B2C11
Catalog Fields
Antigen: CD9
Hybridoma Cells Available: Yes
Antigen Species: Rat
Depositor: Patterson, P.H.
Isotype: MIgG2c
Antigen Sequence:
Host Species: mouse
Depositors Institution: California Institute of Technology
Positive Tested Species Reactivity: Human, Mouse, Rat
Depositors Notes: The B2C11 monoclonal antibody activates Schwann cells and neurons (PMID: 7529830).
Predicted Species Reactivity:
Gene: Cd9
Immunogen: NG108 cells (mouse neuroblastoma N18 X rat glioma C6 hybrid)
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: P3X63Ag8.653
Epitope Location or Sequence: Extracellular domain of CD9
Uniprot ID: P40241
Immunogen Sequence: Full length sequence
Entrez Gene ID: 24936
Antibody Registry ID: AB_528160
Additional Information: RRID: AB_528160
Recommended Applications: FACS, Function Blocking, Immunofluorescence, Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
B2C11 was deposited to the DSHB by Patterson, P.H. (DSHB Hybridoma Product B2C11)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
All cell products contain the antimicrobial ProClin. Click here for additional information.
12 References
Initial Publication
Detection of a cell surface antigen found on rat peripheral nervous system neurons and multiple glia: astrocytes, oligodendrocytes, and Schwann cells.
Warren SL
Journal of neuroscience research 12.1 (1984): 41-57.