Protein Atlas:
Predicted Species Reactivity:  
Gene: Cd9
Immunogen: NG108 cells (mouse neuroblastoma N18 X rat glioma C6 hybrid)
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: P3X63Ag8.653
Epitope Location or Sequence: Extracellular domain of CD9
Uniprot ID: P40241 
Immunogen Sequence: Full length sequence
Entrez Gene ID: 24936 
Additional Characterization:
Antibody Registry ID: AB_528160 
Additional Information: RRID: AB_528160
Recommended Applications: FACS, Function Blocking, Immunofluorescence, Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
B2C11 was deposited to the DSHB by Patterson, P.H. (DSHB Hybridoma Product B2C11)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

12 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • IP References
  • FB References
  • FACS References
  • Epitope Map References
  • All References
  • Initial Publication
    IF References

    Association of the tetraspan protein CD9 with integrins on the surface of S-16 Schwann cells.
    Patterson PH
    Journal of neurochemistry 67.6 (1996 Dec): 2505-13.

    Detection of a cell surface antigen found on rat peripheral nervous system neurons and multiple glia: astrocytes, oligodendrocytes, and Schwann cells.
    Warren SL
    Journal of neuroscience research 12.1 (1984): 41-57.

    An anti-CD9 monoclonal antibody promotes adhesion and induces proliferation of Schwann cells in vitro.
    Patterson PH
    The Journal of neuroscience : the official journal of the Society for Neuroscience 15.1 Pt 2 (1995 Jan): 574-83.

    An antibody to the tetraspan membrane protein CD9 promotes neurite formation in a partially alpha3beta1 integrin-dependent manner.
    Patterson PH
    The Journal of neuroscience : the official journal of the Society for Neuroscience 17.8 (1997 Apr 15): 2756-65.

    Surface protein characterization of human adipose tissue-derived stromal cells.
    Gimble JM
    Journal of cellular physiology 189.1 (2001 Oct): 54-63.

    Coexpression of CD9 augments the ability of membrane-bound heparin-binding epidermal growth factor-like growth factor (proHB-EGF) to preserve renal epithelial cell viability.
    Harris RC
    Kidney international 55.1 (1999 Jan): 71-81.

    The association of the tetraspanin D6.1A with the alpha6beta4 integrin supports cell motility and liver metastasis formation.
    Zöller M
    Journal of cell science 116.Pt 21 (2003 Nov 1): 4373-90.

    Characterization of trophoblast and extraembryonic endoderm cell lineages derived from rat preimplantation embryos.
    Krivokharchenko A
    PloS one 5.3 (2010 Mar 29): e9794.

    WB References

    Detection of a cell surface antigen found on rat peripheral nervous system neurons and multiple glia: astrocytes, oligodendrocytes, and Schwann cells.
    Warren SL
    Journal of neuroscience research 12.1 (1984): 41-57.

    An anti-CD9 monoclonal antibody promotes adhesion and induces proliferation of Schwann cells in vitro.
    Patterson PH
    The Journal of neuroscience : the official journal of the Society for Neuroscience 15.1 Pt 2 (1995 Jan): 574-83.

    CD9, a major platelet cell surface glycoprotein, is a ROCA antigen and is expressed in the nervous system.
    Patterson PH
    The Journal of neuroscience : the official journal of the Society for Neuroscience 15.1 Pt 2 (1995 Jan): 562-73.

    Association of the tetraspan protein CD9 with integrins on the surface of S-16 Schwann cells.
    Patterson PH
    Journal of neurochemistry 67.6 (1996 Dec): 2505-13.

    IHC References

    Surface protein characterization of human adipose tissue-derived stromal cells.
    Gimble JM
    Journal of cellular physiology 189.1 (2001 Oct): 54-63.

    CD9, a major platelet cell surface glycoprotein, is a ROCA antigen and is expressed in the nervous system.
    Patterson PH
    The Journal of neuroscience : the official journal of the Society for Neuroscience 15.1 Pt 2 (1995 Jan): 562-73.

    IP References
    FB References

    An anti-CD9 monoclonal antibody promotes adhesion and induces proliferation of Schwann cells in vitro.
    Patterson PH
    The Journal of neuroscience : the official journal of the Society for Neuroscience 15.1 Pt 2 (1995 Jan): 574-83.

    An antibody to the tetraspan membrane protein CD9 promotes neurite formation in a partially alpha3beta1 integrin-dependent manner.
    Patterson PH
    The Journal of neuroscience : the official journal of the Society for Neuroscience 17.8 (1997 Apr 15): 2756-65.

    CD9 plays a role in Schwann cell migration in vitro.
    Matthew WD
    The Journal of neuroscience : the official journal of the Society for Neuroscience 15.1 Pt 2 (1995 Jan): 584-95.

    FACS References

    Surface protein characterization of human adipose tissue-derived stromal cells.
    Gimble JM
    Journal of cellular physiology 189.1 (2001 Oct): 54-63.

    CD44 variant isoforms associate with tetraspanins and EpCAM.
    Zöller M
    Experimental cell research 297.2 (2004 Jul 15): 329-47.

    Monoclonal antibodies against rat leukocyte surface antigens.
    Dijkstra CD
    Immunological reviews 184. (2001 Dec): 109-16.

    Epitope Map References

    CD9, a major platelet cell surface glycoprotein, is a ROCA antigen and is expressed in the nervous system.
    Patterson PH
    The Journal of neuroscience : the official journal of the Society for Neuroscience 15.1 Pt 2 (1995 Jan): 562-73.

    All References

    Surface protein characterization of human adipose tissue-derived stromal cells.
    Gimble JM
    Journal of cellular physiology 189.1 (2001 Oct): 54-63.

    CD9, a major platelet cell surface glycoprotein, is a ROCA antigen and is expressed in the nervous system.
    Patterson PH
    The Journal of neuroscience : the official journal of the Society for Neuroscience 15.1 Pt 2 (1995 Jan): 562-73.

    Association of the tetraspan protein CD9 with integrins on the surface of S-16 Schwann cells.
    Patterson PH
    Journal of neurochemistry 67.6 (1996 Dec): 2505-13.

    Detection of a cell surface antigen found on rat peripheral nervous system neurons and multiple glia: astrocytes, oligodendrocytes, and Schwann cells.
    Warren SL
    Journal of neuroscience research 12.1 (1984): 41-57.

    An anti-CD9 monoclonal antibody promotes adhesion and induces proliferation of Schwann cells in vitro.
    Patterson PH
    The Journal of neuroscience : the official journal of the Society for Neuroscience 15.1 Pt 2 (1995 Jan): 574-83.

    An antibody to the tetraspan membrane protein CD9 promotes neurite formation in a partially alpha3beta1 integrin-dependent manner.
    Patterson PH
    The Journal of neuroscience : the official journal of the Society for Neuroscience 17.8 (1997 Apr 15): 2756-65.

    Coexpression of CD9 augments the ability of membrane-bound heparin-binding epidermal growth factor-like growth factor (proHB-EGF) to preserve renal epithelial cell viability.
    Harris RC
    Kidney international 55.1 (1999 Jan): 71-81.

    The association of the tetraspanin D6.1A with the alpha6beta4 integrin supports cell motility and liver metastasis formation.
    Zöller M
    Journal of cell science 116.Pt 21 (2003 Nov 1): 4373-90.

    Characterization of trophoblast and extraembryonic endoderm cell lineages derived from rat preimplantation embryos.
    Krivokharchenko A
    PloS one 5.3 (2010 Mar 29): e9794.

    CD9 plays a role in Schwann cell migration in vitro.
    Matthew WD
    The Journal of neuroscience : the official journal of the Society for Neuroscience 15.1 Pt 2 (1995 Jan): 584-95.

    CD44 variant isoforms associate with tetraspanins and EpCAM.
    Zöller M
    Experimental cell research 297.2 (2004 Jul 15): 329-47.

    Monoclonal antibodies against rat leukocyte surface antigens.
    Dijkstra CD
    Immunological reviews 184. (2001 Dec): 109-16.

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